Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA

The major challenges in diagnosing small ruminant lentivirus (SRLV) infection include early detection and genotyping of strains of epidemiological interest. A longitudinal study was carried out in Rasa Aragonesa sheep experimentally infected with viral strains of genotypes A or B from Spanish neurol...

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Autores: Sanjosé, Leticia, Crespo Otano, Helena, Glaría Ezquer, Idoia, Andrés Cara, Damián de, Reina Arias, Ramsés
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/38131
Acceso en línea:https://hdl.handle.net/2454/38131
Access Level:acceso abierto
Palabra clave:Small ruminant lentivirus
Genotype
Serology
Peptide ELISA
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spelling Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISASanjosé, LeticiaCrespo Otano, HelenaGlaría Ezquer, IdoiaAndrés Cara, Damián deReina Arias, RamsésSmall ruminant lentivirusGenotypeSerologyPeptide ELISAThe major challenges in diagnosing small ruminant lentivirus (SRLV) infection include early detection and genotyping of strains of epidemiological interest. A longitudinal study was carried out in Rasa Aragonesa sheep experimentally infected with viral strains of genotypes A or B from Spanish neurological and arthritic SRLV outbreaks, respectively. Sera were tested with two commercial ELISAs, three based on specific peptides and a novel combined peptide ELISA. Three different PCR assays were used to further assess infection status. The kinetics of anti-viral antibody responses were variable, with early diagnosis dependent on the type of ELISA used. Peptide epitopes of SRLV genotypes A and B combined in the same ELISA well enhanced the overall detection rate, whereas single peptides were useful for genotyping the infecting strain (A vs. B). The results of the study suggest that a combined peptide ELISA can be used for serological diagnosis of SRLV infection, with single peptide ELISAs useful for subsequent serotyping.Funded by CICYT (AGL2010-22341-C04-01 and AGL2013-49137-C3-1R) and Navarra’s Government (IIQ010449.RI1 and IIQ14064.RI1). L. Sanjosé was a FPI-fellow of the Spanish MINECO and R. Reina had a contract of the Public University of Navarra.ElsevierIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako InstitutuaGobierno de Navarra / Nafarroako GobernuaUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoa2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2454/38131reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MINECO//AGL2013-49137-C3-1-R© 2015 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/381312026-06-17T12:41:47Z
dc.title.none.fl_str_mv Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA
title Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA
spellingShingle Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA
Sanjosé, Leticia
Small ruminant lentivirus
Genotype
Serology
Peptide ELISA
title_short Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA
title_full Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA
title_fullStr Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA
title_full_unstemmed Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA
title_sort Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA
dc.creator.none.fl_str_mv Sanjosé, Leticia
Crespo Otano, Helena
Glaría Ezquer, Idoia
Andrés Cara, Damián de
Reina Arias, Ramsés
author Sanjosé, Leticia
author_facet Sanjosé, Leticia
Crespo Otano, Helena
Glaría Ezquer, Idoia
Andrés Cara, Damián de
Reina Arias, Ramsés
author_role author
author2 Crespo Otano, Helena
Glaría Ezquer, Idoia
Andrés Cara, Damián de
Reina Arias, Ramsés
author2_role author
author
author
author
dc.contributor.none.fl_str_mv IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Gobierno de Navarra / Nafarroako Gobernua
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
dc.subject.none.fl_str_mv Small ruminant lentivirus
Genotype
Serology
Peptide ELISA
topic Small ruminant lentivirus
Genotype
Serology
Peptide ELISA
description The major challenges in diagnosing small ruminant lentivirus (SRLV) infection include early detection and genotyping of strains of epidemiological interest. A longitudinal study was carried out in Rasa Aragonesa sheep experimentally infected with viral strains of genotypes A or B from Spanish neurological and arthritic SRLV outbreaks, respectively. Sera were tested with two commercial ELISAs, three based on specific peptides and a novel combined peptide ELISA. Three different PCR assays were used to further assess infection status. The kinetics of anti-viral antibody responses were variable, with early diagnosis dependent on the type of ELISA used. Peptide epitopes of SRLV genotypes A and B combined in the same ELISA well enhanced the overall detection rate, whereas single peptides were useful for genotyping the infecting strain (A vs. B). The results of the study suggest that a combined peptide ELISA can be used for serological diagnosis of SRLV infection, with single peptide ELISAs useful for subsequent serotyping.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/38131
url https://hdl.handle.net/2454/38131
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//AGL2013-49137-C3-1-R
dc.rights.none.fl_str_mv © 2015 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0.
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2015 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0.
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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