Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit
[EN] Although the effects of sperm microbiota and sperm quality have been described previously, recent studies provide evidence that female genital modifications triggered by seminal components could be of significant importance to identify some disturbances associated with fertility. So, sperm micr...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/176314 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/176314 |
| Access Level: | acceso abierto |
| Palabra clave: | Microbiota 16S rRNA Semen Fertility Inbred line BIOLOGIA ANIMAL PRODUCCION ANIMAL |
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Roles of host genetics and sperm microbiota in reproductive success in healthy rabbitMarco-Jiménez, Francisco|||0000-0002-5226-4118Vicente Antón, José Salvador|||0000-0003-0481-3802Borrás-Pérez, SaraGarcia-Dominguez, XD Auria, GiuseppeMarin, ClaraMicrobiota16S rRNASemenFertilityInbred lineBIOLOGIA ANIMALPRODUCCION ANIMAL[EN] Although the effects of sperm microbiota and sperm quality have been described previously, recent studies provide evidence that female genital modifications triggered by seminal components could be of significant importance to identify some disturbances associated with fertility. So, sperm microbiota could play a key role in sperm quality, contributing to fertilisation. To understand how sperm microbiota diversity is influenced by the host genetics, the symbiotic bacteria in four inbred lines raised in the same animal facility and their effects on sperm quality and fertility were analysed. Forty healthy rabbits from four selected Spanish commercial lines were used in this research (three based on litter performance, designated A, V and LP, and one selected for daily body weight gain, called R). Significant variations in the seminal concentration, morphology and some motion parameters were found among inbred lines, but sperm motility and viability were similar among inbred lines. After mating, inbred lines selected for litter size had the same fertility rate, significantly higher than inbred line selected for body weight (82 ± 3.3%, 79 ± 3.5% and 89 ± 4.5% versus 61 ± 3.7%, for the A, V and LP vs R lines, respectively, p < 0.05). Bacteria belonging to Proteobacteria, Firmicutes, Fusobacteria and Bacteroidetes were identified in sperm microbiota. At genus level, the bacterial community composition in the sperm microbiota was influenced by host genetics. A total of 35, 16, 34, and 51 genera were accurately detected in the A, V, LP, and R lines, respectively. Moreover, Enhydrobacter, Ferruginibacter, Myroides Paracoccus, Rheinheimera, Tepidiphilus, Tetradesmus obliquus and Thauera genera were present only in the inbred lines selected for litter size. Moreover, the discriminant analysis revealed Lysinibacillus and Flavobacterium genera as potential biomarkers for fertility. Thus, these two genera may play a key role in fertility. Our results demonstrated the existence of a rabbit inbred line-specific variation in bacterial occurrence in sperm microbiota. Moreover, fertility differentials among inbred lines that were not predicted by routine semen analysis could be partly explained by the symbiotic state of the semen microbiota.Funding from the Ministry of Economy, Industry and Competitiveness (Research project: AGL2017-85162-C2-1-R) is acknowledged. X.G.D. was supported by a research grant from the Ministry of Economy, Industry and Competitiveness (BES-2015-072429). English text version was revised by N. Macowan English Language Service.ElsevierDepartamento de Ciencia AnimalInstituto Universitario de Ciencia y Tecnología AnimalEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalAGENCIA ESTATAL DE INVESTIGACIONRepositorio Institucional de la Universitat Politècnica de València Riunet20202020-12-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/176314reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 AGL2017-85162-C2-1-R MEJORA GENETICA DEL CONEJO DE CARNE: ESTRATEGIAS PARA INCREMENTAR LA EFICACIA DE LA MEJORA, REPRODUCCION Y SALUD DE LINEAS PATERNALESopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1763142026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit |
| title |
Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit |
| spellingShingle |
Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit Marco-Jiménez, Francisco|||0000-0002-5226-4118 Microbiota 16S rRNA Semen Fertility Inbred line BIOLOGIA ANIMAL PRODUCCION ANIMAL |
