Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics

Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the c...

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Detalhes bibliográficos
Autores: Rojas-Gómez, Amelia, Dosil, S. G., Chichón, Francisco Javier, Fernández-Gallego, Nieves, Ferrarini, Alessia, Calvo, Enrique, Calzada-Fraile, Diego, Requena, Silvia, Otón, Joaquín, Serrano, Álvaro, Tarifa, Rocío, Arroyo, Montserrat, Sorrentino, A., Pereiro, Eva, Vázquez, Jesús, Valpuesta, José M., Sánchez-Madrid, Francisco, Martín-Cófreces, Noa Beatriz
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/362902
Acesso em linha:http://hdl.handle.net/10261/362902
https://api.elsevier.com/content/abstract/scopus_id/85162082519
Access Level:Acceso aberto
Palavra-chave:CCT
chaperonin
extracellular vesicle
lipid droplet
lipidomic
peroxisome
id ES_e974aaea68029db97847b0f854ae3a99
oai_identifier_str oai:digital.csic.es:10261/362902
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics
title Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics
spellingShingle Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics
Rojas-Gómez, Amelia
CCT
chaperonin
extracellular vesicle
lipid droplet
lipidomic
peroxisome
title_short Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics
title_full Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics
title_fullStr Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics
title_full_unstemmed Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics
title_sort Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics
dc.creator.none.fl_str_mv Rojas-Gómez, Amelia
Dosil, S. G.
Chichón, Francisco Javier
Fernández-Gallego, Nieves
Ferrarini, Alessia
Calvo, Enrique
Calzada-Fraile, Diego
Requena, Silvia
Otón, Joaquín
Serrano, Álvaro
Tarifa, Rocío
Arroyo, Montserrat
Sorrentino, A.
Pereiro, Eva
Vázquez, Jesús
Valpuesta, José M.
Sánchez-Madrid, Francisco
Martín-Cófreces, Noa Beatriz
author Rojas-Gómez, Amelia
author_facet Rojas-Gómez, Amelia
Dosil, S. G.
Chichón, Francisco Javier
Fernández-Gallego, Nieves
Ferrarini, Alessia
Calvo, Enrique
Calzada-Fraile, Diego
Requena, Silvia
Otón, Joaquín
Serrano, Álvaro
Tarifa, Rocío
Arroyo, Montserrat
Sorrentino, A.
Pereiro, Eva
Vázquez, Jesús
Valpuesta, José M.
Sánchez-Madrid, Francisco
Martín-Cófreces, Noa Beatriz
author_role author
author2 Dosil, S. G.
Chichón, Francisco Javier
Fernández-Gallego, Nieves
Ferrarini, Alessia
Calvo, Enrique
Calzada-Fraile, Diego
Requena, Silvia
Otón, Joaquín
Serrano, Álvaro
Tarifa, Rocío
Arroyo, Montserrat
Sorrentino, A.
Pereiro, Eva
Vázquez, Jesús
Valpuesta, José M.
Sánchez-Madrid, Francisco
Martín-Cófreces, Noa Beatriz
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
Instituto de Salud Carlos III
Fundación la Caixa
Agencia Estatal de Investigación (España)
Rojas-Gómez, Amelia [0000-0003-0177-5644]
Dosil, Sara G [0000-0003-4548-4435]
Chichón, Francisco Javier [0000-0003-2766-0327]
Fernández-Gallego, Nieves [0000-0002-6968-1444]
Ferrarini, Alessia [0000-0002-4587-6095]
Calzada-Fraile, Diego [0000-0001-7259-0757]
Requena, Silvia [0000-0001-7332-7141]
Otón, Joaquin [0000-0002-2195-4730]
Pereiro, Eva [0000-0001-7626-5935]
Valpuesta, José M. [0000-0001-7468-8053]
Sánchez-Madrid, Francisco [0000-0001-5303-0762]
Martín-Cófreces, Noa B [0000-0003-0439-8979]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv CCT
chaperonin
extracellular vesicle
lipid droplet
lipidomic
peroxisome
topic CCT
chaperonin
extracellular vesicle
lipid droplet
lipidomic
peroxisome
description Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganelle contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/362902
https://api.elsevier.com/content/abstract/scopus_id/85162082519
url http://hdl.handle.net/10261/362902
https://api.elsevier.com/content/abstract/scopus_id/85162082519
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
S2022/BMD-7209/INTEGRAMUNE-CM
P2022/BMD-7333/INMUNOVAR-CM
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PLEC2022-009298
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097019-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122348NB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105872GB-I00
Journal of extracellular vesicles
application/pdf
https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.12333
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamicsRojas-Gómez, AmeliaDosil, S. G.Chichón, Francisco JavierFernández-Gallego, NievesFerrarini, AlessiaCalvo, EnriqueCalzada-Fraile, DiegoRequena, SilviaOtón, JoaquínSerrano, ÁlvaroTarifa, RocíoArroyo, MontserratSorrentino, A.Pereiro, EvaVázquez, JesúsValpuesta, José M.Sánchez-Madrid, FranciscoMartín-Cófreces, Noa BeatrizCCTchaperoninextracellular vesiclelipid dropletlipidomicperoxisomeCell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganelle contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.Comunidad de Madrid, Grant/Award Numbers: S2022/BMD-7209-INTEGRAMUNE-CM, P2022/BMD-7333; Carlos III Institute of Health-Fondo de Investigacion Sanitaria, Grant/Award Numbers: PRB3 (IPT17/0019 - ISCIII-SGEFI / ERDF), CIBER Cardiovascular (CIBERCV); La Caixa Banking Foundation, Grant/Award Numbers: HR17-00016, HR17-00247, HR22-00253, HR22-00722; consorcio para la construcción equipamiento y explotación del laboratorio de luz sincrotrón, Grant/Award Numbers: 2015021148, 2016021638; Agencia Estatal de Investigación, Grant/Award Numbers: PDC2021-121719-I00, PLEC2022-009298, PID-2020-120412RB-I00, PGC2018-097019-B-I00, PID2021-122348NB-I00, PID2019-105872GB- I00/AEI/10.13039/501100011033Peer reviewedJohn Wiley & SonsComunidad de MadridInstituto de Salud Carlos IIIFundación la CaixaAgencia Estatal de Investigación (España)Rojas-Gómez, Amelia [0000-0003-0177-5644]Dosil, Sara G [0000-0003-4548-4435]Chichón, Francisco Javier [0000-0003-2766-0327]Fernández-Gallego, Nieves [0000-0002-6968-1444]Ferrarini, Alessia [0000-0002-4587-6095]Calzada-Fraile, Diego [0000-0001-7259-0757]Requena, Silvia [0000-0001-7332-7141]Otón, Joaquin [0000-0002-2195-4730]Pereiro, Eva [0000-0001-7626-5935]Valpuesta, José M. [0000-0001-7468-8053]Sánchez-Madrid, Francisco [0000-0001-5303-0762]Martín-Cófreces, Noa B [0000-0003-0439-8979]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/362902https://api.elsevier.com/content/abstract/scopus_id/85162082519reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#S2022/BMD-7209/INTEGRAMUNE-CMP2022/BMD-7333/INMUNOVAR-CMinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PLEC2022-009298info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097019-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122348NB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105872GB-I00Journal of extracellular vesiclesapplication/pdfhttps://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.12333Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3629022026-05-22T06:33:51Z
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