Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation

Polar flagella from mesophilic Aeromonas strains have previously been shown to be modified with a range of glycans. Mass spectrometry studies of purified polar flagellins suggested the glycan typically includes a putative pseudaminic acid like derivative; while some strains are modified with this si...

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Autores: Forn-Cuní, Gabriel, Fulton, Kelly M., Smith, Jeffrey C., Twine, Susan M., Mendoza-Barberá, Elena, Tomàs Magaña, Juan, Merino Montero, Susana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/176524
Acceso en línea:https://hdl.handle.net/2445/176524
Access Level:acceso abierto
Palabra clave:Flagel·lats
Bacteris
Flagellata
Bacteria
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spelling Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylationForn-Cuní, GabrielFulton, Kelly M.Smith, Jeffrey C.Twine, Susan M.Mendoza-Barberá, ElenaTomàs Magaña, JuanMerino Montero, SusanaFlagel·latsBacterisFlagellataBacteriaPolar flagella from mesophilic Aeromonas strains have previously been shown to be modified with a range of glycans. Mass spectrometry studies of purified polar flagellins suggested the glycan typically includes a putative pseudaminic acid like derivative; while some strains are modified with this single monosaccharide, others modified with a heterologous glycan. In the current study, we demonstrate that genes involved in polar flagella glycosylation are clustered in highly polymorphic genomic islands flanked by pseudaminic acid biosynthetic genes (pse). Bioinformatic analysis of mesophilic Aeromonas genomes identified three types of polar flagella glycosylation islands (FGIs), denoted Group I, II and III. FGI Groups I and III are small genomic islands present in Aeromonas strains with flagellins modified with a single monosaccharide pseudaminic acid derivative. Group II were large genomic islands, present in strains found to modify polar flagellins with heterogeneous glycan moieties. Group II, in addition to pse genes, contained numerous glycosyltransferases and other biosynthetic enzymes. All Group II strains shared a common glycosyltransferase downstream of luxC that we named flagella glycosylation island 1, fgi-1, in A. piscicola AH-3. We demonstrate that Fgi-1 transfers the first sugar of the heterogeneous glycan to the pseudaminic acid derivative linked to polar flagellins and could be used as marker for polysaccharidic glycosylation of Aeromonas polar flagella.Frontiers Media2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/176524Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fmicb.2020.595697Frontiers in Microbiology, 2021, vol. 11, p. 595697https://doi.org/10.3389/fmicb.2020.595697cc-by (c) Forn-Cuní, Gabriel et al., 2021http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1765242026-05-27T06:46:51Z
dc.title.none.fl_str_mv Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation
title Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation
spellingShingle Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation
Forn-Cuní, Gabriel
Flagel·lats
Bacteris
Flagellata
Bacteria
title_short Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation
title_full Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation
title_fullStr Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation
title_full_unstemmed Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation
title_sort Polar flagella glycosylation in Aeromonas: genomic characterization and involvement of a specific glycosyltransferase (Fgi-1) in heterogeneous flagella glycosylation
dc.creator.none.fl_str_mv Forn-Cuní, Gabriel
Fulton, Kelly M.
Smith, Jeffrey C.
Twine, Susan M.
Mendoza-Barberá, Elena
Tomàs Magaña, Juan
Merino Montero, Susana
author Forn-Cuní, Gabriel
author_facet Forn-Cuní, Gabriel
Fulton, Kelly M.
Smith, Jeffrey C.
Twine, Susan M.
Mendoza-Barberá, Elena
Tomàs Magaña, Juan
Merino Montero, Susana
author_role author
author2 Fulton, Kelly M.
Smith, Jeffrey C.
Twine, Susan M.
Mendoza-Barberá, Elena
Tomàs Magaña, Juan
Merino Montero, Susana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Flagel·lats
Bacteris
Flagellata
Bacteria
topic Flagel·lats
Bacteris
Flagellata
Bacteria
description Polar flagella from mesophilic Aeromonas strains have previously been shown to be modified with a range of glycans. Mass spectrometry studies of purified polar flagellins suggested the glycan typically includes a putative pseudaminic acid like derivative; while some strains are modified with this single monosaccharide, others modified with a heterologous glycan. In the current study, we demonstrate that genes involved in polar flagella glycosylation are clustered in highly polymorphic genomic islands flanked by pseudaminic acid biosynthetic genes (pse). Bioinformatic analysis of mesophilic Aeromonas genomes identified three types of polar flagella glycosylation islands (FGIs), denoted Group I, II and III. FGI Groups I and III are small genomic islands present in Aeromonas strains with flagellins modified with a single monosaccharide pseudaminic acid derivative. Group II were large genomic islands, present in strains found to modify polar flagellins with heterogeneous glycan moieties. Group II, in addition to pse genes, contained numerous glycosyltransferases and other biosynthetic enzymes. All Group II strains shared a common glycosyltransferase downstream of luxC that we named flagella glycosylation island 1, fgi-1, in A. piscicola AH-3. We demonstrate that Fgi-1 transfers the first sugar of the heterogeneous glycan to the pseudaminic acid derivative linked to polar flagellins and could be used as marker for polysaccharidic glycosylation of Aeromonas polar flagella.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176524
url https://hdl.handle.net/2445/176524
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2020.595697
Frontiers in Microbiology, 2021, vol. 11, p. 595697
https://doi.org/10.3389/fmicb.2020.595697
dc.rights.none.fl_str_mv cc-by (c) Forn-Cuní, Gabriel et al., 2021
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Forn-Cuní, Gabriel et al., 2021
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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