Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2

Although adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 could respecify SEZ neural stem cells and their progeny toward a...

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Autores: Péron, Sophie, Miyakoshi, Leo M., Brill, Monika S., Manzano Franco, Diana, Serrano López, Julia, Fan, Wenqiang, Marichal, Nicolás, Ghanem, Alexander, Conzelmann, Karl-Klaus, Karow, Marisa, Ortega De La O, Felipe, Gascón Jiménez, Sergio, Berninger, Benedikt
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/104218
Acceso en línea:https://hdl.handle.net/20.500.14352/104218
Access Level:acceso abierto
Palabra clave:577.1/2
Adult neurogenesis
Neural specification
Neural stem cell
Neurogenin 2
Adult subventricular zone
Olfactory bulb
Fate reprogramming
Proneural gene
Bioquímica (Biología)
Biología molecular (Biología)
2403 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/104218
network_acronym_str ES
network_name_str España
repository_id_str
spelling Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2Péron, SophieMiyakoshi, Leo M.Brill, Monika S.Manzano Franco, DianaSerrano López, JuliaFan, WenqiangMarichal, NicolásGhanem, AlexanderConzelmann, Karl-KlausKarow, MarisaOrtega De La O, FelipeGascón Jiménez, SergioBerninger, Benedikt577.1/2Adult neurogenesisNeural specificationNeural stem cellNeurogenin 2Adult subventricular zoneOlfactory bulbFate reprogrammingProneural geneBioquímica (Biología)Biología molecular (Biología)2403 BioquímicaAlthough adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 could respecify SEZ neural stem cells and their progeny toward a glutamatergic fate. Retrovirus-mediated expression of Neurog2 induced the glutamatergic lineage markers TBR2 and TBR1 in cultured SEZ progenitors, which differentiated into functional glutamatergic neurons. Likewise, Neurog2-transduced SEZ progenitors acquired glutamatergic neuron hallmarks in vivo. Intriguingly, they failed to migrate toward the olfactory bulb and instead differentiated within the SEZ or the adjacent striatum, where they received connections from local neurons, as indicated by rabies virus-mediated monosynaptic tracing. In contrast, lentivirus-mediated expression of Neurog2 failed to reprogram early SEZ neurons, which maintained GABAergic identity and migrated to the olfactory bulb. Our data show that NEUROG2 can program SEZ progenitors toward a glutamatergic identity but fails to reprogram their neuronal progeny.Cell PressUniversidad Complutense de Madrid20232023-12-1220232023-12-12journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/104218reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1042182026-06-02T12:44:21Z
dc.title.none.fl_str_mv Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
title Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
spellingShingle Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
Péron, Sophie
577.1/2
Adult neurogenesis
Neural specification
Neural stem cell
Neurogenin 2
Adult subventricular zone
Olfactory bulb
Fate reprogramming
Proneural gene
Bioquímica (Biología)
Biología molecular (Biología)
2403 Bioquímica
title_short Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
title_full Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
title_fullStr Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
title_full_unstemmed Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
title_sort Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
dc.creator.none.fl_str_mv Péron, Sophie
Miyakoshi, Leo M.
Brill, Monika S.
Manzano Franco, Diana
Serrano López, Julia
Fan, Wenqiang
Marichal, Nicolás
Ghanem, Alexander
Conzelmann, Karl-Klaus
Karow, Marisa
Ortega De La O, Felipe
Gascón Jiménez, Sergio
Berninger, Benedikt
author Péron, Sophie
author_facet Péron, Sophie
Miyakoshi, Leo M.
Brill, Monika S.
Manzano Franco, Diana
Serrano López, Julia
Fan, Wenqiang
Marichal, Nicolás
Ghanem, Alexander
Conzelmann, Karl-Klaus
Karow, Marisa
Ortega De La O, Felipe
Gascón Jiménez, Sergio
Berninger, Benedikt
author_role author
author2 Miyakoshi, Leo M.
Brill, Monika S.
Manzano Franco, Diana
Serrano López, Julia
Fan, Wenqiang
Marichal, Nicolás
Ghanem, Alexander
Conzelmann, Karl-Klaus
Karow, Marisa
Ortega De La O, Felipe
Gascón Jiménez, Sergio
Berninger, Benedikt
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.1/2
Adult neurogenesis
Neural specification
Neural stem cell
Neurogenin 2
Adult subventricular zone
Olfactory bulb
Fate reprogramming
Proneural gene
Bioquímica (Biología)
Biología molecular (Biología)
2403 Bioquímica
topic 577.1/2
Adult neurogenesis
Neural specification
Neural stem cell
Neurogenin 2
Adult subventricular zone
Olfactory bulb
Fate reprogramming
Proneural gene
Bioquímica (Biología)
Biología molecular (Biología)
2403 Bioquímica
description Although adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 could respecify SEZ neural stem cells and their progeny toward a glutamatergic fate. Retrovirus-mediated expression of Neurog2 induced the glutamatergic lineage markers TBR2 and TBR1 in cultured SEZ progenitors, which differentiated into functional glutamatergic neurons. Likewise, Neurog2-transduced SEZ progenitors acquired glutamatergic neuron hallmarks in vivo. Intriguingly, they failed to migrate toward the olfactory bulb and instead differentiated within the SEZ or the adjacent striatum, where they received connections from local neurons, as indicated by rabies virus-mediated monosynaptic tracing. In contrast, lentivirus-mediated expression of Neurog2 failed to reprogram early SEZ neurons, which maintained GABAergic identity and migrated to the olfactory bulb. Our data show that NEUROG2 can program SEZ progenitors toward a glutamatergic identity but fails to reprogram their neuronal progeny.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-12-12
2023
2023-12-12
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/104218
url https://hdl.handle.net/20.500.14352/104218
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422992100950016
score 15,300724

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