Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry

Producción Científica

Detalles Bibliográficos
Autores: Ares Sacristán, Ana María, Toribio Recio, Laura, Nozal Nalda, María Jesús del, Martín Gómez, María Teresa, Bernal del Nozal, José
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/65300
Acceso en línea:https://doi.org/10.1016/j.microc.2020.105000
https://uvadoc.uva.es/handle/10324/65300
Access Level:acceso abierto
Palabra clave:Bee pollen
Betaines
Core-shell columns
Hydrophilic interaction liquid chromatography
Mass spectrometry
Sample treatment
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spelling Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometryAres Sacristán, Ana MaríaToribio Recio, LauraNozal Nalda, María Jesús delMartín Gómez, María TeresaBernal del Nozal, JoséBee pollenBetainesCore-shell columnsHydrophilic interaction liquid chromatographyMass spectrometrySample treatmentProducción CientíficaThe objective of this work was to develop a new method to determine nine betaines and other quaternary ammonium related compounds in bee pollen from different botanical origins; hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) was the technique employed. A quick and efficient sample treatment (average analyte recoveries were between 82% and 95% and no significant matrix effect on ionization was found), involving an extraction with an acetonitrile and water mixture (1:1, v/v), centrifugation, freezing with dry ice followed by dilution, was presented. Chromatographic analysis (15 min) was by means of a core–shell HILIC column and a mobile phase applied in gradient elution mode. The analytical characteristics of the method were evaluated and the data demonstrated that not only was it selective, but it also displayed a wide linearity range and good precision. Several bee pollen samples from different botanical origins were analyzed with the proposed methodology, and three betaines (betaine, choline and trigonelline) were detected in all of them in a wide range of concentrations (57–62236 mg/kg).Este trabajo forma parte de los proyectos de investigación financiados por el Ministerio de Economía y Competitividad e INIA-FEDER (RTA2015-00013-C03-01 y 03).Elsevier2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/j.microc.2020.105000https://uvadoc.uva.es/handle/10324/65300reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/pii/S0026265X20310250?via%3Dihubinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/653002026-06-13T12:44:47Z
dc.title.none.fl_str_mv Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
title Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
spellingShingle Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
Ares Sacristán, Ana María
Bee pollen
Betaines
Core-shell columns
Hydrophilic interaction liquid chromatography
Mass spectrometry
Sample treatment
title_short Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
title_full Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
title_fullStr Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
title_full_unstemmed Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
title_sort Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
dc.creator.none.fl_str_mv Ares Sacristán, Ana María
Toribio Recio, Laura
Nozal Nalda, María Jesús del
Martín Gómez, María Teresa
Bernal del Nozal, José
author Ares Sacristán, Ana María
author_facet Ares Sacristán, Ana María
Toribio Recio, Laura
Nozal Nalda, María Jesús del
Martín Gómez, María Teresa
Bernal del Nozal, José
author_role author
author2 Toribio Recio, Laura
Nozal Nalda, María Jesús del
Martín Gómez, María Teresa
Bernal del Nozal, José
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bee pollen
Betaines
Core-shell columns
Hydrophilic interaction liquid chromatography
Mass spectrometry
Sample treatment
topic Bee pollen
Betaines
Core-shell columns
Hydrophilic interaction liquid chromatography
Mass spectrometry
Sample treatment
description Producción Científica
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.microc.2020.105000
https://uvadoc.uva.es/handle/10324/65300
url https://doi.org/10.1016/j.microc.2020.105000
https://uvadoc.uva.es/handle/10324/65300
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0026265X20310250?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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