Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep

[EN]Background/Aims: Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study w...

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Autores: Balseiro Morales, Ana María, Altuzarra, Raúl, Vidal, Enric, Moll, Xavier, Espada, Yvonne, Sevilla, Iker A., Domingo, Mariano, Garrido, Joseba M., Juste, Ramón A., Prieto, Miguel, Pérez de Val, Bernat
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/19718
Acesso em linha:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180546
https://hdl.handle.net/10612/19718
Access Level:acceso abierto
Palavra-chave:Sanidad animal
Veterinaria
BCG vaccine
Mycobacterium bovis
Vacunas (Veterinaria)
Ganado ovino
3109 Ciencias Veterinarias
3104.07 Ovinos
2412.10 Vacunas
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oai_identifier_str oai:buleria.unileon.es:10612/19718
network_acronym_str ES
network_name_str España
repository_id_str
spelling Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheepBalseiro Morales, Ana MaríaAltuzarra, RaúlVidal, EnricMoll, XavierEspada, YvonneSevilla, Iker A.Domingo, MarianoGarrido, Joseba M.Juste, Ramón A.Prieto, MiguelPérez de Val, BernatSanidad animalVeterinariaBCG vaccineMycobacterium bovisVacunas (Veterinaria)Ganado ovino3109 Ciencias Veterinarias3104.07 Ovinos2412.10 Vacunas[EN]Background/Aims: Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study were to develop an experimental TB infection model in sheep with a Mycobacterium caprae field strain isolated from a tuberculous diseased ewe, and to use this to evaluate the safety and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. Methods: Eighteen 2 month-old lambs were experimentally challenged with M. caprae by the endotracheal route (1.5 × 103 CFU). They were separated per treatment group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n = 6). Clinical, immunological, pathological and bacteriological parameters of infection were measured. Results: All lambs were successfully infected and developed gross TB lesions in the respiratory system. The BCG vaccine conferred considerable protection against experimental TB in lambs, as measured by a reduction of the gross lesion volumes and bacterial load. However, HIMB vaccinated animals did not show protection. Conclusions: This study proposes a reliable new experimental model for a better understanding of tuberculosis in sheep. BCG vaccination offers an effective prospect for controlling the disease. Moreover alternative doses and/or routes of administration should be considered to evaluate the efficacy of the HIMB vaccine candidate.SIWe are grateful to Albert Canturri (UAB), Maite Martín, Zoraida Cervera and the staff of the BSL-3 Unit of IRTA-CReSA for their technical support. We also thank Kevin P. Dalton for critically reviewing the manuscript. IRTA is supported by CERCA Programme / Generalitat de Catalunya.Public Library of ScienceSanidad AnimalFacultad de Veterinaria2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180546https://hdl.handle.net/10612/19718reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2014-00002-C02-01http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/197182026-06-24T12:43:27Z
dc.title.none.fl_str_mv Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
title Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
spellingShingle Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
Balseiro Morales, Ana María
Sanidad animal
Veterinaria
BCG vaccine
Mycobacterium bovis
Vacunas (Veterinaria)
Ganado ovino
3109 Ciencias Veterinarias
3104.07 Ovinos
2412.10 Vacunas
title_short Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
title_full Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
title_fullStr Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
title_full_unstemmed Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
title_sort Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
dc.creator.none.fl_str_mv Balseiro Morales, Ana María
Altuzarra, Raúl
Vidal, Enric
Moll, Xavier
Espada, Yvonne
Sevilla, Iker A.
Domingo, Mariano
Garrido, Joseba M.
Juste, Ramón A.
Prieto, Miguel
Pérez de Val, Bernat
author Balseiro Morales, Ana María
author_facet Balseiro Morales, Ana María
Altuzarra, Raúl
Vidal, Enric
Moll, Xavier
Espada, Yvonne
Sevilla, Iker A.
Domingo, Mariano
Garrido, Joseba M.
Juste, Ramón A.
Prieto, Miguel
Pérez de Val, Bernat
author_role author
author2 Altuzarra, Raúl
Vidal, Enric
Moll, Xavier
Espada, Yvonne
Sevilla, Iker A.
Domingo, Mariano
Garrido, Joseba M.
Juste, Ramón A.
Prieto, Miguel
Pérez de Val, Bernat
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
Veterinaria
BCG vaccine
Mycobacterium bovis
Vacunas (Veterinaria)
Ganado ovino
3109 Ciencias Veterinarias
3104.07 Ovinos
2412.10 Vacunas
topic Sanidad animal
Veterinaria
BCG vaccine
Mycobacterium bovis
Vacunas (Veterinaria)
Ganado ovino
3109 Ciencias Veterinarias
3104.07 Ovinos
2412.10 Vacunas
description [EN]Background/Aims: Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study were to develop an experimental TB infection model in sheep with a Mycobacterium caprae field strain isolated from a tuberculous diseased ewe, and to use this to evaluate the safety and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. Methods: Eighteen 2 month-old lambs were experimentally challenged with M. caprae by the endotracheal route (1.5 × 103 CFU). They were separated per treatment group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n = 6). Clinical, immunological, pathological and bacteriological parameters of infection were measured. Results: All lambs were successfully infected and developed gross TB lesions in the respiratory system. The BCG vaccine conferred considerable protection against experimental TB in lambs, as measured by a reduction of the gross lesion volumes and bacterial load. However, HIMB vaccinated animals did not show protection. Conclusions: This study proposes a reliable new experimental model for a better understanding of tuberculosis in sheep. BCG vaccination offers an effective prospect for controlling the disease. Moreover alternative doses and/or routes of administration should be considered to evaluate the efficacy of the HIMB vaccine candidate.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180546
https://hdl.handle.net/10612/19718
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180546
https://hdl.handle.net/10612/19718
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2014-00002-C02-01
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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