Conjugation inhibitors effectively prevent plasmid transmission in natural environments

© 2021 Palencia-Gándara et al.

Detalles Bibliográficos
Autores: Palencia-Gándara, Carolina, Getino, María, Moyano, Gabriel, Redondo, Santiago, Fernández López, Raúl, Gonzalez-Zorn, B., Cruz, Fernando de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/255893
Acceso en línea:http://hdl.handle.net/10261/255893
Access Level:acceso abierto
Palabra clave:Escherichia coli
Bacterial conjugation
Antibiotic resistance
Conjugation inhibitor
Microcosm
Gut
Zebrafish
Mouse
Conjugation
Plasmids
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spelling Conjugation inhibitors effectively prevent plasmid transmission in natural environmentsPalencia-Gándara, CarolinaGetino, MaríaMoyano, GabrielRedondo, SantiagoFernández López, RaúlGonzalez-Zorn, B.Cruz, Fernando de laEscherichia coliBacterial conjugationAntibiotic resistanceConjugation inhibitorMicrocosmGutZebrafishMouseConjugationPlasmids© 2021 Palencia-Gándara et al.Plasmid conjugation is a major route for the spread of antibiotic resistance genes. Inhibiting conjugation has been proposed as a feasible strategy to stop or delay the propagation of antibiotic resistance genes. Several compounds have been shown to be conjugation inhibitors in vitro, specifically targeting the plasmid horizontal transfer machinery. However, the in vivo efficiency and the applicability of these compounds to clinical and environmental settings remained untested. Here we show that the synthetic fatty acid 2-hexadecynoic acid (2-HDA), when used as a fish food supplement, lowers the conjugation frequency of model plasmids up to 10-fold in controlled water microcosms. When added to the food for mice, 2-HDA diminished the conjugation efficiency 50-fold in controlled plasmid transfer assays carried out in the mouse gut. These results demonstrate the in vivo efficiency of conjugation inhibitors, paving the way for their potential application in clinical and environmental settings.The work performed by the de la Cruz research group was supported by the European Union Seventh Framework Program (FP7-HEALTH-2011-single-stage) “Evolution and Transfer of Antibiotic Resistance” (EvoTAR), grant agreement number 282004. The work performed by C.P.-G. and M.G. was supported by Ph.D. fellowships funded by the University of Cantabria. The work performed by the B.G.-Z. laboratory was supported by The EFFORT project (www.effort-against-amr.eu) FP7-KBBE-2013-7, grant agreement 613754.American Society for MicrobiologyEuropean CommissionUniversidad de CantabriaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/255893reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/282004info:eu-repo/grantAgreement/EC/FP7/613754http://dx.doi.org/10.1128/mBio.01277-21Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2558932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Conjugation inhibitors effectively prevent plasmid transmission in natural environments
title Conjugation inhibitors effectively prevent plasmid transmission in natural environments
spellingShingle Conjugation inhibitors effectively prevent plasmid transmission in natural environments
Palencia-Gándara, Carolina
Escherichia coli
Bacterial conjugation
Antibiotic resistance
Conjugation inhibitor
Microcosm
Gut
Zebrafish
Mouse
Conjugation
Plasmids
title_short Conjugation inhibitors effectively prevent plasmid transmission in natural environments
title_full Conjugation inhibitors effectively prevent plasmid transmission in natural environments
title_fullStr Conjugation inhibitors effectively prevent plasmid transmission in natural environments
title_full_unstemmed Conjugation inhibitors effectively prevent plasmid transmission in natural environments
title_sort Conjugation inhibitors effectively prevent plasmid transmission in natural environments
dc.creator.none.fl_str_mv Palencia-Gándara, Carolina
Getino, María
Moyano, Gabriel
Redondo, Santiago
Fernández López, Raúl
Gonzalez-Zorn, B.
Cruz, Fernando de la
author Palencia-Gándara, Carolina
author_facet Palencia-Gándara, Carolina
Getino, María
Moyano, Gabriel
Redondo, Santiago
Fernández López, Raúl
Gonzalez-Zorn, B.
Cruz, Fernando de la
author_role author
author2 Getino, María
Moyano, Gabriel
Redondo, Santiago
Fernández López, Raúl
Gonzalez-Zorn, B.
Cruz, Fernando de la
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Universidad de Cantabria
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Escherichia coli
Bacterial conjugation
Antibiotic resistance
Conjugation inhibitor
Microcosm
Gut
Zebrafish
Mouse
Conjugation
Plasmids
topic Escherichia coli
Bacterial conjugation
Antibiotic resistance
Conjugation inhibitor
Microcosm
Gut
Zebrafish
Mouse
Conjugation
Plasmids
description © 2021 Palencia-Gándara et al.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/255893
url http://hdl.handle.net/10261/255893
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/282004
info:eu-repo/grantAgreement/EC/FP7/613754
http://dx.doi.org/10.1128/mBio.01277-21
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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