X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk

X-linked Inhibitor of apoptosis protein (XIAP) has been classically identified as a cell death regulator. Here, we demonstrate a novel function of XIAP as a regulator of neurite outgrowth in neuronal cells. In PC12 cells, XIAP overexpression prevents NGF-induced neuronal differentiation, whereas NGF...

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Autores: Fadó, Rut|||0000-0002-3293-2342, Moubarak, Rana S.|||0000-0003-2096-1258, Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682, Barneda Zahonero, Bruna|||0000-0002-6557-5222, Valero Gomez-Lobo, Jorge, Saura Antolín, Carlos|||0000-0003-3692-5657, Morán, Julio, Comella i Carnicé, Joan Xavier|||0000-0002-6218-0786, Rodríguez Álvarez, José|||0000-0001-8582-8082
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:115507
Acceso en línea:https://ddd.uab.cat/record/115507
https://dx.doi.org/urn:doi:10.1038/srep02397
Access Level:acceso abierto
Palabra clave:Neurociència
Factors neurotròfics
Neurociència cel·lular
Creixement cel·lular
Neuroquímica
Neurotrophic factors
Cellular neuroscience
Cell growth
Neurochemistry
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network_acronym_str ES
network_name_str España
repository_id_str
spelling X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and TrkFadó, Rut|||0000-0002-3293-2342Moubarak, Rana S.|||0000-0003-2096-1258Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682Barneda Zahonero, Bruna|||0000-0002-6557-5222Valero Gomez-Lobo, JorgeSaura Antolín, Carlos|||0000-0003-3692-5657Morán, JulioComella i Carnicé, Joan Xavier|||0000-0002-6218-0786Rodríguez Álvarez, José|||0000-0001-8582-8082NeurociènciaFactors neurotròficsNeurociència cel·lularCreixement cel·lularNeuroquímicaNeurotrophic factorsCellular neuroscienceCell growthNeurochemistryX-linked Inhibitor of apoptosis protein (XIAP) has been classically identified as a cell death regulator. Here, we demonstrate a novel function of XIAP as a regulator of neurite outgrowth in neuronal cells. In PC12 cells, XIAP overexpression prevents NGF-induced neuronal differentiation, whereas NGF treatment induces a reduction of endogenous XIAP levels concomitant with the induction of neuronal differentiation. Accordingly, downregulation of endogenous XIAP protein levels strongly increases neurite outgrowth in PC12 cells as well as axonal and dendritic length in primary cortical neurons. The effects of XIAP are mediated by the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinases (ERKs) pathway since blocking this pathway completely prevents the neuritogenesis mediated by XIAP downregulation. In addition, we found that XIAP binds to cRaf and Trk receptors. Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons. 22013-01-0120132013-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/115507https://dx.doi.org/urn:doi:10.1038/srep02397reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 SAF2011-30281Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 SAF2010-19953Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 SAF2010-20925Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-1231Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-346open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/3.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1155072026-06-06T12:50:31Z
dc.title.none.fl_str_mv X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
title X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
spellingShingle X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
Fadó, Rut|||0000-0002-3293-2342
Neurociència
Factors neurotròfics
Neurociència cel·lular
Creixement cel·lular
Neuroquímica
Neurotrophic factors
Cellular neuroscience
Cell growth
Neurochemistry
title_short X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
title_full X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
title_fullStr X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
title_full_unstemmed X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
title_sort X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
dc.creator.none.fl_str_mv Fadó, Rut|||0000-0002-3293-2342
Moubarak, Rana S.|||0000-0003-2096-1258
Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682
Barneda Zahonero, Bruna|||0000-0002-6557-5222
Valero Gomez-Lobo, Jorge
Saura Antolín, Carlos|||0000-0003-3692-5657
Morán, Julio
Comella i Carnicé, Joan Xavier|||0000-0002-6218-0786
Rodríguez Álvarez, José|||0000-0001-8582-8082
author Fadó, Rut|||0000-0002-3293-2342
author_facet Fadó, Rut|||0000-0002-3293-2342
Moubarak, Rana S.|||0000-0003-2096-1258
Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682
Barneda Zahonero, Bruna|||0000-0002-6557-5222
Valero Gomez-Lobo, Jorge
Saura Antolín, Carlos|||0000-0003-3692-5657
Morán, Julio
Comella i Carnicé, Joan Xavier|||0000-0002-6218-0786
Rodríguez Álvarez, José|||0000-0001-8582-8082
author_role author
author2 Moubarak, Rana S.|||0000-0003-2096-1258
Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682
Barneda Zahonero, Bruna|||0000-0002-6557-5222
Valero Gomez-Lobo, Jorge
Saura Antolín, Carlos|||0000-0003-3692-5657
Morán, Julio
Comella i Carnicé, Joan Xavier|||0000-0002-6218-0786
Rodríguez Álvarez, José|||0000-0001-8582-8082
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Neurociència
Factors neurotròfics
Neurociència cel·lular
Creixement cel·lular
Neuroquímica
Neurotrophic factors
Cellular neuroscience
Cell growth
Neurochemistry
topic Neurociència
Factors neurotròfics
Neurociència cel·lular
Creixement cel·lular
Neuroquímica
Neurotrophic factors
Cellular neuroscience
Cell growth
Neurochemistry
description X-linked Inhibitor of apoptosis protein (XIAP) has been classically identified as a cell death regulator. Here, we demonstrate a novel function of XIAP as a regulator of neurite outgrowth in neuronal cells. In PC12 cells, XIAP overexpression prevents NGF-induced neuronal differentiation, whereas NGF treatment induces a reduction of endogenous XIAP levels concomitant with the induction of neuronal differentiation. Accordingly, downregulation of endogenous XIAP protein levels strongly increases neurite outgrowth in PC12 cells as well as axonal and dendritic length in primary cortical neurons. The effects of XIAP are mediated by the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinases (ERKs) pathway since blocking this pathway completely prevents the neuritogenesis mediated by XIAP downregulation. In addition, we found that XIAP binds to cRaf and Trk receptors. Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons.
publishDate 2013
dc.date.none.fl_str_mv 2
2013-01-01
2013
2013-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/115507
https://dx.doi.org/urn:doi:10.1038/srep02397
url https://ddd.uab.cat/record/115507
https://dx.doi.org/urn:doi:10.1038/srep02397
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 SAF2011-30281
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 SAF2010-19953
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 SAF2010-20925
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-1231
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-346
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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