Functional properties of the human ventral mesencephalic neural stem cell line hVM1

The human fetal ventral mesencephalon-derived stem cell line, hVM1, yields high number of tyrosine hydroxylase-expressing presumed dopaminergic neurons upon in vitro differentiation. Here we report that cells generated from this line differentiate into a neuronal phenotype, express electrophysiologi...

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Detalles Bibliográficos
Autores: Tonnesen, Jan, González Seiz, Emma, Ramos, Milagros, Lindvall, Olle, Martínez-Serrano, Alberto, Kokaia, Merab
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/377882
Acceso en línea:http://hdl.handle.net/10261/377882
Access Level:acceso abierto
Palabra clave:Human neural stem cell
Dopaminergic neuron
In vitro
Electrophysiology
Functional properties
Differentiation
Parkinson's disease
Descripción
Sumario:The human fetal ventral mesencephalon-derived stem cell line, hVM1, yields high number of tyrosine hydroxylase-expressing presumed dopaminergic neurons upon in vitro differentiation. Here we report that cells generated from this line differentiate into a neuronal phenotype, express electrophysiological properties of functional neurons and respond to neurotransmitters in vitro. However, the electrophysiological properties are immature and the cells require longer maturation time than possible under in vitro conditions.