Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection
In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and t...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2024 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositório: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/368554 |
| Acesso em linha: | http://hdl.handle.net/10261/368554 https://api.elsevier.com/content/abstract/scopus_id/85196157952 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Aptasensor Bismuthene nanosheets Nanostructured bioconjugate SARS-CoV-2 aptamer-based tetrahedral DNA nanostructure (TDNapt) Tetrahedral DNA nanostructure (TDN) |
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Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| spellingShingle |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection Enebral-Romero, Estefanía Aptasensor Bismuthene nanosheets Nanostructured bioconjugate SARS-CoV-2 aptamer-based tetrahedral DNA nanostructure (TDNapt) Tetrahedral DNA nanostructure (TDN) |
| title_short |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title_full |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title_fullStr |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title_full_unstemmed |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title_sort |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| dc.creator.none.fl_str_mv |
Enebral-Romero, Estefanía García-Fernández, Daniel Gutiérrez-Gálvez, Laura López-Diego, David Luna, Mónica García-Martín, Adrián Salagre, Elena Michel, Enrique G. Torres, Íñigo Zamora, Félix García-Mendiola, Tania Lorenzo, Encarnación |
| author |
Enebral-Romero, Estefanía |
| author_facet |
Enebral-Romero, Estefanía García-Fernández, Daniel Gutiérrez-Gálvez, Laura López-Diego, David Luna, Mónica García-Martín, Adrián Salagre, Elena Michel, Enrique G. Torres, Íñigo Zamora, Félix García-Mendiola, Tania Lorenzo, Encarnación |
| author_role |
author |
| author2 |
García-Fernández, Daniel Gutiérrez-Gálvez, Laura López-Diego, David Luna, Mónica García-Martín, Adrián Salagre, Elena Michel, Enrique G. Torres, Íñigo Zamora, Félix García-Mendiola, Tania Lorenzo, Encarnación |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Agencia Estatal de Investigación (España) Ministerio de Ciencia, Innovación y Universidades (España) Ministerio de Ciencia e Innovación (España) Comunidad de Madrid European Commission Consejo Superior de Investigaciones Científicas (España) Ministerio de Economía y Competitividad (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Aptasensor Bismuthene nanosheets Nanostructured bioconjugate SARS-CoV-2 aptamer-based tetrahedral DNA nanostructure (TDNapt) Tetrahedral DNA nanostructure (TDN) |
| topic |
Aptasensor Bismuthene nanosheets Nanostructured bioconjugate SARS-CoV-2 aptamer-based tetrahedral DNA nanostructure (TDNapt) Tetrahedral DNA nanostructure (TDN) |
| description |
In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and tetrahedral DNA nanostructures (TDNs) is immobilized on Carbon Screen-Printed Electrodes (CSPE). The TDNs contain on the top vertex an aptamer that specifically binds to the SARS-CoV-2 spike protein, and a thiol group at the three basal vertices to anchor to the FLB. The TDNs are also marked with a redox indicator, Azure A (AA), which allows the direct detection of SARS-CoV-2 spike protein through changes in the current intensity of its electrolysis before and after the biorecognition reaction. The developed sensor can detect SARS-CoV-2 spike protein with a detection limit of 1.74 fg mL-1 directly in nasopharyngeal swab human samples. Therefore, this study offers a new strategy for rapid virus detection since it is versatile enough for different viruses and pathogens. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/368554 https://api.elsevier.com/content/abstract/scopus_id/85196157952 |
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http://hdl.handle.net/10261/368554 https://api.elsevier.com/content/abstract/scopus_id/85196157952 |
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Inglés |
