Control of growth and pattering : a novel rol of JAK/STAT in regulating morphogen production and signalling = Control del crecimiento y patrón : un nuevo rol de JAK/STAT regulando la producción y señalización de morfógenos
During my thesis I studied the developmental roles of the JAK/STAT pathway in the wing imaginal disc and we show that it controls organ size and fate specification by regulating morphogen production and activity. We show three distinguishable biological functions of JAK/STAT depending on the territo...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/402782 |
| Acceso en línea: | http://hdl.handle.net/10803/402782 |
| Access Level: | acceso abierto |
| Palabra clave: | Factors de creixement Factores de crecimiento Growth factors Drosòfila Drosophila Transducció de senyal cel·lular Transducción de la señal celular Cellular signal transduction Morfogènesi Morfogénesis Morphogenesis Ciències Experimentals i Matemàtiques 575 |
| Sumario: | During my thesis I studied the developmental roles of the JAK/STAT pathway in the wing imaginal disc and we show that it controls organ size and fate specification by regulating morphogen production and activity. We show three distinguishable biological functions of JAK/STAT depending on the territory and developmental stage. Briefly, JAK/STAT is required in Drosophila limb development to facilitate the activities of Wingless, Hedgehog and the Dpp morphogens in exerting their fate- and growth-promoting functions. Early in development, JAK/STAT is required to guarantee Wg-mediated appendage specification by repressing EGFR target gene expression and thus restricts the notum fate to the most proximal territories of the wing primordium. Later in development, once the wing has been specified, JAK/STAT regulates organ size by ensuring the stable and localized expression of the Dpp organizer at the center of the wing primordium. This function relies on an autonomous and compartment-specific requirement of JAK/STAT in maintaining the size of the posterior territory. We show that JAK/STAT promotes the cycling and survival of P cells and identified diap1 and CycA as the two critical genes regulated by JAK/STAT and contributing to the growth of the Hh expressing cell population. The specific requirement of the P compartment for JAK/STAT serves to counteract the negative effect of Engrailed on cell cycling and survival. Thus, we identified a mechanism by which pro-survival cues and mitotic cyclins ensure the maintenance of a stable pool of morphogen-producing cells. Finally, we also show that JAK/STAT and its growth-promoting activity in the hinge region function as a topological barrier that isolates the body wall and appendage sources of Dpp to delimit its organizing activity to the developing appendage. |
|---|