Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin

Methicillin-resistant Staphylococcus aureus (MRSA) is a prevailing bacterial pathogen linked to superficial skin and soft tissue infections (SSTIs). Rifampicin (RIF), a potent antibiotic against systemic and localised staphylococcal infections, faces limitations due to its low solubility. This const...

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Autores: Anjani, Qonita Kurnia, Pandya, Anjali K., Demartis, Sara, Domínguez Robles, Juan, Moreno Castellanos, Natalia, Li, Huanhuan, Gavini, Elisabetta, Patravale, Vandana B., Donnelly, Ryan F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/155764
Acceso en línea:https://hdl.handle.net/11441/155764
https://doi.org/10.1016/j.ijpharm.2023.123446
Access Level:acceso abierto
Palabra clave:Dissolving microneedles
Liposome
MRSA
Rifampicin
Skin infection
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spelling Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of RifampicinAnjani, Qonita KurniaPandya, Anjali K.Demartis, SaraDomínguez Robles, JuanMoreno Castellanos, NataliaLi, HuanhuanGavini, ElisabettaPatravale, Vandana B.Donnelly, Ryan F.Dissolving microneedlesLiposomeMRSARifampicinSkin infectionMethicillin-resistant Staphylococcus aureus (MRSA) is a prevailing bacterial pathogen linked to superficial skin and soft tissue infections (SSTIs). Rifampicin (RIF), a potent antibiotic against systemic and localised staphylococcal infections, faces limitations due to its low solubility. This constraint hampers its therapeutic potential for MRSA-induced SSTIs. To address this, an advanced liposomal system was designed for efficient dermal RIF delivery. Rifampicin-loaded liposomes (LipoRIF) were embedded within polymeric dissolving microneedles (DMNs) to enable targeted intradermal drug delivery. A robust Design of Experiment (DoE) methodology guided the systematic preparation and optimisation of LipoRIF formulations. The optimal LipoRIF formulation integrated within polymeric DMNs. These LipoRIF-DMNs exhibited favourable mechanical properties and effective skin insertion characteristics. Notably, in vitro assays on skin deposition unveiled a transformative result – the DMN platform significantly enhanced LipoRIF deposition within the skin, surpassing LipoRIF dispersion alone. Moreover, LipoRIF-DMNs displayed minimal cytotoxicity toward cells. Encouragingly, rigorous in vitro antimicrobial evaluations demonstrated LipoRIF-DMNs' capacity to inhibit MRSA growth compared to the control group. LipoRIF-DMNs propose a potentially enhanced, minimally invasive approach to effectively manage SSTIs and superficial skin ailments stemming from MRSA infections.Wellcome Trust WT094085MAElsevierFarmacia y Tecnología FarmacéuticaWellcome Trust2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/155764https://doi.org/10.1016/j.ijpharm.2023.123446reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Pharmaceutics, 646, 123446.WT094085MAhttps://doi.org/10.1016/j.ijpharm.2023.123446info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1557642026-06-17T12:51:07Z
dc.title.none.fl_str_mv Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
title Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
spellingShingle Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
Anjani, Qonita Kurnia
Dissolving microneedles
Liposome
MRSA
Rifampicin
Skin infection
title_short Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
title_full Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
title_fullStr Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
title_full_unstemmed Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
title_sort Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
dc.creator.none.fl_str_mv Anjani, Qonita Kurnia
Pandya, Anjali K.
Demartis, Sara
Domínguez Robles, Juan
Moreno Castellanos, Natalia
Li, Huanhuan
Gavini, Elisabetta
Patravale, Vandana B.
Donnelly, Ryan F.
author Anjani, Qonita Kurnia
author_facet Anjani, Qonita Kurnia
Pandya, Anjali K.
Demartis, Sara
Domínguez Robles, Juan
Moreno Castellanos, Natalia
Li, Huanhuan
Gavini, Elisabetta
Patravale, Vandana B.
Donnelly, Ryan F.
author_role author
author2 Pandya, Anjali K.
Demartis, Sara
Domínguez Robles, Juan
Moreno Castellanos, Natalia
Li, Huanhuan
Gavini, Elisabetta
Patravale, Vandana B.
Donnelly, Ryan F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Farmacia y Tecnología Farmacéutica
Wellcome Trust
dc.subject.none.fl_str_mv Dissolving microneedles
Liposome
MRSA
Rifampicin
Skin infection
topic Dissolving microneedles
Liposome
MRSA
Rifampicin
Skin infection
description Methicillin-resistant Staphylococcus aureus (MRSA) is a prevailing bacterial pathogen linked to superficial skin and soft tissue infections (SSTIs). Rifampicin (RIF), a potent antibiotic against systemic and localised staphylococcal infections, faces limitations due to its low solubility. This constraint hampers its therapeutic potential for MRSA-induced SSTIs. To address this, an advanced liposomal system was designed for efficient dermal RIF delivery. Rifampicin-loaded liposomes (LipoRIF) were embedded within polymeric dissolving microneedles (DMNs) to enable targeted intradermal drug delivery. A robust Design of Experiment (DoE) methodology guided the systematic preparation and optimisation of LipoRIF formulations. The optimal LipoRIF formulation integrated within polymeric DMNs. These LipoRIF-DMNs exhibited favourable mechanical properties and effective skin insertion characteristics. Notably, in vitro assays on skin deposition unveiled a transformative result – the DMN platform significantly enhanced LipoRIF deposition within the skin, surpassing LipoRIF dispersion alone. Moreover, LipoRIF-DMNs displayed minimal cytotoxicity toward cells. Encouragingly, rigorous in vitro antimicrobial evaluations demonstrated LipoRIF-DMNs' capacity to inhibit MRSA growth compared to the control group. LipoRIF-DMNs propose a potentially enhanced, minimally invasive approach to effectively manage SSTIs and superficial skin ailments stemming from MRSA infections.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/155764
https://doi.org/10.1016/j.ijpharm.2023.123446
url https://hdl.handle.net/11441/155764
https://doi.org/10.1016/j.ijpharm.2023.123446
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Pharmaceutics, 646, 123446.
WT094085MA
https://doi.org/10.1016/j.ijpharm.2023.123446
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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