Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation

Electrical stimulation (ES) has emerged as a promising technique in the field of bioengineering and biomedicine, particularly in bone regeneration and cell differentiation. ES using alternating current (AC) is based on the periodic reversal of current direction, which generates oscillating electric...

Descripción completa

Detalles Bibliográficos
Autores: Bocio-Núñez, Jesús, Montoya García, María José, Vázquez Gámez, María de los Ángeles, Martín Fernández, Daniel, Chacón, Pedro, Rico Corral, Miguel Ángel, Colmenero, Miguel Ángel, Yúfera García, Alberto, Giner García, Mercedes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:dnet:idus________::75a72b5406741efa085132ec49b3b844
Acceso en línea:https://hdl.handle.net/11441/185168
https://doi.org/10.1002/biof.70097Digital Object Identifier (DOI)
Access Level:acceso abierto
Palabra clave:Alternating current
Electric-stimulation
MC3T3-E1
Osteoblasts
Tissue engineering
id ES_e8eb4446ecc5c928a3fcdd05df8cc7ee
oai_identifier_str oai:dnet:idus________::75a72b5406741efa085132ec49b3b844
network_acronym_str ES
network_name_str España
repository_id_str
spelling Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts DifferentiationBocio-Núñez, JesúsMontoya García, María JoséVázquez Gámez, María de los ÁngelesMartín Fernández, DanielChacón, PedroRico Corral, Miguel ÁngelColmenero, Miguel ÁngelYúfera García, AlbertoGiner García, MercedesAlternating currentElectric-stimulationMC3T3-E1OsteoblastsTissue engineeringElectrical stimulation (ES) has emerged as a promising technique in the field of bioengineering and biomedicine, particularly in bone regeneration and cell differentiation. ES using alternating current (AC) is based on the periodic reversal of current direction, which generates oscillating electric fields. The application of an electric field has effects on cell growth and differentiation, as well as on morphology and migration. This study aimed to explore the effect of applying AC electrostimulation within the proliferation, differentiation, and morphology process of osteoblastic cells. The electrical stimulation signals were daily applied for 3 h during 14 days. Different frequencies were tested (1 Hz, 10 Hz, 100 Hz, and 1 kHz), with amplitudes of 125, 250, 500, 750, 1000, and 1500 mV/mm. Cell viability was estimated using the AlamarBlue, and MC3T3-E1 differentiation levels were evaluated through alkaline phosphatase (ALP) activity. RUNX2, OSX, ALP, OPG, and RANKL gene expression was assessed by RT-PCR. Morphological analysis was performed through cell transfection followed by immunofluorescence. Statistical analysis was conducted by SPSS.23 and graphs generated through Graph-pad. Viability and ALP activity were optimal at 10 Hz. Once the frequency was defined, RUNX2, OSX, ALP, OPG, and RANKL gene expression revealed an increase in the differentiation and osteogenic activity levels at 10 Hz and 500–750 mV/mm. As well as, morphological studies showed an increase in the area, pseudopodia length, and numbers at 500 mV 10 Hz conditions. The optimal ES condition to differentiate MC3T3-E1 cells is 10 Hz 500–750 mV/mm. Electrostimulation has emerged as a promising technique in the field of bioengineering and biomedicine, particularly in bone regeneration and cell early maturation.WileyCitología e Histología Normal y PatológicaMedicinaTecnología ElectrónicaCTS211: Metabolismo Cálcico, Hipertensión y ArteriosclerosisBIO132: CitoQuímica UltraestructuralTIC178: Diseño y Test de Circuitos Integrados de Señal MixtaJunta de Andalucía2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/185168https://doi.org/10.1002/biof.70097Digital Object Identifier (DOI)reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBiofactors, 52 (2), e70097. P18-FR-2038https://iubmb.onlinelibrary.wiley.com/doi/10.1002/biof.70097info:eu-repo/semantics/openAccessoai:dnet:idus________::75a72b5406741efa085132ec49b3b8442026-06-17T12:51:07Z
dc.title.none.fl_str_mv Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
title Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
spellingShingle Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
Bocio-Núñez, Jesús
Alternating current
Electric-stimulation
MC3T3-E1
Osteoblasts
Tissue engineering
title_short Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
title_full Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
title_fullStr Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
title_full_unstemmed Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
title_sort Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
dc.creator.none.fl_str_mv Bocio-Núñez, Jesús
Montoya García, María José
Vázquez Gámez, María de los Ángeles
Martín Fernández, Daniel
Chacón, Pedro
Rico Corral, Miguel Ángel
Colmenero, Miguel Ángel
Yúfera García, Alberto
Giner García, Mercedes
author Bocio-Núñez, Jesús
author_facet Bocio-Núñez, Jesús
Montoya García, María José
Vázquez Gámez, María de los Ángeles
Martín Fernández, Daniel
Chacón, Pedro
Rico Corral, Miguel Ángel
Colmenero, Miguel Ángel
Yúfera García, Alberto
Giner García, Mercedes
author_role author
author2 Montoya García, María José
Vázquez Gámez, María de los Ángeles
Martín Fernández, Daniel
Chacón, Pedro
Rico Corral, Miguel Ángel
Colmenero, Miguel Ángel
Yúfera García, Alberto
Giner García, Mercedes
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Citología e Histología Normal y Patológica
Medicina
Tecnología Electrónica
CTS211: Metabolismo Cálcico, Hipertensión y Arteriosclerosis
BIO132: CitoQuímica Ultraestructural
TIC178: Diseño y Test de Circuitos Integrados de Señal Mixta
Junta de Andalucía
dc.subject.none.fl_str_mv Alternating current
Electric-stimulation
MC3T3-E1
Osteoblasts
Tissue engineering
topic Alternating current
Electric-stimulation
MC3T3-E1
Osteoblasts
Tissue engineering
description Electrical stimulation (ES) has emerged as a promising technique in the field of bioengineering and biomedicine, particularly in bone regeneration and cell differentiation. ES using alternating current (AC) is based on the periodic reversal of current direction, which generates oscillating electric fields. The application of an electric field has effects on cell growth and differentiation, as well as on morphology and migration. This study aimed to explore the effect of applying AC electrostimulation within the proliferation, differentiation, and morphology process of osteoblastic cells. The electrical stimulation signals were daily applied for 3 h during 14 days. Different frequencies were tested (1 Hz, 10 Hz, 100 Hz, and 1 kHz), with amplitudes of 125, 250, 500, 750, 1000, and 1500 mV/mm. Cell viability was estimated using the AlamarBlue, and MC3T3-E1 differentiation levels were evaluated through alkaline phosphatase (ALP) activity. RUNX2, OSX, ALP, OPG, and RANKL gene expression was assessed by RT-PCR. Morphological analysis was performed through cell transfection followed by immunofluorescence. Statistical analysis was conducted by SPSS.23 and graphs generated through Graph-pad. Viability and ALP activity were optimal at 10 Hz. Once the frequency was defined, RUNX2, OSX, ALP, OPG, and RANKL gene expression revealed an increase in the differentiation and osteogenic activity levels at 10 Hz and 500–750 mV/mm. As well as, morphological studies showed an increase in the area, pseudopodia length, and numbers at 500 mV 10 Hz conditions. The optimal ES condition to differentiate MC3T3-E1 cells is 10 Hz 500–750 mV/mm. Electrostimulation has emerged as a promising technique in the field of bioengineering and biomedicine, particularly in bone regeneration and cell early maturation.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/185168
https://doi.org/10.1002/biof.70097Digital Object Identifier (DOI)
url https://hdl.handle.net/11441/185168
https://doi.org/10.1002/biof.70097Digital Object Identifier (DOI)
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Biofactors, 52 (2), e70097.
P18-FR-2038
https://iubmb.onlinelibrary.wiley.com/doi/10.1002/biof.70097
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422983204831232
score 15,812429

Similares en LA Referencia