Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets
13 Pág.
| Autores: | , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/331548 |
| Acceso en línea: | http://hdl.handle.net/10261/331548 https://api.elsevier.com/content/abstract/scopus_id/85085384435 |
| Access Level: | acceso abierto |
| Palabra clave: | C-type lectin CLEC12A Dendritic cells Monoclonal antibody Pig |
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Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell SubsetsÁlvarez, BelénNieto-Pelegrín, E.Martínez De la Riva, PalomaToki, D.Poderoso, TeresaRevilla Calvo, ConcepciónUenishi, H.Ezquerra Martínez, ÁngelDomínguez, JavierC-type lectinCLEC12ADendritic cellsMonoclonal antibodyPig13 Pág.CLEC12A has been proposed as a suitable target for delivering antigen to dendritic cells (DCs) to enhance vaccine efficacy both in human and mouse. In this study, we have characterized the porcine homolog of CLEC12A (poCLEC12A). Using new monoclonal antibodies (mAb), raised against its ectodomain, poCLEC12A was found to be expressed on alveolar macrophages, blood conventional type 1 and type 2 DCs and plasmacytoid DCs, but not on monocytes, T cells, B cells or NK cells, in contrast to its human and murine homologs. Western blot analysis showed that in alveolar macrophages this receptor is expressed both as a monomer and a dimer. After binding to DCs, anti- poCLEC12A mAb was efficiently internalized. No significant changes were observed in TNFα or IFNα secretion by plasmacytoid DCs stimulated with either CpGs (ODN2216) or polyinosinic-polycytidylic acid (poly I:C), upon incubation with mAb. These results provide the basis for future investigations aimed to assess the ability of anti-poCLEC12A mAbs to improve vaccine efficacy by targeting antigen to DCs.This work was supported by grant AGL2015-66187-P from Ministry of Economy, Industry and Competitiveness of Spain.Peer reviewedFrontiers MediaMinisterio de Economía, Industria y Competitividad (España)Nieto-Pelegrín, E. [0000-0003-2755-2641]Martínez De la Riva, Paloma [0000-0003-3097-022X]Poderoso, Teresa [0000-0002-1406-000X]Revilla Calvo, Concepción [0000-0002-8197-4186]Uenishi, H. [0000-0002-3899-9580]Ezquerra Martínez, Ángel [0000-0002-1824-5087]Domínguez, Javier [0000-0001-7256-388X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/331548https://api.elsevier.com/content/abstract/scopus_id/85085384435reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2015-66187-PDepartamento de Biotecnologíahttps://doi.org/10.3389/fimmu.2020.00863Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3315482026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets |
| title |
Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets |
| spellingShingle |
Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets Álvarez, Belén C-type lectin CLEC12A Dendritic cells Monoclonal antibody Pig |
| title_short |
Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets |
| title_full |
Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets |
| title_fullStr |
Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets |
| title_full_unstemmed |
Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets |
| title_sort |
Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets |
| dc.creator.none.fl_str_mv |
Álvarez, Belén Nieto-Pelegrín, E. Martínez De la Riva, Paloma Toki, D. Poderoso, Teresa Revilla Calvo, Concepción Uenishi, H. Ezquerra Martínez, Ángel Domínguez, Javier |
| author |
Álvarez, Belén |
| author_facet |
Álvarez, Belén Nieto-Pelegrín, E. Martínez De la Riva, Paloma Toki, D. Poderoso, Teresa Revilla Calvo, Concepción Uenishi, H. Ezquerra Martínez, Ángel Domínguez, Javier |
| author_role |
author |
| author2 |
Nieto-Pelegrín, E. Martínez De la Riva, Paloma Toki, D. Poderoso, Teresa Revilla Calvo, Concepción Uenishi, H. Ezquerra Martínez, Ángel Domínguez, Javier |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Economía, Industria y Competitividad (España) Nieto-Pelegrín, E. [0000-0003-2755-2641] Martínez De la Riva, Paloma [0000-0003-3097-022X] Poderoso, Teresa [0000-0002-1406-000X] Revilla Calvo, Concepción [0000-0002-8197-4186] Uenishi, H. [0000-0002-3899-9580] Ezquerra Martínez, Ángel [0000-0002-1824-5087] Domínguez, Javier [0000-0001-7256-388X] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
C-type lectin CLEC12A Dendritic cells Monoclonal antibody Pig |
| topic |
C-type lectin CLEC12A Dendritic cells Monoclonal antibody Pig |
| description |
13 Pág. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/331548 https://api.elsevier.com/content/abstract/scopus_id/85085384435 |
| url |
http://hdl.handle.net/10261/331548 https://api.elsevier.com/content/abstract/scopus_id/85085384435 |
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Inglés |
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Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2015-66187-P Departamento de Biotecnología https://doi.org/10.3389/fimmu.2020.00863 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Frontiers Media |
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Frontiers Media |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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