Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications

The thrombin binding aptamer (TBA) is a well characterized chair-like, antiparallel quadruplex structure that binds specifically to thrombin at nanomolar concentrations and therefore it has interesting anticoagulant properties. In this article we review the research involved in the development of ne...

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Detalles Bibliográficos
Autores: Aviñó, Anna, Eritja Casadellà, Ramón, Fàbrega, Carme, Tintoré, María
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/125219
Acceso en línea:http://hdl.handle.net/10261/125219
Access Level:acceso abierto
Palabra clave:Anticoagulants
Aptamers
DNA
G-quadruplex
TBA
Oligonucleotide synthesis
Thrombin binding aptamer
Thrombin
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spelling Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical ApplicationsAviñó, AnnaEritja Casadellà, RamónFàbrega, CarmeTintoré, MaríaAnticoagulantsAptamersDNAG-quadruplexTBAOligonucleotide synthesisThrombin binding aptamerThrombinThe thrombin binding aptamer (TBA) is a well characterized chair-like, antiparallel quadruplex structure that binds specifically to thrombin at nanomolar concentrations and therefore it has interesting anticoagulant properties. In this article we review the research involved in the development of new TBA derivatives with improved anticoagulant properties as well as the use of the TBA as a model compound for the study of quadruplex structures. Specifically, we describe the impact of modified nucleosides and non-natural backbones in the guanine tetrads or in the loops and the introduction of pendant groups at the 3' or 5'-ends. The modified oligonucleotides are shown to be excellent tools for the understanding of the molecular structure of the TBA and its folding properties. Finally, we review the use of the TBA-Thrombin recognition system for the development of analytical tools based on the TBA folding.This study was supported by the European Communities (FUNMOL, FP7-NMP- 213382-2), Spanish Ministry of Education (MOL2MED, CTQ2010-20541), the Generalitat de Catalunya (2009/SGR/208), IRB Barcelona, COST (G4net, MP0802) and CIBER-BBN (VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions, Instituto de Salud Carlos III with assistance from the European Regional Development Fund.NoBentham Science PublishersConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/125219reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDOI: 10.2174/138161212799958387Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1252192026-05-22T06:33:51Z
dc.title.none.fl_str_mv Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
title Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
spellingShingle Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
Aviñó, Anna
Anticoagulants
Aptamers
DNA
G-quadruplex
TBA
Oligonucleotide synthesis
Thrombin binding aptamer
Thrombin
title_short Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
title_full Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
title_fullStr Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
title_full_unstemmed Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
title_sort Thrombin Binding Aptamer, more than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
dc.creator.none.fl_str_mv Aviñó, Anna
Eritja Casadellà, Ramón
Fàbrega, Carme
Tintoré, María
author Aviñó, Anna
author_facet Aviñó, Anna
Eritja Casadellà, Ramón
Fàbrega, Carme
Tintoré, María
author_role author
author2 Eritja Casadellà, Ramón
Fàbrega, Carme
Tintoré, María
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anticoagulants
Aptamers
DNA
G-quadruplex
TBA
Oligonucleotide synthesis
Thrombin binding aptamer
Thrombin
topic Anticoagulants
Aptamers
DNA
G-quadruplex
TBA
Oligonucleotide synthesis
Thrombin binding aptamer
Thrombin
description The thrombin binding aptamer (TBA) is a well characterized chair-like, antiparallel quadruplex structure that binds specifically to thrombin at nanomolar concentrations and therefore it has interesting anticoagulant properties. In this article we review the research involved in the development of new TBA derivatives with improved anticoagulant properties as well as the use of the TBA as a model compound for the study of quadruplex structures. Specifically, we describe the impact of modified nucleosides and non-natural backbones in the guanine tetrads or in the loops and the introduction of pendant groups at the 3' or 5'-ends. The modified oligonucleotides are shown to be excellent tools for the understanding of the molecular structure of the TBA and its folding properties. Finally, we review the use of the TBA-Thrombin recognition system for the development of analytical tools based on the TBA folding.
publishDate 2012
dc.date.none.fl_str_mv 2012
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/125219
url http://hdl.handle.net/10261/125219
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv DOI: 10.2174/138161212799958387
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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