Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation

Microtubule-associated protein 1B (MAP1B) is prominently expressed during early stages of neuronal development, and it has been implicated in axonal growth and guidance. MAP1B expression is also found in the adult brain in areas of significant synaptic plasticity. Here, we demonstrate that MAP1B is...

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Autores: Tortosa Binacua, Elena, Montenegro Venegas, Carolina, Benoist, Marion, Härtel, Steffen, González Billault, Christian, Rodríguez Esteban, José A., Avila, Jesús
Tipo de documento: artigo
Data de publicação:2011
País:España
Recursos:Universidad Autónoma de Madrid
Repositório:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglês
OAI Identifier:oai:repositorio.uam.es:10486/717717
Acesso em linha:http://hdl.handle.net/10486/717717
https://dx.doi.org/10.1074/jbc.M111.271320
Access Level:Acceso aberto
Palavra-chave:Farmacia
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spelling Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturationTortosa Binacua, ElenaMontenegro Venegas, CarolinaBenoist, MarionHärtel, SteffenGonzález Billault, ChristianRodríguez Esteban, José A.Avila, JesúsFarmaciaMicrotubule-associated protein 1B (MAP1B) is prominently expressed during early stages of neuronal development, and it has been implicated in axonal growth and guidance. MAP1B expression is also found in the adult brain in areas of significant synaptic plasticity. Here, we demonstrate that MAP1B is present in dendritic spines, and we describe a decrease in the density of mature dendritic spines in neurons of MAP1B-deficient mice that was accompanied by an increase in the number of immature filopodia-like protrusions. Although these neurons exhibited normal passive membrane properties and action potential firing, AMPA receptor-mediated synaptic currents were significantly diminished. Moreover, we observed a significant decrease in Rac1 activity and an increase in RhoA activity in the postsynaptic densities of adult MAP1B +/- mice when compared with wild type controls. MAP1B +/- fractions also exhibited a decrease in phosphorylated cofilin. Taken together, these results indicate a new and important role for MAP1B in the formation and maturation of dendritic spines, possibly through the regulation of the actin cytoskeleton. This activity of MAP1B could contribute to the regulation of synaptic activity and plasticity in the adult brain. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.ElsevierAmerican Society for Biochemistry and Molecular BiologyDepartamento de Farmacología20112011-11-25research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/717717https://dx.doi.org/10.1074/jbc.M111.271320reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7177172026-06-23T12:46:27Z
dc.title.none.fl_str_mv Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
title Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
spellingShingle Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
Tortosa Binacua, Elena
Farmacia
title_short Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
title_full Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
title_fullStr Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
title_full_unstemmed Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
title_sort Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
dc.creator.none.fl_str_mv Tortosa Binacua, Elena
Montenegro Venegas, Carolina
Benoist, Marion
Härtel, Steffen
González Billault, Christian
Rodríguez Esteban, José A.
Avila, Jesús
author Tortosa Binacua, Elena
author_facet Tortosa Binacua, Elena
Montenegro Venegas, Carolina
Benoist, Marion
Härtel, Steffen
González Billault, Christian
Rodríguez Esteban, José A.
Avila, Jesús
author_role author
author2 Montenegro Venegas, Carolina
Benoist, Marion
Härtel, Steffen
González Billault, Christian
Rodríguez Esteban, José A.
Avila, Jesús
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Farmacología
dc.subject.none.fl_str_mv Farmacia
topic Farmacia
description Microtubule-associated protein 1B (MAP1B) is prominently expressed during early stages of neuronal development, and it has been implicated in axonal growth and guidance. MAP1B expression is also found in the adult brain in areas of significant synaptic plasticity. Here, we demonstrate that MAP1B is present in dendritic spines, and we describe a decrease in the density of mature dendritic spines in neurons of MAP1B-deficient mice that was accompanied by an increase in the number of immature filopodia-like protrusions. Although these neurons exhibited normal passive membrane properties and action potential firing, AMPA receptor-mediated synaptic currents were significantly diminished. Moreover, we observed a significant decrease in Rac1 activity and an increase in RhoA activity in the postsynaptic densities of adult MAP1B +/- mice when compared with wild type controls. MAP1B +/- fractions also exhibited a decrease in phosphorylated cofilin. Taken together, these results indicate a new and important role for MAP1B in the formation and maturation of dendritic spines, possibly through the regulation of the actin cytoskeleton. This activity of MAP1B could contribute to the regulation of synaptic activity and plasticity in the adult brain. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-11-25
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/717717
https://dx.doi.org/10.1074/jbc.M111.271320
url http://hdl.handle.net/10486/717717
https://dx.doi.org/10.1074/jbc.M111.271320
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv Elsevier
American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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