Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products

Producción Científica

Detalhes bibliográficos
Autores: Cantera Ruiz De Pellon, Sara, Lebrero Fernández, Raquel, Rodríguez, Suní, García Encina, Pedro Antonio, Muñoz Torre, Raúl
Tipo de documento: artigo
Data de publicação:2017
País:España
Recursos:Universidad de Valladolid
Repositório:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/24877
Acesso em linha:https://doi.org/10.1016/j.cej.2017.07.027
http://uvadoc.uva.es/handle/10324/24877
Access Level:Acceso aberto
Palavra-chave:Ordeño biológico
Metano
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spelling Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value productsCantera Ruiz De Pellon, SaraLebrero Fernández, RaquelRodríguez, SuníGarcía Encina, Pedro AntonioMuñoz Torre, RaúlOrdeño biológicoMetanoProducción CientíficaThis communication showed for the first time that the methanotrophic strain Methylomicrobium alcaliphilum 20Z (M. alcaliphilum 20Z) can efficiently synthesize and excrete (through a tailored bio-milking process) ectoine under continuous mode using methane (CH4) as the sole energy and carbon source. First, three consecutive 50 h fed batch fermentations consisting of alternating high salinity (6% NaCl for 24 h) and low salinity (0% NaCl for 24 h) cultivation stages were carried out in triplicate to determine the influence of sudden modifications in media salinity on ectoine synthesis and excretion. The results demonstrated that M. alcaliphilum 20Z exhibited a rapid response to osmotic shocks, which resulted in the release of the accumulated ectoine under hyposmotic shocks and the immediate uptake of the previously excreted ectoine during hyperosmotic shocks. A second experiment was carried out under continuous cultivation mode in two sequential stirred tank reactors operated at NaCl concentrations of 0 and 6%. Cells exhibited a constant intra-cellular ectoine concentration of 70.4 ± 14.3 mg g biomass−1 along the entire operation period when cultivated at a NaCl concentration of 6%. The centrifugation of the cultivation broth followed by a hyposmotic shock resulted in the excretion of ∼70% of the total intra-cellular ectoine. In brief, this research shows the feasibility of the continuous bioconversion of diluted CH4 emissions into high added-value products such as ectoine, which can turn greenhouse gas (GHG) abatement into a sustainable and profitable process.Ministerio de Economía, Industria y Competitividad (Project CTM2015-70442-R and Red NOVEDAR)Elsevier2017info:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1016/j.cej.2017.07.027http://uvadoc.uva.es/handle/10324/24877reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttp://www.sciencedirect.com/science/article/pii/S1385894717311749info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/248772026-06-13T12:44:47Z
dc.title.none.fl_str_mv Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products
title Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products
spellingShingle Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products
Cantera Ruiz De Pellon, Sara
Ordeño biológico
Metano
title_short Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products
title_full Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products
title_fullStr Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products
title_full_unstemmed Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products
title_sort Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products
dc.creator.none.fl_str_mv Cantera Ruiz De Pellon, Sara
Lebrero Fernández, Raquel
Rodríguez, Suní
García Encina, Pedro Antonio
Muñoz Torre, Raúl
author Cantera Ruiz De Pellon, Sara
author_facet Cantera Ruiz De Pellon, Sara
Lebrero Fernández, Raquel
Rodríguez, Suní
García Encina, Pedro Antonio
Muñoz Torre, Raúl
author_role author
author2 Lebrero Fernández, Raquel
Rodríguez, Suní
García Encina, Pedro Antonio
Muñoz Torre, Raúl
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ordeño biológico
Metano
topic Ordeño biológico
Metano
description Producción Científica
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.cej.2017.07.027
http://uvadoc.uva.es/handle/10324/24877
url https://doi.org/10.1016/j.cej.2017.07.027
http://uvadoc.uva.es/handle/10324/24877
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.sciencedirect.com/science/article/pii/S1385894717311749
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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