The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development

The heterocyst is the site of nitrogen fixation in aerobically grown cultures of some filamentous cyanobacteria. Heterocyst development in Anabaena sp. strain PCC 7120 is dependent on the global nitrogen regulator NtcA and requires, among others, the products of the herr and hetC genes. Expression o...

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Detalles Bibliográficos
Autores: Muro-Pastor, Alicia M., Valladares, Ana, Flores, Enrique, Herrero, Antonia
Tipo de recurso: artículo
Fecha de publicación:1999
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/111853
Acceso en línea:http://hdl.handle.net/10261/111853
Access Level:acceso abierto
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spelling The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst developmentMuro-Pastor, Alicia M.Valladares, AnaFlores, EnriqueHerrero, AntoniaThe heterocyst is the site of nitrogen fixation in aerobically grown cultures of some filamentous cyanobacteria. Heterocyst development in Anabaena sp. strain PCC 7120 is dependent on the global nitrogen regulator NtcA and requires, among others, the products of the herr and hetC genes. Expression of hetC, tested by RNA-DNA hybridization, was impaired in an ntcA mutant. A nitrogen-regulated, NtcA-dependent putative transcription start point was localized at nucleotide -571 with respect to the hetC translational start. Sequences upstream from this transcription start point exhibit the structure of the canonical cyanobacterial promoter activated by NtcA, and purified NtcA protein specifically bound to a DNA fragment containing this promoter. Activation of expression of hetC during heterocyst development appears thus to be directly operated by NtcA. NtcA-mediated activation of herr expression was not impaired in a hetC mutant, indicating that HetC is not an NtcA-dependent element required for herr induction.Peer ReviewedAmerican Society for Microbiology2015201519992015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/111853reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1118532026-05-22T06:33:51Z
dc.title.none.fl_str_mv The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development
title The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development
spellingShingle The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development
Muro-Pastor, Alicia M.
title_short The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development
title_full The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development
title_fullStr The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development
title_full_unstemmed The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development
title_sort The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development
dc.creator.none.fl_str_mv Muro-Pastor, Alicia M.
Valladares, Ana
Flores, Enrique
Herrero, Antonia
author Muro-Pastor, Alicia M.
author_facet Muro-Pastor, Alicia M.
Valladares, Ana
Flores, Enrique
Herrero, Antonia
author_role author
author2 Valladares, Ana
Flores, Enrique
Herrero, Antonia
author2_role author
author
author
description The heterocyst is the site of nitrogen fixation in aerobically grown cultures of some filamentous cyanobacteria. Heterocyst development in Anabaena sp. strain PCC 7120 is dependent on the global nitrogen regulator NtcA and requires, among others, the products of the herr and hetC genes. Expression of hetC, tested by RNA-DNA hybridization, was impaired in an ntcA mutant. A nitrogen-regulated, NtcA-dependent putative transcription start point was localized at nucleotide -571 with respect to the hetC translational start. Sequences upstream from this transcription start point exhibit the structure of the canonical cyanobacterial promoter activated by NtcA, and purified NtcA protein specifically bound to a DNA fragment containing this promoter. Activation of expression of hetC during heterocyst development appears thus to be directly operated by NtcA. NtcA-mediated activation of herr expression was not impaired in a hetC mutant, indicating that HetC is not an NtcA-dependent element required for herr induction.
publishDate 1999
dc.date.none.fl_str_mv 1999
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/111853
url http://hdl.handle.net/10261/111853
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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