Dynamics of cell death after conventional IRE and H-FIRE treatments

High-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are r...

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Detalles Bibliográficos
Autores: Mercadal, Borja, Beitel White, Natalie, Aycock, Kenneth N., Castellví Fernández, Quim, Davalos, Rafael Vidal, Ivorra Cano, Antoni, 1974-
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/43888
Acceso en línea:http://hdl.handle.net/10230/43888
http://dx.doi.org/10.1007/s10439-020-02462-8
Access Level:acceso abierto
Palabra clave:Irreversible electroporation
High-frequency irreversible electroporation
Membrane permeability
Caspase 3/7
Bipolar pulses
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spelling Dynamics of cell death after conventional IRE and H-FIRE treatmentsMercadal, BorjaBeitel White, NatalieAycock, Kenneth N.Castellví Fernández, QuimDavalos, Rafael VidalIvorra Cano, Antoni, 1974-Irreversible electroporationHigh-frequency irreversible electroporationMembrane permeabilityCaspase 3/7Bipolar pulsesHigh-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are replaced with bursts of short bipolar pulses. Currently, very little is known regarding how the use of a different waveform affects the cell death dynamics and mechanisms. In this study, human pancreatic adenocarcinoma cells were treated with a typical IRE protocol and various H-FIRE schemes with the same energized time. Cell viability, membrane integrity and Caspase 3/7 activity were assessed at different times after the treatment. In both treatments, we identified two different death dynamics (immediate and delayed) and we quantified the electric field ranges that lead to each of them. While in the typical IRE protocol, the electric field range leading to a delayed cell death is very narrow, this range is wider in H-FIRE and can be increased by reducing the pulse length. Membrane integrity in cells suffering a delayed cell death shows a similar time evolution in all treatments, however, Caspase 3/7 expression was only observed in cells treated with H-FIRE.This work was supported by the Ministry of Economy and Competitiveness of Spain (Grant No. TEC2014-52383-C3-2-R), the Pancreatic Cancer Action Network (Grant No. 16-65-IANN) and Cures Within Reach (Grant No. PUJFSANY). Antoni Ivorra gratefully acknowledges financial support by ICREA under the ICREA Academia programme.Springer202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/43888http://dx.doi.org/10.1007/s10439-020-02462-8reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésAnnals of Biomedical Engineering. 2020info:eu-repo/grantAgreement/ES/1PE/TEC2014-52383-C3-2-RThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/438882026-05-29T05:05:01Z
dc.title.none.fl_str_mv Dynamics of cell death after conventional IRE and H-FIRE treatments
title Dynamics of cell death after conventional IRE and H-FIRE treatments
spellingShingle Dynamics of cell death after conventional IRE and H-FIRE treatments
Mercadal, Borja
Irreversible electroporation
High-frequency irreversible electroporation
Membrane permeability
Caspase 3/7
Bipolar pulses
title_short Dynamics of cell death after conventional IRE and H-FIRE treatments
title_full Dynamics of cell death after conventional IRE and H-FIRE treatments
title_fullStr Dynamics of cell death after conventional IRE and H-FIRE treatments
title_full_unstemmed Dynamics of cell death after conventional IRE and H-FIRE treatments
title_sort Dynamics of cell death after conventional IRE and H-FIRE treatments
dc.creator.none.fl_str_mv Mercadal, Borja
Beitel White, Natalie
Aycock, Kenneth N.
Castellví Fernández, Quim
Davalos, Rafael Vidal
Ivorra Cano, Antoni, 1974-
author Mercadal, Borja
author_facet Mercadal, Borja
Beitel White, Natalie
Aycock, Kenneth N.
Castellví Fernández, Quim
Davalos, Rafael Vidal
Ivorra Cano, Antoni, 1974-
author_role author
author2 Beitel White, Natalie
Aycock, Kenneth N.
Castellví Fernández, Quim
Davalos, Rafael Vidal
Ivorra Cano, Antoni, 1974-
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Irreversible electroporation
High-frequency irreversible electroporation
Membrane permeability
Caspase 3/7
Bipolar pulses
topic Irreversible electroporation
High-frequency irreversible electroporation
Membrane permeability
Caspase 3/7
Bipolar pulses
description High-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are replaced with bursts of short bipolar pulses. Currently, very little is known regarding how the use of a different waveform affects the cell death dynamics and mechanisms. In this study, human pancreatic adenocarcinoma cells were treated with a typical IRE protocol and various H-FIRE schemes with the same energized time. Cell viability, membrane integrity and Caspase 3/7 activity were assessed at different times after the treatment. In both treatments, we identified two different death dynamics (immediate and delayed) and we quantified the electric field ranges that lead to each of them. While in the typical IRE protocol, the electric field range leading to a delayed cell death is very narrow, this range is wider in H-FIRE and can be increased by reducing the pulse length. Membrane integrity in cells suffering a delayed cell death shows a similar time evolution in all treatments, however, Caspase 3/7 expression was only observed in cells treated with H-FIRE.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/43888
http://dx.doi.org/10.1007/s10439-020-02462-8
url http://hdl.handle.net/10230/43888
http://dx.doi.org/10.1007/s10439-020-02462-8
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Annals of Biomedical Engineering. 2020
info:eu-repo/grantAgreement/ES/1PE/TEC2014-52383-C3-2-R
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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