Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?

The use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnosti...

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Autores: González Fernández, Cristina, Gómez Pastora, Jenifer, Basauri Penagos, Arantza, Fallanza Torices, Marcos|||0000-0003-3834-5787, Bringas Elizalde, Eugenio|||0000-0001-8197-6547, Chalmers, Jeffrey J., Ortiz Uribe, Inmaculada|||0000-0002-3257-4821
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/18652
Acceso en línea:http://hdl.handle.net/10902/18652
Access Level:acceso abierto
Palabra clave:Particle magnetophoresis
CFD
Cross section
Chip fabrication
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spelling Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?González Fernández, CristinaGómez Pastora, JeniferBasauri Penagos, ArantzaFallanza Torices, Marcos|||0000-0003-3834-5787Bringas Elizalde, Eugenio|||0000-0001-8197-6547Chalmers, Jeffrey J.Ortiz Uribe, Inmaculada|||0000-0002-3257-4821Particle magnetophoresisCFDCross sectionChip fabricationThe use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnostic tests. Particle recovery with permanent magnets in continuous-flow microdevices has gathered great attention in the last decade due to the multiple advantages of microfluidics. As such, great efforts have been made to determine the magnetic and fluidic conditions for achieving complete particle capture; however, less attention has been paid to the effect of the channel geometry on the system performance, although it is key for designing systems that simultaneously provide high particle recovery and flow rates. Herein, we address the optimization of Y-Y-shaped microchannels, where magnetic beads are separated from blood and collected into a buffer stream by applying an external magnetic field. The influence of several geometrical features (namely cross section shape, thickness, length, and volume) on both bead recovery and system throughput is studied. For that purpose, we employ an experimentally validated Computational Fluid Dynamics (CFD) numerical model that considers the dominant forces acting on the beads during separation. Our results indicate that rectangular, long devices display the best performance as they deliver high particle recovery and high throughput. Thus, this methodology could be applied to the rational design of lab-on-a-chip devices for any magnetically driven purification, enrichment or isolation.This research was funded by the Spanish Ministry of Science, Innovation and Universities under the project RTI2018-093310-B-I00, and the FPU and FPI postgraduate research grants (FPU18/03525; BES-2016-077206). Financial support from the National Heart, Lung, and Blood Institute from the United States National Institutes of Health (1R01HL131720-01A1) has also been receivedMDPIUniversidad de Cantabria20202020-05-27journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/18652Sensors, 2020, 20(11), 3030reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/186522026-06-02T12:39:31Z
dc.title.none.fl_str_mv Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?
title Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?
spellingShingle Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?
González Fernández, Cristina
Particle magnetophoresis
CFD
Cross section
Chip fabrication
title_short Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?
title_full Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?
title_fullStr Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?
title_full_unstemmed Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?
title_sort Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?
dc.creator.none.fl_str_mv González Fernández, Cristina
Gómez Pastora, Jenifer
Basauri Penagos, Arantza
Fallanza Torices, Marcos|||0000-0003-3834-5787
Bringas Elizalde, Eugenio|||0000-0001-8197-6547
Chalmers, Jeffrey J.
Ortiz Uribe, Inmaculada|||0000-0002-3257-4821
author González Fernández, Cristina
author_facet González Fernández, Cristina
Gómez Pastora, Jenifer
Basauri Penagos, Arantza
Fallanza Torices, Marcos|||0000-0003-3834-5787
Bringas Elizalde, Eugenio|||0000-0001-8197-6547
Chalmers, Jeffrey J.
Ortiz Uribe, Inmaculada|||0000-0002-3257-4821
author_role author
author2 Gómez Pastora, Jenifer
Basauri Penagos, Arantza
Fallanza Torices, Marcos|||0000-0003-3834-5787
Bringas Elizalde, Eugenio|||0000-0001-8197-6547
Chalmers, Jeffrey J.
Ortiz Uribe, Inmaculada|||0000-0002-3257-4821
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
dc.subject.none.fl_str_mv Particle magnetophoresis
CFD
Cross section
Chip fabrication
topic Particle magnetophoresis
CFD
Cross section
Chip fabrication
description The use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnostic tests. Particle recovery with permanent magnets in continuous-flow microdevices has gathered great attention in the last decade due to the multiple advantages of microfluidics. As such, great efforts have been made to determine the magnetic and fluidic conditions for achieving complete particle capture; however, less attention has been paid to the effect of the channel geometry on the system performance, although it is key for designing systems that simultaneously provide high particle recovery and flow rates. Herein, we address the optimization of Y-Y-shaped microchannels, where magnetic beads are separated from blood and collected into a buffer stream by applying an external magnetic field. The influence of several geometrical features (namely cross section shape, thickness, length, and volume) on both bead recovery and system throughput is studied. For that purpose, we employ an experimentally validated Computational Fluid Dynamics (CFD) numerical model that considers the dominant forces acting on the beads during separation. Our results indicate that rectangular, long devices display the best performance as they deliver high particle recovery and high throughput. Thus, this methodology could be applied to the rational design of lab-on-a-chip devices for any magnetically driven purification, enrichment or isolation.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-05-27
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10902/18652
url http://hdl.handle.net/10902/18652
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Sensors, 2020, 20(11), 3030
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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