Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis

Endometriosis is a prevalent disease defined by the presence of endometrial tissue outside the uterus. Adenosine triphosphate (ATP), as a proinflammatory molecule, promotes and helps maintain the inflammatory state of endometriosis. Moreover, ATP has a direct influence on the two main symptoms of en...

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Autores: Trapero, Carla, Vidal, August, Fernández-Montolí, Maria-Eulalia, Coroleu, Buenaventura, Tresserra, Francesc, Barri, Pere, Gómez de Aranda, Inmaculada, Sévigny, Jean, Ponce, Jordi, Matias-Guiu, Xavier, Martín-Satué, Mireia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/467714
Acceso en línea:https://doi.org/10.3390/ijms20225532
https://hdl.handle.net/10459.1/467714
Access Level:acceso abierto
Palabra clave:Endometriosis
Endometrium
Uterus
Purinergic signaling
ATP
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spelling Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in EndometriosisTrapero, CarlaVidal, AugustFernández-Montolí, Maria-EulaliaCoroleu, BuenaventuraTresserra, FrancescBarri, PereGómez de Aranda, InmaculadaSévigny, JeanPonce, JordiMatias-Guiu, XavierMartín-Satué, MireiaEndometriosisEndometriumUterusPurinergic signalingATPEndometriosis is a prevalent disease defined by the presence of endometrial tissue outside the uterus. Adenosine triphosphate (ATP), as a proinflammatory molecule, promotes and helps maintain the inflammatory state of endometriosis. Moreover, ATP has a direct influence on the two main symptoms of endometriosis: infertility and pain. Purinergic signaling, the group of biological responses to extracellular nucleotides such as ATP and nucleosides such as adenosine, is involved in the biology of reproduction and is impaired in pathologies with an inflammatory component such as endometriosis. We have previously demonstrated that ectonucleotidases, the enzymes regulating extracellular ATP levels, are active in non-pathological endometria, with hormone-dependent changes in expression throughout the cycle. In the present study we have focused on the expression of ectonucleotidases by means of immunohistochemistry and in situ activity in eutopic and ectopic endometrial tissue of women with endometriosis, and we compared the results with endometria of women without the disease. We have demonstrated that the axis CD39-CD73 is altered in endometriosis, with loss of CD39 and CD73 expression in deep infiltrating endometriosis, the most severe, and most recurring, endometriosis subtype. Our results indicate that this altered expression of ectonucleotidases in endometriosis boosts ATP accumulation in the tissue microenvironment. An important finding is the identification of the nucleotide pyrophophatase/phosphodiesterase 3 (NPP3) as a new histopathological marker of the disease since we have demonstrated its expression in the stroma only in endometriosis, in both eutopic and ectopic tissue. Therefore, targeting the proteins directlMDPI2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/ijms20225532https://hdl.handle.net/10459.1/467714reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a: https://doi.org/10.3390/ijms20225532International Journal of Molecular Sciences, 2019, vol. 20, núm. 22, 5532cc-by (c)The Authors, 2019Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4677142026-06-24T12:42:17Z
dc.title.none.fl_str_mv Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis
title Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis
spellingShingle Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis
Trapero, Carla
Endometriosis
Endometrium
Uterus
Purinergic signaling
ATP
title_short Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis
title_full Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis
title_fullStr Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis
title_full_unstemmed Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis
title_sort Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis
dc.creator.none.fl_str_mv Trapero, Carla
Vidal, August
Fernández-Montolí, Maria-Eulalia
Coroleu, Buenaventura
Tresserra, Francesc
Barri, Pere
Gómez de Aranda, Inmaculada
Sévigny, Jean
Ponce, Jordi
Matias-Guiu, Xavier
Martín-Satué, Mireia
author Trapero, Carla
author_facet Trapero, Carla
Vidal, August
Fernández-Montolí, Maria-Eulalia
Coroleu, Buenaventura
Tresserra, Francesc
Barri, Pere
Gómez de Aranda, Inmaculada
Sévigny, Jean
Ponce, Jordi
Matias-Guiu, Xavier
Martín-Satué, Mireia
author_role author
author2 Vidal, August
Fernández-Montolí, Maria-Eulalia
Coroleu, Buenaventura
Tresserra, Francesc
Barri, Pere
Gómez de Aranda, Inmaculada
Sévigny, Jean
Ponce, Jordi
Matias-Guiu, Xavier
Martín-Satué, Mireia
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Endometriosis
Endometrium
Uterus
Purinergic signaling
ATP
topic Endometriosis
Endometrium
Uterus
Purinergic signaling
ATP
description Endometriosis is a prevalent disease defined by the presence of endometrial tissue outside the uterus. Adenosine triphosphate (ATP), as a proinflammatory molecule, promotes and helps maintain the inflammatory state of endometriosis. Moreover, ATP has a direct influence on the two main symptoms of endometriosis: infertility and pain. Purinergic signaling, the group of biological responses to extracellular nucleotides such as ATP and nucleosides such as adenosine, is involved in the biology of reproduction and is impaired in pathologies with an inflammatory component such as endometriosis. We have previously demonstrated that ectonucleotidases, the enzymes regulating extracellular ATP levels, are active in non-pathological endometria, with hormone-dependent changes in expression throughout the cycle. In the present study we have focused on the expression of ectonucleotidases by means of immunohistochemistry and in situ activity in eutopic and ectopic endometrial tissue of women with endometriosis, and we compared the results with endometria of women without the disease. We have demonstrated that the axis CD39-CD73 is altered in endometriosis, with loss of CD39 and CD73 expression in deep infiltrating endometriosis, the most severe, and most recurring, endometriosis subtype. Our results indicate that this altered expression of ectonucleotidases in endometriosis boosts ATP accumulation in the tissue microenvironment. An important finding is the identification of the nucleotide pyrophophatase/phosphodiesterase 3 (NPP3) as a new histopathological marker of the disease since we have demonstrated its expression in the stroma only in endometriosis, in both eutopic and ectopic tissue. Therefore, targeting the proteins directl
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/ijms20225532
https://hdl.handle.net/10459.1/467714
url https://doi.org/10.3390/ijms20225532
https://hdl.handle.net/10459.1/467714
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms20225532
International Journal of Molecular Sciences, 2019, vol. 20, núm. 22, 5532
dc.rights.none.fl_str_mv cc-by (c)The Authors, 2019
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c)The Authors, 2019
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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