CriTER-A: A Novel Temperature-Dependent Noncoding RNA Switch in the Telomeric Transcriptome of Chironomus riparius
The telomeric transcriptome of Chironomus riparius has been involved in thermal stress response. One of the telomeric transcripts, the so¿called CriTER¿A variant, is highly overexpressed upon heat shock. On the other hand, its homologous variant CriTER¿B, which is the most frequently encoded noncodi...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/358616 |
| Acceso en línea: | http://hdl.handle.net/10261/358616 |
| Access Level: | acceso abierto |
| Palabra clave: | telomeric noncoding RNAs RNA thermo-switch RNA structure prediction conformational analysis SHAPE analysis |
| Sumario: | The telomeric transcriptome of Chironomus riparius has been involved in thermal stress response. One of the telomeric transcripts, the so¿called CriTER¿A variant, is highly overexpressed upon heat shock. On the other hand, its homologous variant CriTER¿B, which is the most frequently encoded noncoding RNA in the telomeres of C. riparius, is only slightly affected by thermal stress. Interestingly, both transcripts show high sequence homology, but less is known about their folding and how this could influence their differential behaviour. Our study suggests that CriTER¿A folds as two different conformers, whose relative proportion is influenced by temperature conditions. Meanwhile, the CriTER¿B variant shows only one dominant conformer. Thus, a temperature¿de-pendent conformational equilibrium can be established for CriTER¿A, suggesting a putative functional role of the telomeric transcriptome in relation to thermal stress that could rely on the struc-ture¿function relationship of the CriTER¿A transcripts. |
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