In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains

In view of the current increase and spread of antimicrobial resistance (AMR), there is an urgent need to find new strategies to combat it. This study had two aims. First, we synthesized highly monodispersed silver nanoparticles (AgNPs) of approximately 17 nm, and we functionalized them with mercapto...

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Autores: Palau Gauthier, Marta, Muñoz, Estela, Freixanet Gusta, Muriel, Larrosa, María Nieves, Gomis, Xavier, Gilabert, Joan, Almirante, Benito, Puntes, Victor, Texidó Bartes, Robert, Gavaldà, Joan
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Ramon Llull (URL)
Repositorio:DAU Arxiu Digital de la Universitat Ramon Llull
OAI Identifier:oai:dau.url.edu:20.500.14342/4664
Acceso en línea:http://hdl.handle.net/20.500.14342/4664
https://doi.org/10.1128/spectrum.00280-23
Access Level:acceso abierto
Palabra clave:Antibacterial activity
MDR/XDR
Amikacin
Biofilms
Hyperthermia
Silver nanoparticles
Antibiòtics
Resistència als medicaments
577
615
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spelling In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing StrainsPalau Gauthier, MartaMuñoz, EstelaFreixanet Gusta, MurielLarrosa, María NievesGomis, XavierGilabert, JoanAlmirante, BenitoPuntes, VictorTexidó Bartes, RobertGavaldà, JoanAntibacterial activityMDR/XDRAmikacinBiofilmsHyperthermiaSilver nanoparticlesAntibiòticsResistència als medicaments577615In view of the current increase and spread of antimicrobial resistance (AMR), there is an urgent need to find new strategies to combat it. This study had two aims. First, we synthesized highly monodispersed silver nanoparticles (AgNPs) of approximately 17 nm, and we functionalized them with mercaptopoly(ethylene glycol) carboxylic acid (mPEG-COOH) and amikacin (AK). Second, we evaluated the antibacterial activity of this treatment (AgNPs_mPEG_AK) alone and in combination with hyperthermia against planktonic and biofilm-growing strains. AgNPs, AgNPs_mPEG, and AgNPs_mPEG_AK were characterized using a suite of spectroscopy and microscopy methods. Susceptibility to these treatments and AK was determined after 24 h and over time against 12 clinical multidrug-resistant (MDR)/extensively drug-resistant (XDR) isolates of Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The efficacy of the treatments alone and in combination with hyperthermia (1, 2, and 3 pulses at 41°C to 42°C for 15 min) was tested against the same planktonic strains using quantitative culture and against one P. aeruginosa strain growing on silicone disks using confocal laser scanning microscopy. The susceptibility studies showed that AgNPs_mPEG_AK was 10-fold more effective than AK alone, and bactericidal efficacy after 4, 8, 24, or 48 h was observed against 100% of the tested strains. The combination of AgNPs_mPEG_AK and hyperthermia eradicated 75% of the planktonic strains and exhibited significant reductions in biofilm formation by P. aeruginosa in comparison with the other treatments tested, except for AgNPs_mPEG_AK without hyperthermia. In conclusion, the combination of AgNPs_mPEG_AK and hyperthermia may be a promising therapy against MDR/XDR and biofilm-producing strains.info:eu-repo/semantics/publishedVersionAmerican Society for MicrobiologyUniversitat Ramon Llull. IQS202420242023info:eu-repo/semantics/articlep.16application/pdfhttp://hdl.handle.net/20.500.14342/4664https://doi.org/10.1128/spectrum.00280-23reponame:DAU Arxiu Digital de la Universitat Ramon Llullinstname:Universitat Ramon Llull (URL)InglésMicrobiology Spectrum 2023, 11 (3)info:eu-repo/grantAgreement/MINECO/ISCIII/FIS 01162info:eu-repo/grantAgreement/Fundació la Marató de TV3/472/U© L'autor/aAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dau.url.edu:20.500.14342/46642026-06-21T06:40:37Z
dc.title.none.fl_str_mv In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains
title In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains
spellingShingle In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains
Palau Gauthier, Marta
Antibacterial activity
MDR/XDR
Amikacin
Biofilms