| title_short |
Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit |
| title_full |
Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit |
| title_fullStr |
Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit |
| title_full_unstemmed |
Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit |
| title_sort |
Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit |
| dc.creator.none.fl_str_mv |
Marco-Jiménez, Francisco|||0000-0002-5226-4118 Vicente Antón, José Salvador|||0000-0003-0481-3802 Borrás-Pérez, Sara Garcia-Dominguez, X D Auria, Giuseppe Marin, Clara |
| author |
Marco-Jiménez, Francisco|||0000-0002-5226-4118 |
| author_facet |
Marco-Jiménez, Francisco|||0000-0002-5226-4118 Vicente Antón, José Salvador|||0000-0003-0481-3802 Borrás-Pérez, Sara Garcia-Dominguez, X D Auria, Giuseppe Marin, Clara |
| author_role |
author |
| author2 |
Vicente Antón, José Salvador|||0000-0003-0481-3802 Borrás-Pérez, Sara Garcia-Dominguez, X D Auria, Giuseppe Marin, Clara |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Ciencia Animal Instituto Universitario de Ciencia y Tecnología Animal Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural AGENCIA ESTATAL DE INVESTIGACION Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Microbiota 16S rRNA Semen Fertility Inbred line BIOLOGIA ANIMAL PRODUCCION ANIMAL |
| topic |
Microbiota 16S rRNA Semen Fertility Inbred line BIOLOGIA ANIMAL PRODUCCION ANIMAL |
| description |
[EN] Although the effects of sperm microbiota and sperm quality have been described previously, recent studies provide evidence that female genital modifications triggered by seminal components could be of significant importance to identify some disturbances associated with fertility. So, sperm microbiota could play a key role in sperm quality, contributing to fertilisation. To understand how sperm microbiota diversity is influenced by the host genetics, the symbiotic bacteria in four inbred lines raised in the same animal facility and their effects on sperm quality and fertility were analysed. Forty healthy rabbits from four selected Spanish commercial lines were used in this research (three based on litter performance, designated A, V and LP, and one selected for daily body weight gain, called R). Significant variations in the seminal concentration, morphology and some motion parameters were found among inbred lines, but sperm motility and viability were similar among inbred lines. After mating, inbred lines selected for litter size had the same fertility rate, significantly higher than inbred line selected for body weight (82 ± 3.3%, 79 ± 3.5% and 89 ± 4.5% versus 61 ± 3.7%, for the A, V and LP vs R lines, respectively, p < 0.05). Bacteria belonging to Proteobacteria, Firmicutes, Fusobacteria and Bacteroidetes were identified in sperm microbiota. At genus level, the bacterial community composition in the sperm microbiota was influenced by host genetics. A total of 35, 16, 34, and 51 genera were accurately detected in the A, V, LP, and R lines, respectively. Moreover, Enhydrobacter, Ferruginibacter, Myroides Paracoccus, Rheinheimera, Tepidiphilus, Tetradesmus obliquus and Thauera genera were present only in the inbred lines selected for litter size. Moreover, the discriminant analysis revealed Lysinibacillus and Flavobacterium genera as potential biomarkers for fertility. Thus, these two genera may play a key role in fertility. Our results demonstrated the existence of a rabbit inbred line-specific variation in bacterial occurrence in sperm microbiota. Moreover, fertility differentials among inbred lines that were not predicted by routine semen analysis could be partly explained by the symbiotic state of the semen microbiota. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-12-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/176314 |
| url |
https://riunet.upv.es/handle/10251/176314 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 AGL2017-85162-C2-1-R MEJORA GENETICA DEL CONEJO DE CARNE: ESTRATEGIAS PARA INCREMENTAR LA EFICACIA DE LA MEJORA, REPRODUCCION Y SALUD DE LINEAS PATERNALES |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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Universitat Politècnica de València (UPV) |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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