Fluorescence-based biosensors for west nile virus detection

Flaviviruses are emerging pathogens, mostly transmitted by arthropod vectors, responsible for human, animal, and zoonotic diseases. The emergence of flaviviruses has been favored in recent decades by factors related to climate change and globalization, which contribute to the arrival and establishme...

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Authors: Blázquez, Ana Belén, Saiz, Juan Carlos, Herrero, Laura, Sanchez Cespedes, Javier, Vázquez, Ana, Cisneros, José Miguel, Martin-Acebes, Miguel A., Jiménez de Oya, Nereida
Format: article
Status:Published version
Publication Date:2025
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/180109
Online Access:https://hdl.handle.net/11441/180109
https://doi.org/10.1080/21505594.2025.2597663
Access Level:Open access
Keyword:Arboviruses
West Nile virus
Antivirals
Biosensors
Diagnosis
Flaviviruses
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spelling Fluorescence-based biosensors for west nile virus detectionBlázquez, Ana BelénSaiz, Juan CarlosHerrero, LauraSanchez Cespedes, JavierVázquez, AnaCisneros, José MiguelMartin-Acebes, Miguel A.Jiménez de Oya, NereidaArbovirusesWest Nile virusAntiviralsBiosensorsDiagnosisFlavivirusesFlaviviruses are emerging pathogens, mostly transmitted by arthropod vectors, responsible for human, animal, and zoonotic diseases. The emergence of flaviviruses has been favored in recent decades by factors related to climate change and globalization, which contribute to the arrival and establishment of their vectors in new geographic areas, thus promoting epidemic outbreaks and facilitating these viruses to become endemic in these areas. This is the case of the West Nile virus (WNV) in Europe, which affects its natural bird host populations and also accidental hosts such as humans and horses. Flaviviruses are antigenically related, which induces cross-reactivity, making their serological diagnosis difficult, especially in areas where several flaviviruses co-circulate. Here, we have developed WNV biosensors in which the enzymatic activity of the viral protease, expressed during infection, allows its detection using fluorescence-based techniques. These biosensors carry WNV-specific protease cleavage sites and show high specificity for the detection of both lineages 1 and 2, with limits of detection (LODs) ranging from 0.001-0.0001 MOI at 48 hours post-infection (h.p.i.). These LODs are even lower at 72 h.p.i. reaching as low as an MOI of 2.5 × 10-6. They are also capable of detecting, although with lower sensitivity, other flaviviruses such as dengue, Zika, and Usutu viruses, without showing reactivity against unrelated viruses. These biosensors have been validated in viral neutralization assays with sera from infected mice and humans, as well as in antiviral screening, with results comparable to those of currently used systems, showing significant potential as clinical and laboratory tools.Taylor & francis inc; Informa UK LimitedMedicinaInstituto de Biomedicina de Sevilla (IBIS)2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/180109https://doi.org/10.1080/21505594.2025.2597663reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésVirulence, 16 (1), 2597663.https://www.tandfonline.com/doi/full/10.1080/21505594.2025.2597663#abstractinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/1801092026-06-17T12:51:07Z
dc.title.none.fl_str_mv Fluorescence-based biosensors for west nile virus detection
title Fluorescence-based biosensors for west nile virus detection
spellingShingle Fluorescence-based biosensors for west nile virus detection
Blázquez, Ana Belén
Arboviruses
West Nile virus
Antivirals
Biosensors
Diagnosis
Flaviviruses
title_short Fluorescence-based biosensors for west nile virus detection
title_full Fluorescence-based biosensors for west nile virus detection
title_fullStr Fluorescence-based biosensors for west nile virus detection
title_full_unstemmed Fluorescence-based biosensors for west nile virus detection
title_sort Fluorescence-based biosensors for west nile virus detection
dc.creator.none.fl_str_mv Blázquez, Ana Belén
Saiz, Juan Carlos
Herrero, Laura
Sanchez Cespedes, Javier
Vázquez, Ana
Cisneros, José Miguel
Martin-Acebes, Miguel A.
Jiménez de Oya, Nereida
author Blázquez, Ana Belén
author_facet Blázquez, Ana Belén
Saiz, Juan Carlos
Herrero, Laura
Sanchez Cespedes, Javier
Vázquez, Ana
Cisneros, José Miguel
Martin-Acebes, Miguel A.
Jiménez de Oya, Nereida
author_role author
author2 Saiz, Juan Carlos
Herrero, Laura
Sanchez Cespedes, Javier
Vázquez, Ana
Cisneros, José Miguel
Martin-Acebes, Miguel A.
Jiménez de Oya, Nereida
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Medicina
Instituto de Biomedicina de Sevilla (IBIS)
dc.subject.none.fl_str_mv Arboviruses
West Nile virus
Antivirals
Biosensors
Diagnosis
Flaviviruses
topic Arboviruses
West Nile virus
Antivirals
Biosensors
Diagnosis
Flaviviruses
description Flaviviruses are emerging pathogens, mostly transmitted by arthropod vectors, responsible for human, animal, and zoonotic diseases. The emergence of flaviviruses has been favored in recent decades by factors related to climate change and globalization, which contribute to the arrival and establishment of their vectors in new geographic areas, thus promoting epidemic outbreaks and facilitating these viruses to become endemic in these areas. This is the case of the West Nile virus (WNV) in Europe, which affects its natural bird host populations and also accidental hosts such as humans and horses. Flaviviruses are antigenically related, which induces cross-reactivity, making their serological diagnosis difficult, especially in areas where several flaviviruses co-circulate. Here, we have developed WNV biosensors in which the enzymatic activity of the viral protease, expressed during infection, allows its detection using fluorescence-based techniques. These biosensors carry WNV-specific protease cleavage sites and show high specificity for the detection of both lineages 1 and 2, with limits of detection (LODs) ranging from 0.001-0.0001 MOI at 48 hours post-infection (h.p.i.). These LODs are even lower at 72 h.p.i. reaching as low as an MOI of 2.5 × 10-6. They are also capable of detecting, although with lower sensitivity, other flaviviruses such as dengue, Zika, and Usutu viruses, without showing reactivity against unrelated viruses. These biosensors have been validated in viral neutralization assays with sera from infected mice and humans, as well as in antiviral screening, with results comparable to those of currently used systems, showing significant potential as clinical and laboratory tools.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/180109
https://doi.org/10.1080/21505594.2025.2597663
url https://hdl.handle.net/11441/180109
https://doi.org/10.1080/21505594.2025.2597663
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Virulence, 16 (1), 2597663.
https://www.tandfonline.com/doi/full/10.1080/21505594.2025.2597663#abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & francis inc; Informa UK Limited
publisher.none.fl_str_mv Taylor & francis inc; Informa UK Limited
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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