Role of Mitochondrial Complex IV in Age-Dependent Obesity.
Aging is associated with progressive white adipose tissue (WAT) enlargement initiated early in life, but the molecular mechanisms involved remain unknown. Here we show that mitochondrial complex IV (CIV) activity and assembly are already repressed in white adipocytes of middle-aged mice and involve...
| Autores: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/114790 |
| Acceso en línea: | https://hdl.handle.net/2445/114790 |
| Access Level: | acceso abierto |
| Palabra clave: | Envelliment Obesitat ADN mitocondrial Aging Obesity Mitochondrial DNA |
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Role of Mitochondrial Complex IV in Age-Dependent Obesity.Soro-Arnaiz, InesYang Li, Qilong OscarTorres-Capelli, MarMeléndez-Rodríguez, FlorindaVeiga, Sonia Rosa Pereira daVeys, KoenSebastián Muñoz, DavidElorza, AinaraTello Velasco, DanielHernansanz Agustín, PabloCogliati, SaraMoreno-Navarrete, José MaríaBalsa, EduardoFuertes, EstherRomanos, EduardoMartínez Ruiz, AntonioEnriquez, Jose AntonioFernández-Real Lemos, José ManuelZorzano Olarte, AntonioDe Bock, KatrienAragonés, JuliánEnvellimentObesitatADN mitocondrialAgingObesityMitochondrial DNAAging is associated with progressive white adipose tissue (WAT) enlargement initiated early in life, but the molecular mechanisms involved remain unknown. Here we show that mitochondrial complex IV (CIV) activity and assembly are already repressed in white adipocytes of middle-aged mice and involve a HIF1A-dependent decline of essential CIV components such as COX5B. At the molecular level, HIF1A binds to the Cox5b proximal promoter and represses its expression. Silencing of Cox5b decreased fatty acid oxidation and promoted intracellular lipid accumulation. Moreover, local in vivo Cox5b silencing in WAT of young mice increased the size of adipocytes, whereas restoration of COX5B expression in aging mice counteracted adipocyte enlargement. An age-dependent reduction in COX5B gene expression was also found in human visceral adipose tissue. Collectively, our findings establish a pivotal role for CIV dysfunction in progressive white adipocyte enlargement during aging, which can be restored to alleviate age-dependent WAT expansion.Elsevier2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/114790Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.celrep.2016.08.041Cell Reports, 2016https://doi.org/10.1016/j.celrep.2016.08.041info:eu-repo/grantAgreement/EC/FP7/282551cc-by (c) Soro-Arnaiz, Ines et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1147902026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Role of Mitochondrial Complex IV in Age-Dependent Obesity. |
| title |
Role of Mitochondrial Complex IV in Age-Dependent Obesity. |
| spellingShingle |
Role of Mitochondrial Complex IV in Age-Dependent Obesity. Soro-Arnaiz, Ines Envelliment Obesitat ADN mitocondrial Aging Obesity Mitochondrial DNA |
| title_short |
Role of Mitochondrial Complex IV in Age-Dependent Obesity. |
| title_full |
Role of Mitochondrial Complex IV in Age-Dependent Obesity. |
| title_fullStr |
Role of Mitochondrial Complex IV in Age-Dependent Obesity. |
| title_full_unstemmed |
Role of Mitochondrial Complex IV in Age-Dependent Obesity. |
| title_sort |
Role of Mitochondrial Complex IV in Age-Dependent Obesity. |
| dc.creator.none.fl_str_mv |
Soro-Arnaiz, Ines Yang Li, Qilong Oscar Torres-Capelli, Mar Meléndez-Rodríguez, Florinda Veiga, Sonia Rosa Pereira da Veys, Koen Sebastián Muñoz, David Elorza, Ainara Tello Velasco, Daniel Hernansanz Agustín, Pablo Cogliati, Sara Moreno-Navarrete, José María Balsa, Eduardo Fuertes, Esther Romanos, Eduardo Martínez Ruiz, Antonio Enriquez, Jose Antonio Fernández-Real Lemos, José Manuel Zorzano Olarte, Antonio De Bock, Katrien Aragonés, Julián |
| author |
Soro-Arnaiz, Ines |
| author_facet |
Soro-Arnaiz, Ines Yang Li, Qilong Oscar Torres-Capelli, Mar Meléndez-Rodríguez, Florinda Veiga, Sonia Rosa Pereira da Veys, Koen Sebastián Muñoz, David Elorza, Ainara Tello Velasco, Daniel Hernansanz Agustín, Pablo Cogliati, Sara Moreno-Navarrete, José María Balsa, Eduardo Fuertes, Esther Romanos, Eduardo Martínez Ruiz, Antonio Enriquez, Jose Antonio Fernández-Real Lemos, José Manuel Zorzano Olarte, Antonio De Bock, Katrien Aragonés, Julián |
| author_role |
author |
| author2 |
Yang Li, Qilong Oscar Torres-Capelli, Mar Meléndez-Rodríguez, Florinda Veiga, Sonia Rosa Pereira da Veys, Koen Sebastián Muñoz, David Elorza, Ainara Tello Velasco, Daniel Hernansanz Agustín, Pablo Cogliati, Sara Moreno-Navarrete, José María Balsa, Eduardo Fuertes, Esther Romanos, Eduardo Martínez Ruiz, Antonio Enriquez, Jose Antonio Fernández-Real Lemos, José Manuel Zorzano Olarte, Antonio De Bock, Katrien Aragonés, Julián |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Envelliment Obesitat ADN mitocondrial Aging Obesity Mitochondrial DNA |
| topic |
Envelliment Obesitat ADN mitocondrial Aging Obesity Mitochondrial DNA |
| description |
Aging is associated with progressive white adipose tissue (WAT) enlargement initiated early in life, but the molecular mechanisms involved remain unknown. Here we show that mitochondrial complex IV (CIV) activity and assembly are already repressed in white adipocytes of middle-aged mice and involve a HIF1A-dependent decline of essential CIV components such as COX5B. At the molecular level, HIF1A binds to the Cox5b proximal promoter and represses its expression. Silencing of Cox5b decreased fatty acid oxidation and promoted intracellular lipid accumulation. Moreover, local in vivo Cox5b silencing in WAT of young mice increased the size of adipocytes, whereas restoration of COX5B expression in aging mice counteracted adipocyte enlargement. An age-dependent reduction in COX5B gene expression was also found in human visceral adipose tissue. Collectively, our findings establish a pivotal role for CIV dysfunction in progressive white adipocyte enlargement during aging, which can be restored to alleviate age-dependent WAT expansion. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/114790 |
| url |
https://hdl.handle.net/2445/114790 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1016/j.celrep.2016.08.041 Cell Reports, 2016 https://doi.org/10.1016/j.celrep.2016.08.041 info:eu-repo/grantAgreement/EC/FP7/282551 |
| dc.rights.none.fl_str_mv |
cc-by (c) Soro-Arnaiz, Ines et al., 2016 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) Soro-Arnaiz, Ines et al., 2016 http://creativecommons.org/licenses/by/3.0/es |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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Articles publicats en revistes (Bioquímica i Biomedicina Molecular) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
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Universidad de Barcelona |
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Dipòsit Digital de la UB |
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Dipòsit Digital de la UB |
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1869422951652130816 |
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15,300719 |