Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients

Background: Bipolar disorder (BD) is a severe, chronic mental illness that remains difficult to diagnose due to the lack of specific biomarkers, relying primarily on clinical assessments. Early diagnosis and treatment are essential for improving prognosis and lowering suicide risk. This study aimed...

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Autores: Delgado-Sequera, Alejandra, Pérez-Revuelta, José I., Caballero-García, Andrés, Durán-Ruiz, Mª Carmen, Romero-López-Alberca, Cristina, García-Mompó, Clara, González-Saiz, Francisco, Rodríguez-Iglesias, Manuel, Sanchez-Morillo, Daniel, Robledo, Patricia, 1958-, Pérez Solà, Víctor, Berrocoso, Esther, Hidalgo-Figueroa, María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/72198
Acceso en línea:https://hdl.handle.net/10230/72198
http://dx.doi.org/10.1186/s10020-024-01039-8
Access Level:acceso abierto
Palabra clave:Biomarker
Bipolar disorder
Cytoskeleton
Olfactory neuroepithelium
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spelling Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patientsDelgado-Sequera, AlejandraPérez-Revuelta, José I.Caballero-García, AndrésDurán-Ruiz, Mª CarmenRomero-López-Alberca, CristinaGarcía-Mompó, ClaraGonzález-Saiz, FranciscoRodríguez-Iglesias, ManuelSanchez-Morillo, DanielRobledo, Patricia, 1958-Pérez Solà, VíctorBerrocoso, EstherHidalgo-Figueroa, MaríaBiomarkerBipolar disorderCytoskeletonOlfactory neuroepitheliumBackground: Bipolar disorder (BD) is a severe, chronic mental illness that remains difficult to diagnose due to the lack of specific biomarkers, relying primarily on clinical assessments. Early diagnosis and treatment are essential for improving prognosis and lowering suicide risk. This study aimed to identify biomarkers and therapeutic targets by utilizing olfactory neuroepithelium (ONE) cells from patients with BD and controls. Methods: Immunofluorescence of ONE cells, along with proteomic and RNA sequencing analyses, was performed to investigate cytoskeletal changes and pathways involved in cell adhesion, movement, and morphology. Additionally, potential biomarkers were investigated in blood samples to improve clinical accessibility. Results: Thus, according to functional assays, ONE cells derived from BD patients exhibited decreased substrate adhesion, reduced cell migration, and morphological changes compared to control cells. In addition, proteomic and RNAseq analyses in ONE cells and peripheral blood mononuclear cells (PBMCs) revealed alterations in pathways such as RhoA/PAK/Integrin and Actin Cytoskeleton Signaling, as well as significant changes in inflammatory and immunological pathways. AUROC analysis identified proteins like PTK2 as potential diagnostic biomarkers, showing altered expression in both ONE cells and PBMCs. PTK2 RNA expression correlated with distinct morphological traits in BD ONE cells. Conclusions: In summary, this study identified cytoskeletal alterations, reduced adhesion, and disrupted migration patterns in BD ONE cells, highlighting molecular mechanisms underlying these changes and emphasizing PTK2's role as a potential diagnostic biomarker for BD.BioMed Central2026202620242026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/10230/72198http://dx.doi.org/10.1186/s10020-024-01039-8reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésMolecular Medicine. 2024;30(1):271© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/721982026-05-29T05:05:01Z
dc.title.none.fl_str_mv Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients
title Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients
spellingShingle Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients
Delgado-Sequera, Alejandra
Biomarker
Bipolar disorder
Cytoskeleton
Olfactory neuroepithelium
title_short Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients
title_full Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients
title_fullStr Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients
title_full_unstemmed Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients
title_sort Distinct patterns of cell adhesion, migration, and morphology in olfactory neuroepithelium cells of bipolar disorder patients
dc.creator.none.fl_str_mv Delgado-Sequera, Alejandra
Pérez-Revuelta, José I.
Caballero-García, Andrés
Durán-Ruiz, Mª Carmen
Romero-López-Alberca, Cristina
García-Mompó, Clara
González-Saiz, Francisco
Rodríguez-Iglesias, Manuel
Sanchez-Morillo, Daniel
Robledo, Patricia, 1958-
Pérez Solà, Víctor
Berrocoso, Esther
Hidalgo-Figueroa, María
author Delgado-Sequera, Alejandra
author_facet Delgado-Sequera, Alejandra
Pérez-Revuelta, José I.
Caballero-García, Andrés
Durán-Ruiz, Mª Carmen
Romero-López-Alberca, Cristina
García-Mompó, Clara
González-Saiz, Francisco
Rodríguez-Iglesias, Manuel
Sanchez-Morillo, Daniel
Robledo, Patricia, 1958-
Pérez Solà, Víctor
Berrocoso, Esther
Hidalgo-Figueroa, María
author_role author
author2 Pérez-Revuelta, José I.
Caballero-García, Andrés
Durán-Ruiz, Mª Carmen
Romero-López-Alberca, Cristina
García-Mompó, Clara
González-Saiz, Francisco
Rodríguez-Iglesias, Manuel
Sanchez-Morillo, Daniel
Robledo, Patricia, 1958-
Pérez Solà, Víctor
Berrocoso, Esther
Hidalgo-Figueroa, María
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biomarker
Bipolar disorder
Cytoskeleton
Olfactory neuroepithelium
topic Biomarker
Bipolar disorder
Cytoskeleton
Olfactory neuroepithelium
description Background: Bipolar disorder (BD) is a severe, chronic mental illness that remains difficult to diagnose due to the lack of specific biomarkers, relying primarily on clinical assessments. Early diagnosis and treatment are essential for improving prognosis and lowering suicide risk. This study aimed to identify biomarkers and therapeutic targets by utilizing olfactory neuroepithelium (ONE) cells from patients with BD and controls. Methods: Immunofluorescence of ONE cells, along with proteomic and RNA sequencing analyses, was performed to investigate cytoskeletal changes and pathways involved in cell adhesion, movement, and morphology. Additionally, potential biomarkers were investigated in blood samples to improve clinical accessibility. Results: Thus, according to functional assays, ONE cells derived from BD patients exhibited decreased substrate adhesion, reduced cell migration, and morphological changes compared to control cells. In addition, proteomic and RNAseq analyses in ONE cells and peripheral blood mononuclear cells (PBMCs) revealed alterations in pathways such as RhoA/PAK/Integrin and Actin Cytoskeleton Signaling, as well as significant changes in inflammatory and immunological pathways. AUROC analysis identified proteins like PTK2 as potential diagnostic biomarkers, showing altered expression in both ONE cells and PBMCs. PTK2 RNA expression correlated with distinct morphological traits in BD ONE cells. Conclusions: In summary, this study identified cytoskeletal alterations, reduced adhesion, and disrupted migration patterns in BD ONE cells, highlighting molecular mechanisms underlying these changes and emphasizing PTK2's role as a potential diagnostic biomarker for BD.
publishDate 2024
dc.date.none.fl_str_mv 2024
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10230/72198
http://dx.doi.org/10.1186/s10020-024-01039-8
url https://hdl.handle.net/10230/72198
http://dx.doi.org/10.1186/s10020-024-01039-8
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Medicine. 2024;30(1):271
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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