Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing

Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffo...

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Autores: Gálvez-Montón, Carolina|||0000-0003-2254-9371, Bragos, Ramon|||0000-0002-1373-1588, Soler Botija, Carolina, Díaz-Güemes, Idoia, Prat-Vidal, Cristina|||0000-0002-5621-1101, Crisóstomo, Verónica|||0000-0003-1740-7029, Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988X, Llucià-Valldeperas, Aida, Bogónez-Franco, Paco|||0000-0002-5364-231X, Perea Gil, Isaac, Rudilla, F.., Bayés-Genís, Antoni|||0000-0002-3044-197X
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:196346
Acceso en línea:https://ddd.uab.cat/record/196346
https://dx.doi.org/urn:doi:10.5966/sctm.2016-0063
Access Level:acceso abierto
Palabra clave:Myocardial infarction
Magnetic resonance imaging
Bioimpedance
Angiogenesis
Progenitor cells
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spelling Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar HealingGálvez-Montón, Carolina|||0000-0003-2254-9371Bragos, Ramon|||0000-0002-1373-1588Soler Botija, CarolinaDíaz-Güemes, IdoiaPrat-Vidal, Cristina|||0000-0002-5621-1101Crisóstomo, Verónica|||0000-0003-1740-7029Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988XLlucià-Valldeperas, AidaBogónez-Franco, Paco|||0000-0002-5364-231XPerea Gil, IsaacRudilla, F..Bayés-Genís, Antoni|||0000-0002-3044-197XMyocardial infarctionMagnetic resonance imagingBioimpedanceAngiogenesisProgenitor cellsCardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffold of decellularized human pericardium, green fluorescent protein-labeled porcine adipose tissue-derived progenitor cells (pATPCs), and a customized-design electrical impedance spectroscopy (EIS) monitoring system. Cardiac function was evaluated noninvasively by using magnetic resonance imaging (MRI). Scar healing was evaluated by using the EIS system within the implanted graft. Additionally, infarct size, fibrosis, and inflammation were explored by histopathology. Upon sacrifice 1 month after the intervention, MRI detected a significant improvement in left ventricular ejection fraction (7.5%64.9% vs. 1.4%63.7%; p = .038) and stroke volume (11.565.9 ml vs. 364.5 ml; p = .019) in EBIG-treated animals. Noninvasive EIS data analysis showed differences in both impedance magnitude ratio (20.02 6 0.04 per day vs. 20.48 6 0.07 per day; p = .002) and phase angle slope (20.18°60.24° per day vs.23.52°60.84° per day; p = .004) in EBIG compared with control animals. Moreover, in EBIG-treated animals, the infarct size was 48% smaller (3.4%60.6% vs. 6.5%61%; p = .015), less inflammation was found by means of CD25+ lymphocytes (0.65 6 0.12 vs. 1.26 6 0.2; p = .006), and a lower collagen I/III ratio was detected (0.4960.06 vs. 1.6660.5; p = .019). An EBIG composed of acellular pericardium refilled with pATPCs significantly reduced infarct size and improved cardiac function in a preclinical model of MI. Noninvasive EIS monitoring was useful for tracking differential scar healing in EBIG-treated animals, which was confirmed by less inflammation and altered collagen deposit. 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/196346https://dx.doi.org/urn:doi:10.5966/sctm.2016-0063reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2011-30067Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2014-59892Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI14/01682open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1963462026-06-06T12:50:31Z
dc.title.none.fl_str_mv Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
title Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
spellingShingle Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
Gálvez-Montón, Carolina|||0000-0003-2254-9371
Myocardial infarction
Magnetic resonance imaging
Bioimpedance
Angiogenesis
Progenitor cells
title_short Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
title_full Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
title_fullStr Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
title_full_unstemmed Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
title_sort Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
dc.creator.none.fl_str_mv Gálvez-Montón, Carolina|||0000-0003-2254-9371
Bragos, Ramon|||0000-0002-1373-1588
Soler Botija, Carolina
Díaz-Güemes, Idoia
Prat-Vidal, Cristina|||0000-0002-5621-1101
Crisóstomo, Verónica|||0000-0003-1740-7029
Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988X
Llucià-Valldeperas, Aida
Bogónez-Franco, Paco|||0000-0002-5364-231X
Perea Gil, Isaac
Rudilla, F..
Bayés-Genís, Antoni|||0000-0002-3044-197X
author Gálvez-Montón, Carolina|||0000-0003-2254-9371
author_facet Gálvez-Montón, Carolina|||0000-0003-2254-9371
Bragos, Ramon|||0000-0002-1373-1588
Soler Botija, Carolina
Díaz-Güemes, Idoia
Prat-Vidal, Cristina|||0000-0002-5621-1101
Crisóstomo, Verónica|||0000-0003-1740-7029
Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988X
Llucià-Valldeperas, Aida
Bogónez-Franco, Paco|||0000-0002-5364-231X
Perea Gil, Isaac
Rudilla, F..
Bayés-Genís, Antoni|||0000-0002-3044-197X
author_role author
author2 Bragos, Ramon|||0000-0002-1373-1588
Soler Botija, Carolina
Díaz-Güemes, Idoia
Prat-Vidal, Cristina|||0000-0002-5621-1101
Crisóstomo, Verónica|||0000-0003-1740-7029
Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988X
Llucià-Valldeperas, Aida
Bogónez-Franco, Paco|||0000-0002-5364-231X
Perea Gil, Isaac
Rudilla, F..
Bayés-Genís, Antoni|||0000-0002-3044-197X
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Myocardial infarction
Magnetic resonance imaging
Bioimpedance
Angiogenesis
Progenitor cells
topic Myocardial infarction
Magnetic resonance imaging
Bioimpedance
Angiogenesis
Progenitor cells
description Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffold of decellularized human pericardium, green fluorescent protein-labeled porcine adipose tissue-derived progenitor cells (pATPCs), and a customized-design electrical impedance spectroscopy (EIS) monitoring system. Cardiac function was evaluated noninvasively by using magnetic resonance imaging (MRI). Scar healing was evaluated by using the EIS system within the implanted graft. Additionally, infarct size, fibrosis, and inflammation were explored by histopathology. Upon sacrifice 1 month after the intervention, MRI detected a significant improvement in left ventricular ejection fraction (7.5%64.9% vs. 1.4%63.7%; p = .038) and stroke volume (11.565.9 ml vs. 364.5 ml; p = .019) in EBIG-treated animals. Noninvasive EIS data analysis showed differences in both impedance magnitude ratio (20.02 6 0.04 per day vs. 20.48 6 0.07 per day; p = .002) and phase angle slope (20.18°60.24° per day vs.23.52°60.84° per day; p = .004) in EBIG compared with control animals. Moreover, in EBIG-treated animals, the infarct size was 48% smaller (3.4%60.6% vs. 6.5%61%; p = .015), less inflammation was found by means of CD25+ lymphocytes (0.65 6 0.12 vs. 1.26 6 0.2; p = .006), and a lower collagen I/III ratio was detected (0.4960.06 vs. 1.6660.5; p = .019). An EBIG composed of acellular pericardium refilled with pATPCs significantly reduced infarct size and improved cardiac function in a preclinical model of MI. Noninvasive EIS monitoring was useful for tracking differential scar healing in EBIG-treated animals, which was confirmed by less inflammation and altered collagen deposit.
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/196346
https://dx.doi.org/urn:doi:10.5966/sctm.2016-0063
url https://ddd.uab.cat/record/196346
https://dx.doi.org/urn:doi:10.5966/sctm.2016-0063
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2011-30067
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2014-59892
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI14/01682
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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