Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors

The potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mech...

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Autores: Garcia-Martinez, A, Fuentes-Fayos, AC, Fajardo, C, Lamas, C, Camara, R, Lopez-Munoz, B, Aranda, I, Luque, RM, Pico, A
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
Repositorio:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
OAI Identifier:oai:isabial.fundanetsuite.com:p6699
Acceso en línea:https://isabial.portalinvestigacion.com/publicaciones6699
Access Level:acceso abierto
Palabra clave:pituitary neuroendocrine tumors
corticotroph tumors
miRNA
transcriptional regulation
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spelling Differential Expression of MicroRNAs in Silent and Functioning Corticotroph TumorsGarcia-Martinez, AFuentes-Fayos, ACFajardo, CLamas, CCamara, RLopez-Munoz, BAranda, ILuque, RMPico, Apituitary neuroendocrine tumorscorticotroph tumorsmiRNAtranscriptional regulationThe potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mechanisms of corticotroph tumors (CTs). Accordingly, the expression of miR-375, miR-383, miR-488, miR-200a and miR-103; ofPKA,MAP3K8,MEK,MAPK3,NGFIB,NURR1,PITX1, andSTAT3were analyzed via qRT-PCR in 23 silent and 24 functioning CTs. miR-200a and miR-103 showed significantly higher expression in silent than in functioning CTs, even after eliminating the bias of tumor size, therefore enabling the differentiation between the two variants. Additionally, miR-383 correlated negatively withTBX19in silent CTs, a transcription factor related with the processing ofPOMCthat can participate in the silencing mechanisms of CTs. Finally, the gene expression levels of miR-488, miR-200a, and miR-103 were significantly higher in macroadenomas (functioning and silent) than in microadenomas. The evidence from this study indicates that miRNAs could be involved in the pathophysiology of CTs. The translational implications of these findings suggest that pharmacological treatments specifically targeting these miRNAs could become a promising therapeutic option for these patients.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://isabial.portalinvestigacion.com/publicaciones6699Journal of Clinical MedicineISSN: 20770383reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicanteinstname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)Inglésinfo:eu-repo/semantics/openAccessoai:isabial.fundanetsuite.com:p66992026-06-12T10:20:37Z
dc.title.none.fl_str_mv Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors
title Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors
spellingShingle Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors
Garcia-Martinez, A
pituitary neuroendocrine tumors
corticotroph tumors
miRNA
transcriptional regulation
title_short Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors
title_full Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors
title_fullStr Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors
title_full_unstemmed Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors
title_sort Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors
dc.creator.none.fl_str_mv Garcia-Martinez, A
Fuentes-Fayos, AC
Fajardo, C
Lamas, C
Camara, R
Lopez-Munoz, B
Aranda, I
Luque, RM
Pico, A
author Garcia-Martinez, A
author_facet Garcia-Martinez, A
Fuentes-Fayos, AC
Fajardo, C
Lamas, C
Camara, R
Lopez-Munoz, B
Aranda, I
Luque, RM
Pico, A
author_role author
author2 Fuentes-Fayos, AC
Fajardo, C
Lamas, C
Camara, R
Lopez-Munoz, B
Aranda, I
Luque, RM
Pico, A
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv pituitary neuroendocrine tumors
corticotroph tumors
miRNA
transcriptional regulation
topic pituitary neuroendocrine tumors
corticotroph tumors
miRNA
transcriptional regulation
description The potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mechanisms of corticotroph tumors (CTs). Accordingly, the expression of miR-375, miR-383, miR-488, miR-200a and miR-103; ofPKA,MAP3K8,MEK,MAPK3,NGFIB,NURR1,PITX1, andSTAT3were analyzed via qRT-PCR in 23 silent and 24 functioning CTs. miR-200a and miR-103 showed significantly higher expression in silent than in functioning CTs, even after eliminating the bias of tumor size, therefore enabling the differentiation between the two variants. Additionally, miR-383 correlated negatively withTBX19in silent CTs, a transcription factor related with the processing ofPOMCthat can participate in the silencing mechanisms of CTs. Finally, the gene expression levels of miR-488, miR-200a, and miR-103 were significantly higher in macroadenomas (functioning and silent) than in microadenomas. The evidence from this study indicates that miRNAs could be involved in the pathophysiology of CTs. The translational implications of these findings suggest that pharmacological treatments specifically targeting these miRNAs could become a promising therapeutic option for these patients.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://isabial.portalinvestigacion.com/publicaciones6699
url https://isabial.portalinvestigacion.com/publicaciones6699
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Journal of Clinical Medicine
ISSN: 20770383
reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
instname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
instname_str Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
reponame_str r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
collection r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
repository.name.fl_str_mv
repository.mail.fl_str_mv
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