Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays

sthma is a heterogeneous disease with several clinical phenotypes and molecular endotypes. However, the specific connection between asthma phenotypes and the underlying pathological features is difficult to explain. Thus, the overall aim of this thesis was to search for biomarkers associated with rh...

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Detalles Bibliográficos
Autor: Nieto Fontarigo, Juan José
Tipo de recurso: tesis doctoral
Fecha de publicación:2019
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/19108
Acceso en línea:http://hdl.handle.net/10347/19108
Access Level:acceso abierto
Palabra clave:Materias::Investigación::23 Química::2302 Bioquímica::230227 Proteínas
Materias::Investigación::24 Ciencias de la vida::2412 Inmunología::241299 asma
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spelling Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassaysNieto Fontarigo, Juan JoséMaterias::Investigación::23 Química::2302 Bioquímica::230227 ProteínasMaterias::Investigación::24 Ciencias de la vida::2412 Inmunología::241299 asmasthma is a heterogeneous disease with several clinical phenotypes and molecular endotypes. However, the specific connection between asthma phenotypes and the underlying pathological features is difficult to explain. Thus, the overall aim of this thesis was to search for biomarkers associated with rhinitis and different phenotypes (allergic and non-allergic) and severities (intermittent-mild and moderate-severe) of asthma. To achieve this aim, we studied the role of the immune system through the analysis of certain biomarkers previously related to this disease: CD14 (innate immune system) and CD26/CD126 (adaptive immune system). In addition, in the second part of the present thesis, a prospective, non-target proteomic study aimed to identify new biomarkers associated with different phenotypes of this disease was also performed. This study consisted of the analysis of low abundance serum proteome through iTRAQ-LC-MS/MS and allowed us the identification of several potential biomarkers for allergic (e.g., IGFALS, protein AMBP, or HSPG2) and non-allergic asthma (e.g., CFI), as well as disease severity (e.g., IGFALS).Salgado Castro, Francisco JavierNogueira Álvarez, MontserratUniversidade de Santiago de Compostela. Centro Internacional de Estudos de Doutoramento e Avanzados (CIEDUS)Universidade de Santiago de Compostela. Escola de Doutoramento Internacional en Ciencias da Saúde20192019-01-0120192019-01-01doctoral thesishttp://purl.org/coar/resource_type/c_db06info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10347/19108reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/191082026-06-15T12:47:27Z
dc.title.none.fl_str_mv Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays
title Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays
spellingShingle Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays
Nieto Fontarigo, Juan José
Materias::Investigación::23 Química::2302 Bioquímica::230227 Proteínas
Materias::Investigación::24 Ciencias de la vida::2412 Inmunología::241299 asma
title_short Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays
title_full Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays
title_fullStr Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays
title_full_unstemmed Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays
title_sort Search for biomarkers related to rhinitis and different asthma phenotypes by serum proteomics and immunoassays
dc.creator.none.fl_str_mv Nieto Fontarigo, Juan José
author Nieto Fontarigo, Juan José
author_facet Nieto Fontarigo, Juan José
author_role author
dc.contributor.none.fl_str_mv Salgado Castro, Francisco Javier
Nogueira Álvarez, Montserrat
Universidade de Santiago de Compostela. Centro Internacional de Estudos de Doutoramento e Avanzados (CIEDUS)
Universidade de Santiago de Compostela. Escola de Doutoramento Internacional en Ciencias da Saúde
dc.subject.none.fl_str_mv Materias::Investigación::23 Química::2302 Bioquímica::230227 Proteínas
Materias::Investigación::24 Ciencias de la vida::2412 Inmunología::241299 asma
topic Materias::Investigación::23 Química::2302 Bioquímica::230227 Proteínas
Materias::Investigación::24 Ciencias de la vida::2412 Inmunología::241299 asma
description sthma is a heterogeneous disease with several clinical phenotypes and molecular endotypes. However, the specific connection between asthma phenotypes and the underlying pathological features is difficult to explain. Thus, the overall aim of this thesis was to search for biomarkers associated with rhinitis and different phenotypes (allergic and non-allergic) and severities (intermittent-mild and moderate-severe) of asthma. To achieve this aim, we studied the role of the immune system through the analysis of certain biomarkers previously related to this disease: CD14 (innate immune system) and CD26/CD126 (adaptive immune system). In addition, in the second part of the present thesis, a prospective, non-target proteomic study aimed to identify new biomarkers associated with different phenotypes of this disease was also performed. This study consisted of the analysis of low abundance serum proteome through iTRAQ-LC-MS/MS and allowed us the identification of several potential biomarkers for allergic (e.g., IGFALS, protein AMBP, or HSPG2) and non-allergic asthma (e.g., CFI), as well as disease severity (e.g., IGFALS).
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10347/19108
url http://hdl.handle.net/10347/19108
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
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