Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability

Aboy-Pardal , Maria C M; Guadamillas Mora, Marta Carmen; Guerrero , Carlos R; Catala Montoro , Mauro; Toledano-Donado , Mónica; Terrés Domínguez, Sara; Pavón , Dácil M; Jiménez Jiménez, Víctor; Jiménez Carretero, Daniel; Zamai , Moreno; Folgueira , Cintia; Cerezo , Ana; Lolo , Fidel-Nicolas; Nogueiras , Rubén; Sabio , Guadalupe; Sánchez Álvarez, Miguel; Echarri , Asier; García , Ricardo; del Pozo , Miguel A

Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability

Adipocytes expand massively to accommodate excess energy stores and protect the organism from lipotoxicity. Adipose tissue expandability is at the center of disorders such as obesity and lipodystrophy; however, little is known about the relevance of adipocyte biomechanics on the etiology of these co...

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Autores:	Aboy-Pardal , Maria C M, Guadamillas Mora, Marta Carmen, Guerrero , Carlos R, Catala Montoro , Mauro, Toledano-Donado , Mónica, Terrés Domínguez, Sara, Pavón , Dácil M, Jiménez Jiménez, Víctor, Jiménez Carretero, Daniel, Zamai , Moreno, Folgueira , Cintia, Cerezo , Ana, Lolo , Fidel-Nicolas, Nogueiras , Rubén, Sabio , Guadalupe, Sánchez Álvarez, Miguel, Echarri , Asier, García , Ricardo, del Pozo , Miguel A
Tipo de recurso:	artículo
Fecha de publicación:	2024
País:	España
Institución:	Universidad de Castilla-La Mancha
Repositorio:	RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:	oai:ruidera.uclm.es:10578/46322
Acceso en línea:	https://doi.org/10.1038/s41467-024-54224-y https://hdl.handle.net/10578/46322
Access Level:	acceso abierto
Palabra clave:	Adipocytes Caveolae Lipid droplet Mechanical adaptability Obesity Phosphoregulation Plasma membrane

