Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure
In this work, the determination of 2,4-, 2,5- and 2,6-dinitrophenols and the identification of some of their metabolites in human urine and saliva is proposed. A three phase hollow fiber based liquid phase microextraction prior to ultra-high performance liquid chromatography coupled to quadrupole ti...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2021 |
| País: | España |
| Recursos: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/153302 |
| Acesso em linha: | https://hdl.handle.net/11441/153302 https://doi.org/10.1016/j.microc.2021.106193 |
| Access Level: | acceso abierto |
| Palavra-chave: | Hollow fiber liquid phase microextraction HF-LPME Liquid chromatography quadrupole time-of-flight Dinitrophenols Human urine Human saliva |
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Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposureKazakova, JuliaVillar Navarro, MercedesPérez Bernal, Juan LuisRamos Payán, María DoloresBello López, Miguel ÁngelFernández Torres, RutHollow fiber liquid phase microextractionHF-LPMELiquid chromatography quadrupole time-of-flightDinitrophenolsHuman urineHuman salivaIn this work, the determination of 2,4-, 2,5- and 2,6-dinitrophenols and the identification of some of their metabolites in human urine and saliva is proposed. A three phase hollow fiber based liquid phase microextraction prior to ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry allowed low detection and quantitation limits of the target analytes, as well as the investigation and tentatively identification of some metabolites by accurate mass full-spectrum measurements. The chromatographic separation was accomplished on an Acquity BEH C18 column (50 mm × 2.1 mm i.d., 1.7 μm particle size) at 25 ºC using water and acetonitrile (with 0.1 % (v/v) formic acid) 20:80 v/v as mobile phase, at a flow rate of 0.5 mL/min in isocratic elution mode for 5 min. Hollow fiber liquid phase microextraction was achieved at donor phase pH 2, acceptor phase pH 13 and dihexylether as supported liquid membrane. Under the optimal conditions, detection limits for 2,4-, 2,5- and 2,6-dinitrophenol, respectively, were 0.18 μg·L-1, 0.38 μg·L-1 and 0.14 μg·L-1 in urine samples and 0.32 μg·L-1, 0.67 μg·L-1 and 0.24 μg·L-1 in saliva samples. The proposed methodology was applied on urine and saliva samples from laboratory staff likely to be or not occupationally exposed to dinitrophenols, finding quantitative levels of 2,4- and 2,6-dinitrophenol and identifying some metabolites previously reported in literature.Ministerio de Economía y Competitividad (MINECO). España CTM2015-67902-C-1-PMinisterio de Ciencia e Innovación (MICIN). España GC2018-096608-B-C22European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) GC2018-096608-B-C22Elsevier Inc.Química AnalíticaMinisterio de Economía y Competitividad (MINECO). EspañaMinisterio de Ciencia e Innovación (MICIN). EspañaEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/153302https://doi.org/10.1016/j.microc.2021.106193reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMicrochemical journal, 166, 106193.CTM2015-67902-C-1-PGC2018-096608-B-C22https://doi.org/10.1016/j.microc.2021.106193info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1533022026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure |
| title |
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure |
| spellingShingle |
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure Kazakova, Julia Hollow fiber liquid phase microextraction HF-LPME Liquid chromatography quadrupole time-of-flight Dinitrophenols Human urine Human saliva |
| title_short |
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure |
| title_full |
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure |
| title_fullStr |
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure |
| title_full_unstemmed |
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure |
| title_sort |
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure |
| dc.creator.none.fl_str_mv |
Kazakova, Julia Villar Navarro, Mercedes Pérez Bernal, Juan Luis Ramos Payán, María Dolores Bello López, Miguel Ángel Fernández Torres, Rut |
| author |
Kazakova, Julia |
| author_facet |
Kazakova, Julia Villar Navarro, Mercedes Pérez Bernal, Juan Luis Ramos Payán, María Dolores Bello López, Miguel Ángel Fernández Torres, Rut |
| author_role |
author |
| author2 |
Villar Navarro, Mercedes Pérez Bernal, Juan Luis Ramos Payán, María Dolores Bello López, Miguel Ángel Fernández Torres, Rut |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Química Analítica Ministerio de Economía y Competitividad (MINECO). España Ministerio de Ciencia e Innovación (MICIN). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
| dc.subject.none.fl_str_mv |
Hollow fiber liquid phase microextraction HF-LPME Liquid chromatography quadrupole time-of-flight Dinitrophenols Human urine Human saliva |
| topic |
Hollow fiber liquid phase microextraction HF-LPME Liquid chromatography quadrupole time-of-flight Dinitrophenols Human urine Human saliva |
| description |
In this work, the determination of 2,4-, 2,5- and 2,6-dinitrophenols and the identification of some of their metabolites in human urine and saliva is proposed. A three phase hollow fiber based liquid phase microextraction prior to ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry allowed low detection and quantitation limits of the target analytes, as well as the investigation and tentatively identification of some metabolites by accurate mass full-spectrum measurements. The chromatographic separation was accomplished on an Acquity BEH C18 column (50 mm × 2.1 mm i.d., 1.7 μm particle size) at 25 ºC using water and acetonitrile (with 0.1 % (v/v) formic acid) 20:80 v/v as mobile phase, at a flow rate of 0.5 mL/min in isocratic elution mode for 5 min. Hollow fiber liquid phase microextraction was achieved at donor phase pH 2, acceptor phase pH 13 and dihexylether as supported liquid membrane. Under the optimal conditions, detection limits for 2,4-, 2,5- and 2,6-dinitrophenol, respectively, were 0.18 μg·L-1, 0.38 μg·L-1 and 0.14 μg·L-1 in urine samples and 0.32 μg·L-1, 0.67 μg·L-1 and 0.24 μg·L-1 in saliva samples. The proposed methodology was applied on urine and saliva samples from laboratory staff likely to be or not occupationally exposed to dinitrophenols, finding quantitative levels of 2,4- and 2,6-dinitrophenol and identifying some metabolites previously reported in literature. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
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article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/153302 https://doi.org/10.1016/j.microc.2021.106193 |
| url |
https://hdl.handle.net/11441/153302 https://doi.org/10.1016/j.microc.2021.106193 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Microchemical journal, 166, 106193. CTM2015-67902-C-1-P GC2018-096608-B-C22 https://doi.org/10.1016/j.microc.2021.106193 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier Inc. |
| publisher.none.fl_str_mv |
Elsevier Inc. |
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reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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