Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods

In this study, we validated various methods of pre-treatment of poly(ethylene terephthalate) (PET) by the engineered yeast Yarrowia lipolytica. This research compares both the effect of the type of plastic used, the processing method and enzymes with different mechanisms of action (PETase and cutina...

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Autores: Kosiorowska, Katarzyna E., Moreno, Antonio D., Iglesias, Raquel, Biniarz, Piotr, Mirończuk, Aleksandra M.
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)
Repositorio:Docu-menta. Repositorio Institucional del CIEMAT
Idioma:inglés
OAI Identifier:oai:dnet:documenta___::c569f2890dd9437fa8480479e825d64b
Acceso en línea:https://hdl.handle.net/20.500.14855/4801
Access Level:acceso abierto
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spelling Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methodsKosiorowska, Katarzyna E.Moreno, Antonio D.Iglesias, RaquelBiniarz, PiotrMirończuk, Aleksandra M.In this study, we validated various methods of pre-treatment of poly(ethylene terephthalate) (PET) by the engineered yeast Yarrowia lipolytica. This research compares both the effect of the type of plastic used, the processing method and enzymes with different mechanisms of action (PETase and cutinase). The investigation demonstrated that the degradation efficiency varies depending on the type of plastic used, the processing methods and the applied enzyme. Moreover, it indicated that during prolonged yeast culture under the applied conditions, enzyme activity is not impaired. Among all the methods tested, the artificial aging process had the greatest impact on the degradation level by PETase, where the amount of TPA released from commercial PET film was the highest, and yielded over 2 gL-1. The maximum yield of TPA (0.59 gL-1), for the Y. lipolytica strain overexpressing cutinase, was observed during the process with recycled PET bottles shredded into 1 mm fragments. The maximum recorded weight loss of plastic film is over 70% for commercial PET film subjected to artificial ageing process.Elsevier20252025-02-1520242024-08-0120242024-08-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://hdl.handle.net/20.500.14855/4801reponame:Docu-menta. Repositorio Institucional del CIEMATinstname:Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dnet:documenta___::c569f2890dd9437fa8480479e825d64b2026-06-18T12:40:47Z
dc.title.none.fl_str_mv Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
title Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
spellingShingle Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
Kosiorowska, Katarzyna E.
title_short Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
title_full Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
title_fullStr Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
title_full_unstemmed Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
title_sort Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
dc.creator.none.fl_str_mv Kosiorowska, Katarzyna E.
Moreno, Antonio D.
Iglesias, Raquel
Biniarz, Piotr
Mirończuk, Aleksandra M.
author Kosiorowska, Katarzyna E.
author_facet Kosiorowska, Katarzyna E.
Moreno, Antonio D.
Iglesias, Raquel
Biniarz, Piotr
Mirończuk, Aleksandra M.
author_role author
author2 Moreno, Antonio D.
Iglesias, Raquel
Biniarz, Piotr
Mirończuk, Aleksandra M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
description In this study, we validated various methods of pre-treatment of poly(ethylene terephthalate) (PET) by the engineered yeast Yarrowia lipolytica. This research compares both the effect of the type of plastic used, the processing method and enzymes with different mechanisms of action (PETase and cutinase). The investigation demonstrated that the degradation efficiency varies depending on the type of plastic used, the processing methods and the applied enzyme. Moreover, it indicated that during prolonged yeast culture under the applied conditions, enzyme activity is not impaired. Among all the methods tested, the artificial aging process had the greatest impact on the degradation level by PETase, where the amount of TPA released from commercial PET film was the highest, and yielded over 2 gL-1. The maximum yield of TPA (0.59 gL-1), for the Y. lipolytica strain overexpressing cutinase, was observed during the process with recycled PET bottles shredded into 1 mm fragments. The maximum recorded weight loss of plastic film is over 70% for commercial PET film subjected to artificial ageing process.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-08-01
2024
2024-08-01
2025
2025-02-15
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14855/4801
url https://hdl.handle.net/20.500.14855/4801
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docu-menta. Repositorio Institucional del CIEMAT
instname:Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)
instname_str Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)
reponame_str Docu-menta. Repositorio Institucional del CIEMAT
collection Docu-menta. Repositorio Institucional del CIEMAT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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