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Inglés |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Bismuthene - Tetrahedral DNA nanobioconjugate for virus detectionEnebral-Romero, EstefaníaGarcía-Fernández, DanielGutiérrez-Gálvez, LauraLópez-Diego, DavidLuna, MónicaGarcía-Martín, AdriánSalagre, ElenaMichel, Enrique G.Torres, ÍñigoZamora, FélixGarcía-Mendiola, TaniaLorenzo, EncarnaciónAptasensorBismuthene nanosheetsNanostructured bioconjugateSARS-CoV-2 aptamer-based tetrahedral DNA nanostructure (TDNapt)Tetrahedral DNA nanostructure (TDN)In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and tetrahedral DNA nanostructures (TDNs) is immobilized on Carbon Screen-Printed Electrodes (CSPE). The TDNs contain on the top vertex an aptamer that specifically binds to the SARS-CoV-2 spike protein, and a thiol group at the three basal vertices to anchor to the FLB. The TDNs are also marked with a redox indicator, Azure A (AA), which allows the direct detection of SARS-CoV-2 spike protein through changes in the current intensity of its electrolysis before and after the biorecognition reaction. The developed sensor can detect SARS-CoV-2 spike protein with a detection limit of 1.74 fg mL-1 directly in nasopharyngeal swab human samples. Therefore, this study offers a new strategy for rapid virus detection since it is versatile enough for different viruses and pathogens.This work has been financially supported by the Spanish Ministry of Economy and Competitiveness (PID2020-116728RB-I00, RED2022-134120-T), Spanish Ministry of Science, Innovation and Universities (TED2021-129738B–I00) and Community of Madrid (REACT-UE NANOCOV-CM, TRANSNANOAVANSENS, S2018/NMT-4349). Estefanía Enebral-Romero thanks the financial support of “Nanotecnología para detección del SARS-CoV-2 y sus variantes. NANOCOV” project and a contract as a predoctoral researcher funded by grant CEX2020-001039-S, supported by MCIN/AEI/10.13039/501100011033. Laura Gutiérrez-Gálvez was supported by a Formación del Profesorado Universitario (FPU) grant from the Spanish Ministry of Universities (FPU19/06309). Daniel García-Fernández and Adrián García-Martín were supported by a Programa Investigo grant from Autonomous Community of Madrid in the framework of the Plan de Recuperación, Transformación y Resiliencia financed by the European Union – NextGenerationEU (09-PIN1-00013.4/2022- 83rd and 66rd place, respectively). The authors acknowledge the support of the European Union (EU) and Horizon 2020 through Instruct Proposal PID: 21859, and the CRIOMECORR project (ESFRI-2019-01-CSIC-16) to the cryoEM CNB-CSIC facility. F. Z. acknowledges financial support from the Spanish Ministry of Science and Innovation, through the “María de Maeztu” Programme for Units of Excellence in R&D (CEX2023-001316-M, PDC2021-120782-C21, and PID2022-138908NB-C31), the “(MAD2D-CM)-UAM” project funded by Comunidad de Madrid, by the Recovery, Transformation and Resilience Plan, and by NextGenerationEU from the European Union. F.Z. also acknowledge support from the European Innovation Council under grant Agreement 101047081 (EVA). EGM acknowledges financial support from the Spanish Ministry of Science and Innovation, through the “María de Maeztu” Programme for Units of Excellence in R&D (CEX2018-000805-M) and through grant PID2021-123295NB-I00. We also acknowledge María U. González help with Fluorescence Microscopy and the service from the MiNa Laboratory at IMN, and funding from CM (project S2018/NMT-4291 TEC2SPACE), MINECO (project CSIC13-4E-1794) and EU (FEDER, FSE).Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Ministerio de Ciencia e Innovación (España)Comunidad de MadridEuropean CommissionConsejo Superior de Investigaciones Científicas (España)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/368554https://api.elsevier.com/content/abstract/scopus_id/85196157952reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116728RB-I00info:eu-repo/grantAgreement/AEI//RED2022-134120-Tinfo:eu-repo/grantAgreement/AEI//TED2021-129738B-I00S2018/NMT-4349/TRANSNANOAVANSENS-CMinfo:eu-repo/grantAgreement/AEI//CEX2020-001039-Sinfo:eu-repo/grantAgreement/AEI//CEX2023-001316-Minfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PDC2021-120782-C21info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-138908NB-C31info:eu-repo/grantAgreement/EC/HE/101047081info:eu-repo/grantAgreement/AEI//CEX2018-000805-Minfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-123295NB-I00S2018/NMT-4291/TEC2SPACEinfo:eu-repo/grantAgreement/MINECO//CSIC13-4E-1794The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.bios.2024.116500https://doi.org/10.1016/j.bios.2024.116500Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3685542026-05-22T06:33:51Z |
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15.812429 |