Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture

Bacterial infection remains a significant problem associated with orthopaedic surgeries leading to surgical site infection (SSI). This unmet medical need can become an even greater complication when surgery is due to malignant bone tumor. In the present study, we evaluated in vitro titanium (Ti) imp...

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Autores: Piñera Avellaneda, David|||0000-0001-9383-7309, Buxadera Palomero, Judit|||0000-0003-0897-2093, Manero Planella, José María|||0000-0002-1673-4389, Ginebra Molins, Maria Pau|||0000-0002-4700-5621, Rupérez de Gracia, Elisa|||0000-0001-8845-512X
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/409084
Acesso em linha:https://hdl.handle.net/2117/409084
https://dx.doi.org/10.1016/j.actbio.2024.04.019
Access Level:acceso abierto
Palavra-chave:Silver
Gallium
Titanium
Bone regeneration
Bone resorption
Antibacterial
Plata
Gal·li
Titani
Ossos -- Regeneració
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
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spelling Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culturePiñera Avellaneda, David|||0000-0001-9383-7309Buxadera Palomero, Judit|||0000-0003-0897-2093Manero Planella, José María|||0000-0002-1673-4389Ginebra Molins, Maria Pau|||0000-0002-4700-5621Rupérez de Gracia, Elisa|||0000-0001-8845-512XSilverGalliumTitaniumBone regenerationSilverGalliumTitaniumBone resorptionAntibacterialPlataGal·liTitaniOssos -- RegeneracióÀrees temàtiques de la UPC::Enginyeria biomèdica::BiomaterialsBacterial infection remains a significant problem associated with orthopaedic surgeries leading to surgical site infection (SSI). This unmet medical need can become an even greater complication when surgery is due to malignant bone tumor. In the present study, we evaluated in vitro titanium (Ti) implants subjected to gallium (Ga) and silver (Ag)-doped thermochemical treatment as strategy to prevent SSI and improve osteointegration in bone defects caused by diseases such as osteoporosis, bone tumor, or bone metastasis. Firstly, as Ga has been reported to be an osteoinductive and anti-resorptive agent, its performance in the mixture was proved by studying human mesenchymal stem cells (hMSC) and pre-osteoclasts (RAW264.7) behaviour. Then, the antibacterial potential provided by Ag was assessed by resembling “The Race for the Surface” between hMSC and Pseudomonas aeruginosa in two co-culture methods. Moreover, the presence of quorum sensing molecules in the co-culture was evaluated. The results highlighted the suitability of the mixture to induce osteodifferentiation and reduce osteoclastogenesis in vitro. Furthermore, the GaAg surface promoted strong survival rate and retained osteoinduction potential of hMSCs even after bacterial inoculation. Therefore, GaAg-modified titanium may be an ideal candidate to repair bone defects caused by excessive bone resorption, in addition to preventing SSI.Peer ReviewedElsevier20242024-04-1420242024-05-31journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/409084https://dx.doi.org/10.1016/j.actbio.2024.04.019reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengAgencia Estatal de Investigación http://doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-098075-B-C21 RECUBRIMIENTOS DE BIOMATERIALES DE DOBLE ACCION PARA INFECCIONES BACTERIANAS E INTEGRACION DE TEJIDOSAgencia Estatal de Investigación http://doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-125150OB-I00 DESARROLLO DE SCAFFOLDS METALICOS PERMANENTES Y REABSORBIBLES A MEDIDA IMPRESOS EN 3D CON HIDROGEL BIOFUNCIONALIZADO PARA LA REGENERACION OSEAopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/4090842026-05-27T15:37:01Z
dc.title.none.fl_str_mv Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
title Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
spellingShingle Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
Piñera Avellaneda, David|||0000-0001-9383-7309
Silver
Gallium
Titanium
Bone regeneration
Silver
Gallium
Titanium
Bone resorption
Antibacterial
Plata
Gal·li
Titani
Ossos -- Regeneració
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
title_short Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
title_full Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
title_fullStr Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
title_full_unstemmed Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
title_sort Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
dc.creator.none.fl_str_mv Piñera Avellaneda, David|||0000-0001-9383-7309
Buxadera Palomero, Judit|||0000-0003-0897-2093
Manero Planella, José María|||0000-0002-1673-4389
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Rupérez de Gracia, Elisa|||0000-0001-8845-512X
author Piñera Avellaneda, David|||0000-0001-9383-7309
author_facet Piñera Avellaneda, David|||0000-0001-9383-7309
Buxadera Palomero, Judit|||0000-0003-0897-2093
Manero Planella, José María|||0000-0002-1673-4389
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Rupérez de Gracia, Elisa|||0000-0001-8845-512X
author_role author
author2 Buxadera Palomero, Judit|||0000-0003-0897-2093
Manero Planella, José María|||0000-0002-1673-4389
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Rupérez de Gracia, Elisa|||0000-0001-8845-512X
author2_role author
author
author
author
dc.subject.none.fl_str_mv Silver
Gallium
Titanium
Bone regeneration
Silver
Gallium
Titanium
Bone resorption
Antibacterial
Plata
Gal·li
Titani
Ossos -- Regeneració
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
topic Silver
Gallium
Titanium
Bone regeneration
Silver
Gallium
Titanium
Bone resorption
Antibacterial
Plata
Gal·li
Titani
Ossos -- Regeneració
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
description Bacterial infection remains a significant problem associated with orthopaedic surgeries leading to surgical site infection (SSI). This unmet medical need can become an even greater complication when surgery is due to malignant bone tumor. In the present study, we evaluated in vitro titanium (Ti) implants subjected to gallium (Ga) and silver (Ag)-doped thermochemical treatment as strategy to prevent SSI and improve osteointegration in bone defects caused by diseases such as osteoporosis, bone tumor, or bone metastasis. Firstly, as Ga has been reported to be an osteoinductive and anti-resorptive agent, its performance in the mixture was proved by studying human mesenchymal stem cells (hMSC) and pre-osteoclasts (RAW264.7) behaviour. Then, the antibacterial potential provided by Ag was assessed by resembling “The Race for the Surface” between hMSC and Pseudomonas aeruginosa in two co-culture methods. Moreover, the presence of quorum sensing molecules in the co-culture was evaluated. The results highlighted the suitability of the mixture to induce osteodifferentiation and reduce osteoclastogenesis in vitro. Furthermore, the GaAg surface promoted strong survival rate and retained osteoinduction potential of hMSCs even after bacterial inoculation. Therefore, GaAg-modified titanium may be an ideal candidate to repair bone defects caused by excessive bone resorption, in addition to preventing SSI.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-04-14
2024
2024-05-31
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/409084
https://dx.doi.org/10.1016/j.actbio.2024.04.019
url https://hdl.handle.net/2117/409084
https://dx.doi.org/10.1016/j.actbio.2024.04.019
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-098075-B-C21 RECUBRIMIENTOS DE BIOMATERIALES DE DOBLE ACCION PARA INFECCIONES BACTERIANAS E INTEGRACION DE TEJIDOS
Agencia Estatal de Investigación http://doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-125150OB-I00 DESARROLLO DE SCAFFOLDS METALICOS PERMANENTES Y REABSORBIBLES A MEDIDA IMPRESOS EN 3D CON HIDROGEL BIOFUNCIONALIZADO PARA LA REGENERACION OSEA
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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