Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide

Beyond Xylella, Integrated Management Strategies for Mitigating Xylella fastidiosa Impact in Europe (BeXyl) (Grant Agreement 101060593). Partner/Coordinador principal: Blanca B. Landa del Castillo, Investigadora Científica del Instituto de Agricultura Sostenible (IAS-CSIC).

Detalles Bibliográficos
Autores: Baró, Aina, Saldarelli, Pasquale, Saponari, Maria, Montesinos, Emilio, Montesinos, Laura
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/310019
Acceso en línea:http://hdl.handle.net/10261/310019
https://api.elsevier.com/content/abstract/scopus_id/85144056391
Access Level:acceso abierto
Palabra clave:Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
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spelling Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptideBaró, AinaSaldarelli, PasqualeSaponari, MariaMontesinos, EmilioMontesinos, LauraPlant infectionsXylella fastidiosaBactericidalBifunctional peptideNicotiana benthamianaPlant defense elicitorBeyond Xylella, Integrated Management Strategies for Mitigating Xylella fastidiosa Impact in Europe (BeXyl) (Grant Agreement 101060593). Partner/Coordinador principal: Blanca B. Landa del Castillo, Investigadora Científica del Instituto de Agricultura Sostenible (IAS-CSIC).Transient expression of genes encoding peptides BP134 and BP178 by means of a Potato virus X (PVX) based-vector system, and treatment with synthetic peptides by endotherapy, were evaluated in the control of Xylella fastidiosa infections, in the model plant Nicotiana benthamiana. Transient production of BP178 significantly decreased disease severity compared to PVX and non-treated control (NTC) plants, without adverse effects. Plants treated with synthetic BP134 and BP178 showed consistently lower levels of disease than NTC plants. However, the coinfection with PVX-BP134 and X. fastidiosa caused detrimental effects resulting in plant death. The levels of X. fastidiosa in three zones sampled, upwards and downwards of the inoculation/treatment point, significantly decreased compared to the NTC plants, after the treatment with BP178, but not when BP178 was produced transiently. The effect of treatment and transient production of BP178 in the induction of defense-related genes was also studied. Synthetic BP178 applied by endotherapy induced the expression of ERF1, PR1a, PAL, PALII and WRKY25, while the transient expression of BP178 overexpressed the Cath, Cyc, PR4a, 9-LOX and Endochitinase B genes. Both treatments upregulated the expression of PR1, PR3, PR4 and CycT9299 genes compared to the NTC or PVX plants. It was concluded that the effect of BP178, either by endotherapy or by transient expression, on the control of the X. fastidiosa infections in N. benthamiana, was due in part to the induction of the plant defense system in addition to its bactericidal activity reported in previous studies. However, the protection observed when BP178 was transiently produced seems mainly mediated by the induction of plant defense, because the levels of X. fastidiosa were not significantly affected.This work was supported by grants from the European Union XF-ACTORS (Ref. 727987) and BeXyL (Ref. 101060593) projects. AB was recipient of a research grant from the Secretaria d’Universitats i Recerca, Departament d’Economia i Coneixement, Generalitat de Catalunya (Ref. 2018 FI B00334).Peer reviewedFrontiers MediaEuropean CommissionGeneralitat de Catalunya202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/310019https://api.elsevier.com/content/abstract/scopus_id/85144056391reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/HE/101060593info:eu-repo/grantAgreement/EC/H2020/727987https://doi.org/10.3389/fpls.2022.1061463Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3100192026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
spellingShingle Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
Baró, Aina
Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
title_short Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_full Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_fullStr Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_full_unstemmed Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_sort Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
dc.creator.none.fl_str_mv Baró, Aina
Saldarelli, Pasquale
Saponari, Maria
Montesinos, Emilio
Montesinos, Laura
author Baró, Aina
author_facet Baró, Aina
Saldarelli, Pasquale
Saponari, Maria
Montesinos, Emilio
Montesinos, Laura
author_role author
author2 Saldarelli, Pasquale
Saponari, Maria
Montesinos, Emilio
Montesinos, Laura
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Generalitat de Catalunya
dc.subject.none.fl_str_mv Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
topic Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
description Beyond Xylella, Integrated Management Strategies for Mitigating Xylella fastidiosa Impact in Europe (BeXyl) (Grant Agreement 101060593). Partner/Coordinador principal: Blanca B. Landa del Castillo, Investigadora Científica del Instituto de Agricultura Sostenible (IAS-CSIC).
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/310019
https://api.elsevier.com/content/abstract/scopus_id/85144056391
url http://hdl.handle.net/10261/310019
https://api.elsevier.com/content/abstract/scopus_id/85144056391
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/HE/101060593
info:eu-repo/grantAgreement/EC/H2020/727987
https://doi.org/10.3389/fpls.2022.1061463
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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