Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation

Gold nanorods (AuNRs) have attracted attention in the field of biomedicine, particularly for their potential as photothermal agents capable of killing tumor cells by photothermic ablation. In this study, the synthesis of novel AuNRs stabilized with thiolated pectin (AuNR@SH-PEC) is reported. To achi...

ver descrição completa

Detalhes bibliográficos
Autores: Beltran, O., Luna, M., Gastelum, M., Costa-Santos, A., Cambón, A., Taboada Antelo, Pablo, López-Mata, M.A., Topete, A., Juarez, J.
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/21789
Acesso em linha:https://portalcientifico.sergas.gal//documentos/657f1ac93ea324404509bf88
http://hdl.handle.net/20.500.11940/21789
Access Level:acceso abierto
Palavra-chave:IDIS
id ES_e85f19d1fc33cd457bb884138bd8d09b
oai_identifier_str oai:runa.sergas.gal:20.500.11940/21789
network_acronym_str ES
network_name_str España
repository_id_str
spelling Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal AblationBeltran, O.Luna, M.Gastelum, M.Costa-Santos, A.Cambón, A.Taboada Antelo, PabloLópez-Mata, M.A.Topete, A.Juarez, J.IDISGold nanorods (AuNRs) have attracted attention in the field of biomedicine, particularly for their potential as photothermal agents capable of killing tumor cells by photothermic ablation. In this study, the synthesis of novel AuNRs stabilized with thiolated pectin (AuNR@SH-PEC) is reported. To achieve this, thiolated pectin (SH-PEC) was obtained by chemically binding cysteamine motifs to the pectin backbone. The success of the reaction was ascertained using FTIR-ATR. Subsequently, the SH-PEC was used to coat and stabilize the surface of AuNRs (AuNR@SH-PEC). In this context, different concentrations of SH-PEC (0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 mg/mL) were added to 0.50 mL of AuNRs suspended in CTAB, aiming to determine the experimental conditions under which AuNR@SH-PEC maintains stability. The results show that SH-PEC effectively replaced the CTAB adsorbed on the surface of AuNRs, enhancing the stability of AuNRs without affecting their optical properties. Additionally, scanning electron and atomic force microscopy confirmed that SH-PEC is adsorbed into the surface of the AuNRs. Importantly, the dimension size (60 × 15 nm) and the aspect ratio (4:1) remained consistent with those of AuNRs stabilized with CTAB. Then, the photothermal properties of gold nanorods were evaluated by irradiating the aqueous suspension of AuNR@SH-PEC with a CW laser (808 nm, 1 W). These results showed that photothermal conversion efficiency is similar to the photothermal conversion observed for AuNR-CTAB. Lastly, the cell viability assays confirmed that the SH-PEC coating enhanced the biocompatibility of AuNR@SH-PEC. Most important, the viability cell assays subjected to laser irradiation in the presence of AuNR@SH-PEC showed a decrease in the cell viability relative to the non-irradiated cells. These results suggest that AuNRs stabilized with thiolated pectin can potentially be exploited in the implementation of photothermal therapy.To CONAHCYT for the scholarships given to the Ph.D. students (Osvaldo Beltran, Mariangel Luna, Marisol Gastelum). To Universidad de Sonora and Universidad de Santiago de Compostela to facilitate their infrastructure.2023info:eu-repo/semantics/articlehttps://portalcientifico.sergas.gal//documentos/657f1ac93ea324404509bf88http://hdl.handle.net/20.500.11940/21789reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Ingléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/217892026-06-12T08:40:47Z
dc.title.none.fl_str_mv Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation
title Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation
spellingShingle Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation
Beltran, O.
IDIS
title_short Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation
title_full Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation
title_fullStr Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation
title_full_unstemmed Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation
title_sort Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation
dc.creator.none.fl_str_mv Beltran, O.
Luna, M.
Gastelum, M.
Costa-Santos, A.
Cambón, A.
Taboada Antelo, Pablo
López-Mata, M.A.
Topete, A.
Juarez, J.
author Beltran, O.
author_facet Beltran, O.
Luna, M.
Gastelum, M.
Costa-Santos, A.
Cambón, A.
Taboada Antelo, Pablo
López-Mata, M.A.
Topete, A.
Juarez, J.
author_role author
author2 Luna, M.
Gastelum, M.
Costa-Santos, A.
Cambón, A.
Taboada Antelo, Pablo
López-Mata, M.A.
Topete, A.
Juarez, J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv IDIS
topic IDIS
description Gold nanorods (AuNRs) have attracted attention in the field of biomedicine, particularly for their potential as photothermal agents capable of killing tumor cells by photothermic ablation. In this study, the synthesis of novel AuNRs stabilized with thiolated pectin (AuNR@SH-PEC) is reported. To achieve this, thiolated pectin (SH-PEC) was obtained by chemically binding cysteamine motifs to the pectin backbone. The success of the reaction was ascertained using FTIR-ATR. Subsequently, the SH-PEC was used to coat and stabilize the surface of AuNRs (AuNR@SH-PEC). In this context, different concentrations of SH-PEC (0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 mg/mL) were added to 0.50 mL of AuNRs suspended in CTAB, aiming to determine the experimental conditions under which AuNR@SH-PEC maintains stability. The results show that SH-PEC effectively replaced the CTAB adsorbed on the surface of AuNRs, enhancing the stability of AuNRs without affecting their optical properties. Additionally, scanning electron and atomic force microscopy confirmed that SH-PEC is adsorbed into the surface of the AuNRs. Importantly, the dimension size (60 × 15 nm) and the aspect ratio (4:1) remained consistent with those of AuNRs stabilized with CTAB. Then, the photothermal properties of gold nanorods were evaluated by irradiating the aqueous suspension of AuNR@SH-PEC with a CW laser (808 nm, 1 W). These results showed that photothermal conversion efficiency is similar to the photothermal conversion observed for AuNR-CTAB. Lastly, the cell viability assays confirmed that the SH-PEC coating enhanced the biocompatibility of AuNR@SH-PEC. Most important, the viability cell assays subjected to laser irradiation in the presence of AuNR@SH-PEC showed a decrease in the cell viability relative to the non-irradiated cells. These results suggest that AuNRs stabilized with thiolated pectin can potentially be exploited in the implementation of photothermal therapy.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://portalcientifico.sergas.gal//documentos/657f1ac93ea324404509bf88
http://hdl.handle.net/20.500.11940/21789
url https://portalcientifico.sergas.gal//documentos/657f1ac93ea324404509bf88
http://hdl.handle.net/20.500.11940/21789
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas
instname:Servizo Galego de Saúde (SERGAS)
instname_str Servizo Galego de Saúde (SERGAS)
reponame_str RUNA. Repositorio da Consellería de Sanidade e Sergas
collection RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422936138448896
score 15.812429

Registos relacionados