The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress

14 Pàg.

Detalhes bibliográficos
Autores: Hafsi, Chokri, Collado-Arenal, Aurelio M., Wang, Haiyang, Sanz-Fernández, María Victoria, Sahrawy, Mariam, Shabala, Sergey, Romero-Puertas, María C., Sandalio, Luisa M.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/274488
Acesso em linha:http://hdl.handle.net/10261/274488
https://api.elsevier.com/content/abstract/scopus_id/85123178683
Access Level:acceso abierto
Palavra-chave:Antioxidants
Arabidopsis
Cd(2+) flux
K(+) flux
NADPH oxidases
Photosynthesis
Transporters flux
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spelling The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stressHafsi, ChokriCollado-Arenal, Aurelio M.Wang, HaiyangSanz-Fernández, María VictoriaSahrawy, MariamShabala, SergeyRomero-Puertas, María C.Sandalio, Luisa M.AntioxidantsArabidopsisCd(2+) fluxK(+) fluxNADPH oxidasesPhotosynthesisTransporters fluxNADPH oxidases14 Pàg.NADPH oxidase, an enzyme associated with the plasma membrane, constitutes one of the main sources of reactive oxygen species (ROS) which regulate different developmental and adaptive responses in plants. In this work, the involvement of NADPH oxidases in the regulation of photosynthesis and cell ionic homeostasis in response to short cadmium exposure was compared between wild type (WT) and three RBOHs (Respiratory Burst Oxidase Homologues) Arabidopsis mutants (AtrbohC, AtrbohD, and AtrbohF). Plants were grown under hydroponic conditions and supplemented with 50 µM CdCl2 for 24 h. Cadmium treatment differentially affected photosynthesis, stomatal conductance, transpiration, and antioxidative responses in WT and Atrbohs mutants. The loss of function of RBOH isoforms resulted in higher Cd2+ influx, mainly in the elongation zone of roots, which was more evident in AtrbohD and AtrbohF mutants. In the mature zone, the highest Cd2+ influx was observed in rbohC mutant. The lack of functional RBOH isoforms also resulted in altered patterns of net K+ transport across cellular membranes, both in the root epidermis and leaf mesophyll. The analysis of expression of metal transporters by qPCR demonstrated that a loss of functional RBOH isoforms has altered transcript levels for metal NRAMP3, NRAMP6 and IRT1 and the K+ transporters outward-rectifying K+ efflux GORK channel, while RBOHD specifically regulated transcripts for high-affinity K+ transporters KUP8 and HAK5, and IRT1 and RBOHD and F regulated the transcription factors TGA3 and TGA10. It is concluded that RBOH-dependent H2O2 regulation of ion homeostasis and Cd is a highly complex process involving multilevel regulation from transpirational water flow to transcriptional and posttranslational modifications of K/metals transporters.C. Hafsi acknowledges Erasmus Mundus – Al Idrisi II fellowship financed by the Erasmus Mundus Programme of the European Union. This work was supported by FEDER Funds (A1123060E00013), Junta de Andalucía I+D+I grants Ref PY20_00364, and Spanish Ministry of Science and Innovation Ref PGC2018-098372-B-I00. Sergey Shabala acknowledges funding from National Natural Science Foundation of China (project 31870249), the National Distinguished Expert Project (WQ20174400441), and Joint Research Projects between Pakistan Science Foundation and National Natural Science Foundation of China (grant 31961143001).Peer reviewedElsevierEuropean CommissionJunta de AndalucíaMinisterio de Ciencia e Innovación (España)National Natural Science Foundation of ChinaPakistan Science FoundationHafsi, Chokri [0000-0002-5639-7401]Collado-Arenal, Aurelio M [0000-0002-0170-6828]Sanz-Fernandez, M. V. [0000-0001-6863-6388]Shabala, Sergey [0000-0003-2345-8981]Romero-Puertas, María C [0000-0002-4854-896X]Sandalio, Luisa M.[0000-0002-8550-7577]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/274488https://api.elsevier.com/content/abstract/scopus_id/85123178683reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/A1123060E00013/info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-098372-B-I00https://doi.org/10.1016/j.jhazmat.2022.128217Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2744882026-05-22T06:33:51Z
dc.title.none.fl_str_mv The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress
title The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress
spellingShingle The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress
Hafsi, Chokri
Antioxidants
Arabidopsis
Cd(2+) flux
K(+) flux
NADPH oxidases
Photosynthesis
Transporters flux
NADPH oxidases
title_short The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress
title_full The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress
title_fullStr The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress
title_full_unstemmed The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress
title_sort The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress
dc.creator.none.fl_str_mv Hafsi, Chokri
Collado-Arenal, Aurelio M.
Wang, Haiyang
Sanz-Fernández, María Victoria
Sahrawy, Mariam
Shabala, Sergey
Romero-Puertas, María C.
Sandalio, Luisa M.
author Hafsi, Chokri
author_facet Hafsi, Chokri
Collado-Arenal, Aurelio M.
Wang, Haiyang
Sanz-Fernández, María Victoria
Sahrawy, Mariam
Shabala, Sergey
Romero-Puertas, María C.
Sandalio, Luisa M.
author_role author
author2 Collado-Arenal, Aurelio M.
Wang, Haiyang
Sanz-Fernández, María Victoria
Sahrawy, Mariam
Shabala, Sergey
Romero-Puertas, María C.
Sandalio, Luisa M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Junta de Andalucía
Ministerio de Ciencia e Innovación (España)
National Natural Science Foundation of China
Pakistan Science Foundation
Hafsi, Chokri [0000-0002-5639-7401]
Collado-Arenal, Aurelio M [0000-0002-0170-6828]
Sanz-Fernandez, M. V. [0000-0001-6863-6388]
Shabala, Sergey [0000-0003-2345-8981]
Romero-Puertas, María C [0000-0002-4854-896X]
Sandalio, Luisa M.[0000-0002-8550-7577]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antioxidants
Arabidopsis
Cd(2+) flux
K(+) flux
NADPH oxidases
Photosynthesis
Transporters flux
NADPH oxidases
topic Antioxidants
Arabidopsis
Cd(2+) flux
K(+) flux
NADPH oxidases
Photosynthesis
Transporters flux
NADPH oxidases
description 14 Pàg.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/274488
https://api.elsevier.com/content/abstract/scopus_id/85123178683
url http://hdl.handle.net/10261/274488
https://api.elsevier.com/content/abstract/scopus_id/85123178683
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/A1123060E00013/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-098372-B-I00
https://doi.org/10.1016/j.jhazmat.2022.128217
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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