Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis

Background: using renewable feedstock sources for biodiesel production seems to be a promising strategy and even more so when enzymatic catalysis with lipases are used. However, it is well known that these enzymes could be inactivated by reaction conditions such as temperature or alcohol concentrati...

Descripción completa

Detalles Bibliográficos
Autores: Bonet-Ragel, Kírian|||0000-0002-0041-7493, Canet, Albert|||0009-0000-8487-8176, Benaiges, M. Dolors|||0000-0002-6840-1981, Valero, Francisco|||0000-0003-0429-9620
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:catalán
OAI Identifier:oai:ddd.uab.cat:200898
Acceso en línea:https://ddd.uab.cat/record/200898
https://dx.doi.org/urn:doi:10.1002/jctb.5399
Access Level:acceso abierto
Palabra clave:Rhizopus oryzae lipase
Biodiesel synthesis
Waste oil source
Water activity
Ethanolysis
Methanolysis
id ES_e896ff6d8065a2119df8095e080eeaf5
oai_identifier_str oai:ddd.uab.cat:200898
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesisBonet-Ragel, Kírian|||0000-0002-0041-7493Canet, Albert|||0009-0000-8487-8176Benaiges, M. Dolors|||0000-0002-6840-1981Valero, Francisco|||0000-0003-0429-9620Rhizopus oryzae lipaseBiodiesel synthesisWaste oil sourceWater activityEthanolysisMethanolysisBackground: using renewable feedstock sources for biodiesel production seems to be a promising strategy and even more so when enzymatic catalysis with lipases are used. However, it is well known that these enzymes could be inactivated by reaction conditions such as temperature or alcohol concentration. In this work, the effect of temperature and initial water activity (aw) value on immobilised recombinant Rhizopus oryzae lipase (rROL) were studied. Methanolysis and ethanolysis reactions using alperujo oil with three different stepwise addition strategies were employed. - Results: recombinant 1,3-positional selective rROL covalently immobilised on polymethacrylate amino-epoxy activated support showed maximum initial reaction rate at low aw value (0.093). It was found that 30 °C was the optimal temperature in terms of biocatalyst stability during transesterification reactions. Adding alcohol at once, ethanol was clearly a better acyl-acceptor in terms of stability than methanol. Productivity was found to be 2-fold higher when five pulses of ethanol were used instead of methanol. - Conclusions: alperujo oil has great potential as a low cost feedstock for biodiesel production through enzymatic catalysis using a nearly semi-continuous alcohol addition strategy. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/200898https://dx.doi.org/urn:doi:10.1002/jctb.5399reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaCataláncatMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2013/42391-RAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-452open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2008982026-06-06T12:50:31Z
dc.title.none.fl_str_mv Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
title Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
spellingShingle Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
Bonet-Ragel, Kírian|||0000-0002-0041-7493
Rhizopus oryzae lipase
Biodiesel synthesis
Waste oil source
Water activity
Ethanolysis
Methanolysis
title_short Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
title_full Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
title_fullStr Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
title_full_unstemmed Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
title_sort Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
dc.creator.none.fl_str_mv Bonet-Ragel, Kírian|||0000-0002-0041-7493
Canet, Albert|||0009-0000-8487-8176
Benaiges, M. Dolors|||0000-0002-6840-1981
Valero, Francisco|||0000-0003-0429-9620
author Bonet-Ragel, Kírian|||0000-0002-0041-7493
author_facet Bonet-Ragel, Kírian|||0000-0002-0041-7493
Canet, Albert|||0009-0000-8487-8176
Benaiges, M. Dolors|||0000-0002-6840-1981
Valero, Francisco|||0000-0003-0429-9620
author_role author
author2 Canet, Albert|||0009-0000-8487-8176
Benaiges, M. Dolors|||0000-0002-6840-1981
Valero, Francisco|||0000-0003-0429-9620
author2_role author
author
author
dc.subject.none.fl_str_mv Rhizopus oryzae lipase
Biodiesel synthesis
Waste oil source
Water activity
Ethanolysis
Methanolysis
topic Rhizopus oryzae lipase
Biodiesel synthesis
Waste oil source
Water activity
Ethanolysis
Methanolysis
description Background: using renewable feedstock sources for biodiesel production seems to be a promising strategy and even more so when enzymatic catalysis with lipases are used. However, it is well known that these enzymes could be inactivated by reaction conditions such as temperature or alcohol concentration. In this work, the effect of temperature and initial water activity (aw) value on immobilised recombinant Rhizopus oryzae lipase (rROL) were studied. Methanolysis and ethanolysis reactions using alperujo oil with three different stepwise addition strategies were employed. - Results: recombinant 1,3-positional selective rROL covalently immobilised on polymethacrylate amino-epoxy activated support showed maximum initial reaction rate at low aw value (0.093). It was found that 30 °C was the optimal temperature in terms of biocatalyst stability during transesterification reactions. Adding alcohol at once, ethanol was clearly a better acyl-acceptor in terms of stability than methanol. Productivity was found to be 2-fold higher when five pulses of ethanol were used instead of methanol. - Conclusions: alperujo oil has great potential as a low cost feedstock for biodiesel production through enzymatic catalysis using a nearly semi-continuous alcohol addition strategy.
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/200898
https://dx.doi.org/urn:doi:10.1002/jctb.5399
url https://ddd.uab.cat/record/200898
https://dx.doi.org/urn:doi:10.1002/jctb.5399
dc.language.none.fl_str_mv Catalán
cat
language_invalid_str_mv Catalán
language cat
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2013/42391-R
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-452
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422955372478464
score 15,300719

Similares en LA Referencia