Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications

Gingival regeneration aims at restoring the architecture and functionality of oral damaged tissue. Different biomaterials or biological materials have been tested for tissue repair, such as platelet concentrates such as PL. In this article, the use of extracellular vesicles (EVs) derived from platel...

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Autores: Antich-Rosselló, Miquel, Munar-Bestard, Marta, Forteza-Genestra, Maria Antònia, Calvo Benito, Javier, Gayà, Antoni, Monjo, Marta, Ramis, Joana Maria
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/18126
Acceso en línea:https://hdl.handle.net/20.500.13003/18126
Access Level:acceso abierto
Palabra clave:Keratinocytes
Extracellular Vesicles
Fibroblasts
Gingiva
Biocompatible Materials
Materiales Biocompatibles
Vesículas Extracelulares
Fibroblastos
Queratinocitos
Encía
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spelling Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal ApplicationsAntich-Rosselló, MiquelMunar-Bestard, MartaForteza-Genestra, Maria AntòniaCalvo Benito, JavierGayà, AntoniMonjo, MartaRamis, Joana MariaKeratinocytesExtracellular VesiclesFibroblastsGingivaBiocompatible MaterialsMateriales BiocompatiblesVesículas ExtracelularesFibroblastosQueratinocitosEncíaGingival regeneration aims at restoring the architecture and functionality of oral damaged tissue. Different biomaterials or biological materials have been tested for tissue repair, such as platelet concentrates such as PL. In this article, the use of extracellular vesicles (EVs) derived from platelet lysate (PL) and their combination with hyaluronic acid biomaterials (HA) in an in vitro wound healing assay is investigated. EVs were isolated by size exclusion chromatography from PL. In addition, HA gels were formulated with PL or EVs. EVs or HA combined with EVs (HA-EVs) were tested in vitro in gingival fibroblasts and keratinocytes for biocompatibility (LDH activity and metabolic activity) and by an in vitro wound-healing assay and gene expression analysis. EVs and EVs-HA treatments were biocompatible in gingival fibroblasts and keratinocytes and showed an increase in wound healing in vitro compared to control. Moreover, changes in gene expression related to extracellular matrix remodeling were observed after the treatment with EVs. EVs can be combined with HA biomaterials, showing good biocompatibility and preserving their activity and functionality. Therefore, platelet-derived EVs could emerge as a new application for periodontal regeneration in combination with biomaterials in order to enhance their clinical use.MPDPI20222022-07-1120222022-07-11research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/18126reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/181262026-06-22T12:44:07Z
dc.title.none.fl_str_mv Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
title Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
spellingShingle Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
Antich-Rosselló, Miquel
Keratinocytes
Extracellular Vesicles
Fibroblasts
Gingiva
Biocompatible Materials
Materiales Biocompatibles
Vesículas Extracelulares
Fibroblastos
Queratinocitos
Encía
title_short Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
title_full Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
title_fullStr Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
title_full_unstemmed Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
title_sort Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications
dc.creator.none.fl_str_mv Antich-Rosselló, Miquel
Munar-Bestard, Marta
Forteza-Genestra, Maria Antònia
Calvo Benito, Javier
Gayà, Antoni
Monjo, Marta
Ramis, Joana Maria
author Antich-Rosselló, Miquel
author_facet Antich-Rosselló, Miquel
Munar-Bestard, Marta
Forteza-Genestra, Maria Antònia
Calvo Benito, Javier
Gayà, Antoni
Monjo, Marta
Ramis, Joana Maria
author_role author
author2 Munar-Bestard, Marta
Forteza-Genestra, Maria Antònia
Calvo Benito, Javier
Gayà, Antoni
Monjo, Marta
Ramis, Joana Maria
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Keratinocytes
Extracellular Vesicles
Fibroblasts
Gingiva
Biocompatible Materials
Materiales Biocompatibles
Vesículas Extracelulares
Fibroblastos
Queratinocitos
Encía
topic Keratinocytes
Extracellular Vesicles
Fibroblasts
Gingiva
Biocompatible Materials
Materiales Biocompatibles
Vesículas Extracelulares
Fibroblastos
Queratinocitos
Encía
description Gingival regeneration aims at restoring the architecture and functionality of oral damaged tissue. Different biomaterials or biological materials have been tested for tissue repair, such as platelet concentrates such as PL. In this article, the use of extracellular vesicles (EVs) derived from platelet lysate (PL) and their combination with hyaluronic acid biomaterials (HA) in an in vitro wound healing assay is investigated. EVs were isolated by size exclusion chromatography from PL. In addition, HA gels were formulated with PL or EVs. EVs or HA combined with EVs (HA-EVs) were tested in vitro in gingival fibroblasts and keratinocytes for biocompatibility (LDH activity and metabolic activity) and by an in vitro wound-healing assay and gene expression analysis. EVs and EVs-HA treatments were biocompatible in gingival fibroblasts and keratinocytes and showed an increase in wound healing in vitro compared to control. Moreover, changes in gene expression related to extracellular matrix remodeling were observed after the treatment with EVs. EVs can be combined with HA biomaterials, showing good biocompatibility and preserving their activity and functionality. Therefore, platelet-derived EVs could emerge as a new application for periodontal regeneration in combination with biomaterials in order to enhance their clinical use.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-07-11
2022
2022-07-11
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/18126
url https://hdl.handle.net/20.500.13003/18126
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MPDPI
publisher.none.fl_str_mv MPDPI
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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