Hyperthermia
Silver nanoparticles
Antibiòtics
Resistència als medicaments
577
615
title_short In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains
title_full In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains
title_fullStr In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains
title_full_unstemmed In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains
title_sort In Vitro Antibacterial Activity of Silver Nanoparticles Conjugated with Amikacin and Combined with Hyperthermia against Drug-Resistant and Biofilm-Producing Strains
dc.creator.none.fl_str_mv Palau Gauthier, Marta
Muñoz, Estela
Freixanet Gusta, Muriel
Larrosa, María Nieves
Gomis, Xavier
Gilabert, Joan
Almirante, Benito
Puntes, Victor
Texidó Bartes, Robert
Gavaldà, Joan
author Palau Gauthier, Marta
author_facet Palau Gauthier, Marta
Muñoz, Estela
Freixanet Gusta, Muriel
Larrosa, María Nieves
Gomis, Xavier
Gilabert, Joan
Almirante, Benito
Puntes, Victor
Texidó Bartes, Robert
Gavaldà, Joan
author_role author
author2 Muñoz, Estela
Freixanet Gusta, Muriel
Larrosa, María Nieves
Gomis, Xavier
Gilabert, Joan
Almirante, Benito
Puntes, Victor
Texidó Bartes, Robert
Gavaldà, Joan
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Antibacterial activity
MDR/XDR
Amikacin
Biofilms
Hyperthermia
Silver nanoparticles
Antibiòtics
Resistència als medicaments
577
615
topic Antibacterial activity
MDR/XDR
Amikacin
Biofilms
Hyperthermia
Silver nanoparticles
Antibiòtics
Resistència als medicaments
577
615
description In view of the current increase and spread of antimicrobial resistance (AMR), there is an urgent need to find new strategies to combat it. This study had two aims. First, we synthesized highly monodispersed silver nanoparticles (AgNPs) of approximately 17 nm, and we functionalized them with mercaptopoly(ethylene glycol) carboxylic acid (mPEG-COOH) and amikacin (AK). Second, we evaluated the antibacterial activity of this treatment (AgNPs_mPEG_AK) alone and in combination with hyperthermia against planktonic and biofilm-growing strains. AgNPs, AgNPs_mPEG, and AgNPs_mPEG_AK were characterized using a suite of spectroscopy and microscopy methods. Susceptibility to these treatments and AK was determined after 24 h and over time against 12 clinical multidrug-resistant (MDR)/extensively drug-resistant (XDR) isolates of Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The efficacy of the treatments alone and in combination with hyperthermia (1, 2, and 3 pulses at 41°C to 42°C for 15 min) was tested against the same planktonic strains using quantitative culture and against one P. aeruginosa strain growing on silicone disks using confocal laser scanning microscopy. The susceptibility studies showed that AgNPs_mPEG_AK was 10-fold more effective than AK alone, and bactericidal efficacy after 4, 8, 24, or 48 h was observed against 100% of the tested strains. The combination of AgNPs_mPEG_AK and hyperthermia eradicated 75% of the planktonic strains and exhibited significant reductions in biofilm formation by P. aeruginosa in comparison with the other treatments tested, except for AgNPs_mPEG_AK without hyperthermia. In conclusion, the combination of AgNPs_mPEG_AK and hyperthermia may be a promising therapy against MDR/XDR and biofilm-producing strains.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/4664
https://doi.org/10.1128/spectrum.00280-23
url http://hdl.handle.net/20.500.14342/4664
https://doi.org/10.1128/spectrum.00280-23
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microbiology Spectrum 2023, 11 (3)
info:eu-repo/grantAgreement/MINECO/ISCIII/FIS 01162
info:eu-repo/grantAgreement/Fundació la Marató de TV3/472/U
dc.rights.none.fl_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv p.16
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DAU Arxiu Digital de la Universitat Ramon Llull
instname:Universitat Ramon Llull (URL)
instname_str Universitat Ramon Llull (URL)
reponame_str DAU Arxiu Digital de la Universitat Ramon Llull
collection DAU Arxiu Digital de la Universitat Ramon Llull
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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