Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing

Sterilization is a crucial step in the process of developing bioinks for tissue engineering applications. In this work, alginate/gelatin inks were subjected to three sterilization methods: ultraviolet (UV) radiation, filtration (FILT), and autoclaving (AUTO). In addition, to simulate the sterilizati...

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Autores: Carranza Fernandino, Teresa, Zalba Balda, Martín, Barriola Baraibar, Mari Jose, Guerrero Manso, Pedro Manuel, De la Caba Ciriza, María Coro
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/60377
Acceso en línea:http://hdl.handle.net/10810/60377
Access Level:acceso abierto
Palabra clave:inks
three-dimensional printing
constructs
sterilization
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spelling Effect of sterilization processes on alginate/gelatin inks for three-dimensional printingCarranza Fernandino, TeresaZalba Balda, MartínBarriola Baraibar, Mari JoseGuerrero Manso, Pedro ManuelDe la Caba Ciriza, María Coroinksthree-dimensional printingconstructssterilizationSterilization is a crucial step in the process of developing bioinks for tissue engineering applications. In this work, alginate/gelatin inks were subjected to three sterilization methods: ultraviolet (UV) radiation, filtration (FILT), and autoclaving (AUTO). In addition, to simulate the sterilization effect in a real environment, inks were formulated in two different media, specifically, Dulbecco’s Modified Eagle’s Medium (DMEM) and phosphate-buffered saline (PBS). First, rheological tests were performed to evaluate the flow properties of the inks, and we observed that UV samples showed shear thinning behavior, which was favorable for three dimensional (3D) printing. Furthermore, the 3D-printed constructs developed with UV inks showed better shape and size fidelity than those obtained with FILT and AUTO. In order to relate this behavior to the material structure, Fourier transform infrared (FTIR) analysis was carried out and the predominant conformation in protein was determined by deconvolution of the amide I band, which confirmed that the prevalence of a-helix structure was greater for UV samples. This work highlights the relevance of sterilization processes, which are essential for biomedical applications, in the research field of bioinksAuthors thank Basque Government (IT1658-22 and KK2022-00019) for the funding. T.C. also thanks Basque Government for her fellowship (PRE_2021_1_0254).WHIOCE202320232023info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/60377reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://ijb.sg/index.php/int-j-bioprinting/article/view/645info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/(c) 2022 Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License.Atribución 3.0 Españaoai:addi.ehu.eus:10810/603772026-06-18T09:23:17Z
dc.title.none.fl_str_mv Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing
title Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing
spellingShingle Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing
Carranza Fernandino, Teresa
inks
three-dimensional printing
constructs
sterilization
title_short Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing
title_full Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing
title_fullStr Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing
title_full_unstemmed Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing
title_sort Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing
dc.creator.none.fl_str_mv Carranza Fernandino, Teresa
Zalba Balda, Martín
Barriola Baraibar, Mari Jose
Guerrero Manso, Pedro Manuel
De la Caba Ciriza, María Coro
author Carranza Fernandino, Teresa
author_facet Carranza Fernandino, Teresa
Zalba Balda, Martín
Barriola Baraibar, Mari Jose
Guerrero Manso, Pedro Manuel
De la Caba Ciriza, María Coro
author_role author
author2 Zalba Balda, Martín
Barriola Baraibar, Mari Jose
Guerrero Manso, Pedro Manuel
De la Caba Ciriza, María Coro
author2_role author
author
author
author
dc.subject.none.fl_str_mv inks
three-dimensional printing
constructs
sterilization
topic inks
three-dimensional printing
constructs
sterilization
description Sterilization is a crucial step in the process of developing bioinks for tissue engineering applications. In this work, alginate/gelatin inks were subjected to three sterilization methods: ultraviolet (UV) radiation, filtration (FILT), and autoclaving (AUTO). In addition, to simulate the sterilization effect in a real environment, inks were formulated in two different media, specifically, Dulbecco’s Modified Eagle’s Medium (DMEM) and phosphate-buffered saline (PBS). First, rheological tests were performed to evaluate the flow properties of the inks, and we observed that UV samples showed shear thinning behavior, which was favorable for three dimensional (3D) printing. Furthermore, the 3D-printed constructs developed with UV inks showed better shape and size fidelity than those obtained with FILT and AUTO. In order to relate this behavior to the material structure, Fourier transform infrared (FTIR) analysis was carried out and the predominant conformation in protein was determined by deconvolution of the amide I band, which confirmed that the prevalence of a-helix structure was greater for UV samples. This work highlights the relevance of sterilization processes, which are essential for biomedical applications, in the research field of bioinks
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/60377
url http://hdl.handle.net/10810/60377
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://ijb.sg/index.php/int-j-bioprinting/article/view/645
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv WHIOCE
publisher.none.fl_str_mv WHIOCE
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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