Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer

This work reports a dual immunoplatform for the simultaneous detection of two epithelial glycoproteins of the mucin family, mucin 1 (MUC1) and mucin 16 (MUC16), whose expression is related to adverse prognosis and minimal residual disease (MRD) in colorectal cancer (CRC). The developed immunoplatfor...

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Authors: Tejerina Miranda, Sandra, Blázquez-García, Marina, Serafín González-Carrato, Verónica, Montero-Calle, Ana, Garranzo-Asensio, Maria, Reviejo García, Ángel Julio, Pedrero Muñoz, María, Pingarrón Carrazón, José Manuel, Barderas Manchado, Rodrigo, Campuzano Ruiz, Susana
Format: article
Publication Date:2023
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/113814
Online Access:https://hdl.handle.net/20.500.14352/113814
Access Level:Open access
Keyword:543
Electrochemical immunoplatforms
Mucins
Colorectal cancer
Aggressiveness
cancer cells
Ciencias
2301 Química Analítica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/113814
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network_name_str España
repository_id_str
spelling Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancerTejerina Miranda, SandraBlázquez-García, MarinaSerafín González-Carrato, VerónicaMontero-Calle, AnaGarranzo-Asensio, MariaReviejo García, Ángel JulioPedrero Muñoz, MaríaPingarrón Carrazón, José ManuelBarderas Manchado, RodrigoCampuzano Ruiz, Susana543Electrochemical immunoplatformsMucinsColorectal cancerAggressivenesscancer cellsCiencias2301 Química AnalíticaThis work reports a dual immunoplatform for the simultaneous detection of two epithelial glycoproteins of the mucin family, mucin 1 (MUC1) and mucin 16 (MUC16), whose expression is related to adverse prognosis and minimal residual disease (MRD) in colorectal cancer (CRC). The developed immunoplatform involves functionalised magnetic microparticles (MBs), a set of specific antibody pairs (a capture antibody, cAb, and a biotinylated detector antibody b-dAb labelled with a streptavidin-horseradish peroxidase, Strep-HRP, polymer) for each target protein and amperometric detection at dual screen-printed carbon electrodes (SPdCEs) using the hydroquinone (HQ)/horseradish peroxidase (HRP)/H2O2 system. This dual immunoplatform allows, under the optimised experimental conditions, to achieve LOD values of 50 and 1.81 pg mL−1 (or mU mL−1) for MUC1 and MUC16, respectively, and adequate selectivity for the determination of the two targets in the clinic. The developed immunoplatform was employed to analyse CRC cell protein extracts (1.0 μg/determination) with different metastatic potential providing results in agreement with those obtained by blotting technologies but using affordable and applicable point-of-care instruments. This new biotool also emerges competitive in state-of-the-art electrochemical immunoplatforms seeking a compromise among simplicity, reduction of test time and analytical characteristics.ElsevierUniversidad Complutense de Madrid20232023-01-0120232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/113814reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1138142026-06-02T12:44:21Z
dc.title.none.fl_str_mv Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer
title Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer
spellingShingle Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer
Tejerina Miranda, Sandra
543
Electrochemical immunoplatforms
Mucins
Colorectal cancer
Aggressiveness
cancer cells
Ciencias
2301 Química Analítica
title_short Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer
title_full Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer
title_fullStr Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer
title_full_unstemmed Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer
title_sort Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer
dc.creator.none.fl_str_mv Tejerina Miranda, Sandra
Blázquez-García, Marina
Serafín González-Carrato, Verónica
Montero-Calle, Ana
Garranzo-Asensio, Maria
Reviejo García, Ángel Julio
Pedrero Muñoz, María
Pingarrón Carrazón, José Manuel
Barderas Manchado, Rodrigo
Campuzano Ruiz, Susana
author Tejerina Miranda, Sandra
author_facet Tejerina Miranda, Sandra
Blázquez-García, Marina
Serafín González-Carrato, Verónica
Montero-Calle, Ana
Garranzo-Asensio, Maria
Reviejo García, Ángel Julio
Pedrero Muñoz, María
Pingarrón Carrazón, José Manuel
Barderas Manchado, Rodrigo
Campuzano Ruiz, Susana
author_role author
author2 Blázquez-García, Marina
Serafín González-Carrato, Verónica
Montero-Calle, Ana
Garranzo-Asensio, Maria
Reviejo García, Ángel Julio
Pedrero Muñoz, María
Pingarrón Carrazón, José Manuel
Barderas Manchado, Rodrigo
Campuzano Ruiz, Susana
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Electrochemical immunoplatforms
Mucins
Colorectal cancer
Aggressiveness
cancer cells
Ciencias
2301 Química Analítica
topic 543
Electrochemical immunoplatforms
Mucins
Colorectal cancer
Aggressiveness
cancer cells
Ciencias
2301 Química Analítica
description This work reports a dual immunoplatform for the simultaneous detection of two epithelial glycoproteins of the mucin family, mucin 1 (MUC1) and mucin 16 (MUC16), whose expression is related to adverse prognosis and minimal residual disease (MRD) in colorectal cancer (CRC). The developed immunoplatform involves functionalised magnetic microparticles (MBs), a set of specific antibody pairs (a capture antibody, cAb, and a biotinylated detector antibody b-dAb labelled with a streptavidin-horseradish peroxidase, Strep-HRP, polymer) for each target protein and amperometric detection at dual screen-printed carbon electrodes (SPdCEs) using the hydroquinone (HQ)/horseradish peroxidase (HRP)/H2O2 system. This dual immunoplatform allows, under the optimised experimental conditions, to achieve LOD values of 50 and 1.81 pg mL−1 (or mU mL−1) for MUC1 and MUC16, respectively, and adequate selectivity for the determination of the two targets in the clinic. The developed immunoplatform was employed to analyse CRC cell protein extracts (1.0 μg/determination) with different metastatic potential providing results in agreement with those obtained by blotting technologies but using affordable and applicable point-of-care instruments. This new biotool also emerges competitive in state-of-the-art electrochemical immunoplatforms seeking a compromise among simplicity, reduction of test time and analytical characteristics.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/113814
url https://hdl.handle.net/20.500.14352/113814
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423004670230528
score 15.812429

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