Regional differences in the expression of laminin isoforms during mouse neural tube development

Many significant human birth defects originate around the time of neural tube closure or early during post-closure nervous system development. For example, failure of the neural tube to close generates anencephaly and spina bifida, faulty cell cycle progression is implicated in primary microcephaly,...

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Autores: Copp, Andrew J., Carvalho, Rita, Wallace, Adam, Sorokin, Lydia, Sasaki, Takako, Greene, Nicholas D. E., Ybot, Patricia
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/348085
Acceso en línea:http://hdl.handle.net/10261/348085
https://api.elsevier.com/content/abstract/scopus_id/79958093337
Access Level:acceso abierto
Palabra clave:Neural tube defects
Laminin
Mouse embryo
Neural tube closure
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spelling Regional differences in the expression of laminin isoforms during mouse neural tube developmentCopp, Andrew J.Carvalho, RitaWallace, AdamSorokin, LydiaSasaki, TakakoGreene, Nicholas D. E.Ybot, PatriciaNeural tube defectsLamininMouse embryoNeural tube closureMany significant human birth defects originate around the time of neural tube closure or early during post-closure nervous system development. For example, failure of the neural tube to close generates anencephaly and spina bifida, faulty cell cycle progression is implicated in primary microcephaly, while defective migration of neuroblasts can lead to neuronal migration disorders such as lissencephaly. At the stage of neural tube closure, basement membranes are becoming organised around the neuroepithelium, and beneath the adjacent non-neural surface ectoderm. While there is circumstantial evidence to implicate basement membrane dynamics in neural tube and surface ectodermal development, we have an incomplete understanding of the molecular composition of basement membranes at this stage. In the present study, we examined the developing basement membranes of the mouse embryo at mid-gestation (embryonic day 9.5), with particular reference to laminin composition. We performed in situ hybridization to detect the mRNAs of all eleven individual laminin chains, and immunohistochemistry to identify which laminin chains are present in the basement membranes. From this information, we inferred the likely laminin variants and their tissues of origin: that is, whether a given basement membrane laminin is contributed by epithelium, mesenchyme, or both. Our findings reveal major differences in basement composition along the body axis, with the rostral neural tube (at mandibular arch and heart levels) exhibiting many distinct laminin variants, while the lumbar level where the neural tube is just closing shows a much simpler laminin profile. Moreover, there appears to be a marked difference in the extent to which the mesenchyme contributes laminin variants to the basement membrane, with potential contribution of several laminins rostrally, but no contribution caudally. This information paves the way towards a mechanistic analysis of basement membrane laminin function during early neural tube development in mammals.The authors gratefully acknowledge financial support for this work by the de Instituto de Salud Carlos III project CP08/00111 and PS09/00050 (to PYG), and by Wellcome Trust grant 06883 (to AJC and NDG).Peer reviewedElsevierInstituto de Salud Carlos IIIWellcome TrustConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/348085https://api.elsevier.com/content/abstract/scopus_id/79958093337reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.matbio.2011.04.001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3480852026-05-22T06:33:51Z
dc.title.none.fl_str_mv Regional differences in the expression of laminin isoforms during mouse neural tube development
title Regional differences in the expression of laminin isoforms during mouse neural tube development
spellingShingle Regional differences in the expression of laminin isoforms during mouse neural tube development
Copp, Andrew J.
Neural tube defects
Laminin
Mouse embryo
Neural tube closure
title_short Regional differences in the expression of laminin isoforms during mouse neural tube development
title_full Regional differences in the expression of laminin isoforms during mouse neural tube development
title_fullStr Regional differences in the expression of laminin isoforms during mouse neural tube development
title_full_unstemmed Regional differences in the expression of laminin isoforms during mouse neural tube development
title_sort Regional differences in the expression of laminin isoforms during mouse neural tube development
dc.creator.none.fl_str_mv Copp, Andrew J.
Carvalho, Rita
Wallace, Adam
Sorokin, Lydia
Sasaki, Takako
Greene, Nicholas D. E.
Ybot, Patricia
author Copp, Andrew J.
author_facet Copp, Andrew J.
Carvalho, Rita
Wallace, Adam
Sorokin, Lydia
Sasaki, Takako
Greene, Nicholas D. E.
Ybot, Patricia
author_role author
author2 Carvalho, Rita
Wallace, Adam
Sorokin, Lydia
Sasaki, Takako
Greene, Nicholas D. E.
Ybot, Patricia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Wellcome Trust
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Neural tube defects
Laminin
Mouse embryo
Neural tube closure
topic Neural tube defects
Laminin
Mouse embryo
Neural tube closure
description Many significant human birth defects originate around the time of neural tube closure or early during post-closure nervous system development. For example, failure of the neural tube to close generates anencephaly and spina bifida, faulty cell cycle progression is implicated in primary microcephaly, while defective migration of neuroblasts can lead to neuronal migration disorders such as lissencephaly. At the stage of neural tube closure, basement membranes are becoming organised around the neuroepithelium, and beneath the adjacent non-neural surface ectoderm. While there is circumstantial evidence to implicate basement membrane dynamics in neural tube and surface ectodermal development, we have an incomplete understanding of the molecular composition of basement membranes at this stage. In the present study, we examined the developing basement membranes of the mouse embryo at mid-gestation (embryonic day 9.5), with particular reference to laminin composition. We performed in situ hybridization to detect the mRNAs of all eleven individual laminin chains, and immunohistochemistry to identify which laminin chains are present in the basement membranes. From this information, we inferred the likely laminin variants and their tissues of origin: that is, whether a given basement membrane laminin is contributed by epithelium, mesenchyme, or both. Our findings reveal major differences in basement composition along the body axis, with the rostral neural tube (at mandibular arch and heart levels) exhibiting many distinct laminin variants, while the lumbar level where the neural tube is just closing shows a much simpler laminin profile. Moreover, there appears to be a marked difference in the extent to which the mesenchyme contributes laminin variants to the basement membrane, with potential contribution of several laminins rostrally, but no contribution caudally. This information paves the way towards a mechanistic analysis of basement membrane laminin function during early neural tube development in mammals.
publishDate 2011
dc.date.none.fl_str_mv 2011
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/348085
https://api.elsevier.com/content/abstract/scopus_id/79958093337
url http://hdl.handle.net/10261/348085
https://api.elsevier.com/content/abstract/scopus_id/79958093337
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.matbio.2011.04.001
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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