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spelling	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptabilityAboy-Pardal , Maria C MGuadamillas Mora, Marta CarmenGuerrero , Carlos RCatala Montoro , MauroToledano-Donado , MónicaTerrés Domínguez, SaraPavón , Dácil MJiménez Jiménez, VíctorJiménez Carretero, DanielZamai , MorenoFolgueira , CintiaCerezo , AnaLolo , Fidel-NicolasNogueiras , RubénSabio , GuadalupeSánchez Álvarez, MiguelEcharri , AsierGarcía , Ricardodel Pozo , Miguel AAdipocytesCaveolaeLipid dropletMechanical adaptabilityObesityPhosphoregulationPlasma membraneAdipocytes expand massively to accommodate excess energy stores and protect the organism from lipotoxicity. Adipose tissue expandability is at the center of disorders such as obesity and lipodystrophy; however, little is known about the relevance of adipocyte biomechanics on the etiology of these conditions. Here, we show in male mice in vivo that the adipocyte plasma membrane undergoes caveolar domain reorganization upon lipid droplet expansion. As the lipid droplet grows, caveolae disassemble to release their membrane reservoir and increase cell surface area, and transfer specific caveolar components to the LD surface. Adipose tissue null for caveolae is stiffer, shows compromised deformability, and is prone to rupture under mechanical compression. Mechanistically, phosphoacceptor Cav1 Tyr14 is required for caveolae disassembly: adipocytes bearing a Tyr14Phe mutation at this residue are stiffer and smaller, leading to decreased adiposity in vivo; exhibit deficient transfer of Cav1 and EHD2 to the LD surface, and show distinct Cav1 molecular dynamics and tension adaptation. These results indicate that Cav1 phosphoregulation modulates caveolar dynamics as a relevant component of the homeostatic mechanoadaptation of the differentiated adipocyte.NATURE PUBLISHING GROUP202620262024info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://doi.org/10.1038/s41467-024-54224-yhttps://hdl.handle.net/10578/46322reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésSAF2017-83130-RMINSEV 1512-07-2016BFU2016-81912-REDCPID2020-118658RB-I00Tec4Bio-CM, S2018/NMT¬4443201936-30-31PROYE20089DELPHR20-00075BIOPOL, 641639RYC2020-029690)PID2021−128106NA-I00MINECO-PID2019-104399RB-I00MCIN/AEI/10.13039/501100011033CEX2020-001041-SCEX2020-001041-Sinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/463222026-05-27T07:36:41Z
dc.title.none.fl_str_mv	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
title	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
spellingShingle	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability Aboy-Pardal , Maria C M Adipocytes Caveolae Lipid droplet Mechanical adaptability Obesity Phosphoregulation Plasma membrane
title_short	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
title_full	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
title_fullStr	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
title_full_unstemmed	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
title_sort	Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
dc.creator.none.fl_str_mv	Aboy-Pardal , Maria C M Guadamillas Mora, Marta Carmen Guerrero , Carlos R Catala Montoro , Mauro Toledano-Donado , Mónica Terrés Domínguez, Sara Pavón , Dácil M Jiménez Jiménez, Víctor Jiménez Carretero, Daniel Zamai , Moreno Folgueira , Cintia Cerezo , Ana Lolo , Fidel-Nicolas Nogueiras , Rubén Sabio , Guadalupe Sánchez Álvarez, Miguel Echarri , Asier García , Ricardo del Pozo , Miguel A
author	Aboy-Pardal , Maria C M
author_facet	Aboy-Pardal , Maria C M Guadamillas Mora, Marta Carmen Guerrero , Carlos R Catala Montoro , Mauro Toledano-Donado , Mónica Terrés Domínguez, Sara Pavón , Dácil M Jiménez Jiménez, Víctor Jiménez Carretero, Daniel Zamai , Moreno Folgueira , Cintia Cerezo , Ana Lolo , Fidel-Nicolas Nogueiras , Rubén Sabio , Guadalupe Sánchez Álvarez, Miguel Echarri , Asier García , Ricardo del Pozo , Miguel A
author_role	author
author2	Guadamillas Mora, Marta Carmen Guerrero , Carlos R Catala Montoro , Mauro Toledano-Donado , Mónica Terrés Domínguez, Sara Pavón , Dácil M Jiménez Jiménez, Víctor Jiménez Carretero, Daniel Zamai , Moreno Folgueira , Cintia Cerezo , Ana Lolo , Fidel-Nicolas Nogueiras , Rubén Sabio , Guadalupe Sánchez Álvarez, Miguel Echarri , Asier García , Ricardo del Pozo , Miguel A
author2_role	author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Adipocytes Caveolae Lipid droplet Mechanical adaptability Obesity Phosphoregulation Plasma membrane
topic	Adipocytes Caveolae Lipid droplet Mechanical adaptability Obesity Phosphoregulation Plasma membrane
description	Adipocytes expand massively to accommodate excess energy stores and protect the organism from lipotoxicity. Adipose tissue expandability is at the center of disorders such as obesity and lipodystrophy; however, little is known about the relevance of adipocyte biomechanics on the etiology of these conditions. Here, we show in male mice in vivo that the adipocyte plasma membrane undergoes caveolar domain reorganization upon lipid droplet expansion. As the lipid droplet grows, caveolae disassemble to release their membrane reservoir and increase cell surface area, and transfer specific caveolar components to the LD surface. Adipose tissue null for caveolae is stiffer, shows compromised deformability, and is prone to rupture under mechanical compression. Mechanistically, phosphoacceptor Cav1 Tyr14 is required for caveolae disassembly: adipocytes bearing a Tyr14Phe mutation at this residue are stiffer and smaller, leading to decreased adiposity in vivo; exhibit deficient transfer of Cav1 and EHD2 to the LD surface, and show distinct Cav1 molecular dynamics and tension adaptation. These results indicate that Cav1 phosphoregulation modulates caveolar dynamics as a relevant component of the homeostatic mechanoadaptation of the differentiated adipocyte.
publishDate	2024
dc.date.none.fl_str_mv	2024 2026 2026
dc.type.none.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://doi.org/10.1038/s41467-024-54224-y https://hdl.handle.net/10578/46322
url	https://doi.org/10.1038/s41467-024-54224-y https://hdl.handle.net/10578/46322
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	SAF2017-83130-R MINSEV 1512-07-2016 BFU2016-81912-REDC PID2020-118658RB-I00 Tec4Bio-CM, S2018/NMT¬4443 201936-30-31 PROYE20089DELP HR20-00075 BIOPOL, 641639 RYC2020-029690) PID2021−128106NA-I00 MINECO-PID2019-104399RB-I00 MCIN/AEI/10.13039/501100011033 CEX2020-001041-S CEX2020-001041-S
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	NATURE PUBLISHING GROUP
publisher.none.fl_str_mv	NATURE PUBLISHING GROUP
dc.source.none.fl_str_mv	reponame:RUIdeRA. Repositorio Institucional de la UCLM instname:Universidad de Castilla-La Mancha
instname_str	Universidad de Castilla-La Mancha
reponame_str	RUIdeRA. Repositorio Institucional de la UCLM
collection	RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.812429

Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability

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