Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection

Among the essential metabolic functions of the liver, in mammals, a role as mediator of systemic and local innate immunity has also been reported. Although the presence of an important leukocyte population in mammalian liver is well documented, the characterization of leukocyte populations in the te...

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Autores: Castro, Rosario, Abós, Beatriz, Pignatelli Garrigos, Jaime, Von Gersdorff Jørgensen, L., Granja, Aitor G., Buchmann, K., Tafalla, Carolina
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/291443
Acceso en línea:http://hdl.handle.net/10261/291443
Access Level:acceso abierto
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spelling Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infectionCastro, RosarioAbós, BeatrizPignatelli Garrigos, JaimeVon Gersdorff Jørgensen, L.Granja, Aitor G.Buchmann, K.Tafalla, CarolinaAmong the essential metabolic functions of the liver, in mammals, a role as mediator of systemic and local innate immunity has also been reported. Although the presence of an important leukocyte population in mammalian liver is well documented, the characterization of leukocyte populations in the teleost liver has been only scarcely addressed. In the current work, we have confirmed the presence of IgM+, IgD+, IgT+, CD8α+, CD3+ cells, and cells expressing major histocompatibility complex (MHC-II) in rainbow trout (Oncorhynchus mykiss) liver by flow cytometry and/or immunohistochemistry analysis. Additionally, the effect of viral hemorrhagic septicemia virus (VHSV) on the liver immune response was assessed. First, we studied the effect of viral intraperitoneal injection on the transcription of a wide selection of immune genes at days 1, 2 and 5 post-infection. These included a group of leukocyte markers genes, pattern recognition receptors (PRRs), chemokines, chemokine receptor genes, and other genes involved in the early immune response and in acute phase reaction. Our results indicate that T lymphocytes play a key role in the initial response to VHSV in the liver, since CD3, CD8, CD4, perforin, Mx and interferon (IFN) transcription levels were up-regulated in response to VHSV. Consequently, flow cytometry analysis of CD8α+ cells in liver and spleen at day 5 post-infection revealed a decrease in the number of CD8α+ cells in the spleen and an increased population in the liver. No differences were found however in the percentages of B lymphocyte (IgM+ or IgD+) populations. In addition, a strong up-regulation in the transcription levels of several PRRs and chemokines was observed from the second day of infection, indicating an important role of these factors in the response of the liver to viral infections. Copyright © 2014 Castro et al.Peer reviewedPublic Library of ScienceAbós, Beatriz [0000-0002-3402-7397]Granja, Aitor G. [0000-0001-7839-3762]Tafalla, Carolina [0000-0002-0860-2976]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/291443reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro de Investigación en Sanidad Animal (CISA)Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2914432026-05-22T06:33:51Z
dc.title.none.fl_str_mv Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection
title Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection
spellingShingle Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection
Castro, Rosario
title_short Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection
title_full Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection
title_fullStr Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection
title_full_unstemmed Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection
title_sort Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection
dc.creator.none.fl_str_mv Castro, Rosario
Abós, Beatriz
Pignatelli Garrigos, Jaime
Von Gersdorff Jørgensen, L.
Granja, Aitor G.
Buchmann, K.
Tafalla, Carolina
author Castro, Rosario
author_facet Castro, Rosario
Abós, Beatriz
Pignatelli Garrigos, Jaime
Von Gersdorff Jørgensen, L.
Granja, Aitor G.
Buchmann, K.
Tafalla, Carolina
author_role author
author2 Abós, Beatriz
Pignatelli Garrigos, Jaime
Von Gersdorff Jørgensen, L.
Granja, Aitor G.
Buchmann, K.
Tafalla, Carolina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Abós, Beatriz [0000-0002-3402-7397]
Granja, Aitor G. [0000-0001-7839-3762]
Tafalla, Carolina [0000-0002-0860-2976]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Among the essential metabolic functions of the liver, in mammals, a role as mediator of systemic and local innate immunity has also been reported. Although the presence of an important leukocyte population in mammalian liver is well documented, the characterization of leukocyte populations in the teleost liver has been only scarcely addressed. In the current work, we have confirmed the presence of IgM+, IgD+, IgT+, CD8α+, CD3+ cells, and cells expressing major histocompatibility complex (MHC-II) in rainbow trout (Oncorhynchus mykiss) liver by flow cytometry and/or immunohistochemistry analysis. Additionally, the effect of viral hemorrhagic septicemia virus (VHSV) on the liver immune response was assessed. First, we studied the effect of viral intraperitoneal injection on the transcription of a wide selection of immune genes at days 1, 2 and 5 post-infection. These included a group of leukocyte markers genes, pattern recognition receptors (PRRs), chemokines, chemokine receptor genes, and other genes involved in the early immune response and in acute phase reaction. Our results indicate that T lymphocytes play a key role in the initial response to VHSV in the liver, since CD3, CD8, CD4, perforin, Mx and interferon (IFN) transcription levels were up-regulated in response to VHSV. Consequently, flow cytometry analysis of CD8α+ cells in liver and spleen at day 5 post-infection revealed a decrease in the number of CD8α+ cells in the spleen and an increased population in the liver. No differences were found however in the percentages of B lymphocyte (IgM+ or IgD+) populations. In addition, a strong up-regulation in the transcription levels of several PRRs and chemokines was observed from the second day of infection, indicating an important role of these factors in the response of the liver to viral infections. Copyright © 2014 Castro et al.
publishDate 2014
dc.date.none.fl_str_mv 2014
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/291443
url http://hdl.handle.net/10261/291443
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro de Investigación en Sanidad Animal (CISA)
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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