Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny

[EN] Pollen development is a crucial step in higher plants, which not only makes possible plant fertilization and seed formation, but also determines fruit quality and yield in crop species. Here, we reported a tomato T-DNA mutant, pollen deficient1 (pod1), characterized by an abnormal anther develo...

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Autores: Pérez Martín, F., Juan Yuste-Lisbona, Fernando, Pineda, B., García Sogo, Begoña, Del Olmo, I., Alché, Juan de Dios, Egea, Isabel, Flores, F.B., Piñeiro, M., Jarillo,Jose A., Angosto, T., Capel, J., Lozano, R., Moreno Ferrero, Vicente|||0000-0002-0345-7300
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/147423
Acceso en línea:https://riunet.upv.es/handle/10251/147423
Access Level:acceso abierto
Palabra clave:Tomato
Pollen development
Tapetum degradation
Mediator complex
T-DNA insertional mutagenesis
GENETICA
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dc.title.none.fl_str_mv Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny
title Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny
spellingShingle Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny
Pérez Martín, F.
Tomato
Pollen development
Tapetum degradation
Mediator complex
T-DNA insertional mutagenesis
GENETICA
title_short Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny
title_full Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny
title_fullStr Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny
title_full_unstemmed Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny
title_sort Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny
dc.creator.none.fl_str_mv Pérez Martín, F.
Juan Yuste-Lisbona, Fernando
Pineda, B.
García Sogo, Begoña
Del Olmo, I.
Alché, Juan de Dios
Egea, Isabel
Flores, F.B.
Piñeiro, M.
Jarillo,Jose A.
Angosto, T.
Capel, J.
Lozano, R.
Moreno Ferrero, Vicente|||0000-0002-0345-7300
author Pérez Martín, F.
author_facet Pérez Martín, F.
Juan Yuste-Lisbona, Fernando
Pineda, B.
García Sogo, Begoña
Del Olmo, I.
Alché, Juan de Dios
Egea, Isabel
Flores, F.B.
Piñeiro, M.
Jarillo,Jose A.
Angosto, T.
Capel, J.
Lozano, R.
Moreno Ferrero, Vicente|||0000-0002-0345-7300
author_role author
author2 Juan Yuste-Lisbona, Fernando
Pineda, B.
García Sogo, Begoña
Del Olmo, I.
Alché, Juan de Dios
Egea, Isabel
Flores, F.B.
Piñeiro, M.
Jarillo,Jose A.
Angosto, T.
Capel, J.
Lozano, R.
Moreno Ferrero, Vicente|||0000-0002-0345-7300
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Junta de Andalucía
Ministerio de Economía y Competitividad
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Tomato
Pollen development
Tapetum degradation
Mediator complex
T-DNA insertional mutagenesis
GENETICA
topic Tomato
Pollen development
Tapetum degradation
Mediator complex
T-DNA insertional mutagenesis
GENETICA
description [EN] Pollen development is a crucial step in higher plants, which not only makes possible plant fertilization and seed formation, but also determines fruit quality and yield in crop species. Here, we reported a tomato T-DNA mutant, pollen deficient1 (pod1), characterized by an abnormal anther development and the lack of viable pollen formation, which led to the production of parthenocarpic fruits. Genomic analyses and the characterization of silencing lines proved that pod1 mutant phenotype relies on the tomato SlMED18 gene encoding the subunit 18 of Mediator multi-protein complex involved in RNA polymerase II transcription machinery. The loss of SlMED18 function delayed tapetum degeneration, which resulted in deficient microspore development and scarce production of viable pollen. A detailed histological characterization of anther development proved that changes during microgametogenesis and a significant delay in tapetum degeneration are associated with a high proportion of degenerated cells and, hence, should be responsible for the low production of functional pollen grains. Expression of pollen marker genes indicated that SlMED18 is essential for the proper transcription of a subset of genes specifically required to pollen formation and fruit development, revealing a key role of SlMED18 in male gametogenesis of tomato. Additionally, SlMED18 is able to rescue developmental abnormalities of the Arabidopsis med18 mutant, indicating that most biological functions have been conserved in both species. Significance Statement Pollination is a key development process in the life cycle of flowering plants. Genetic and molecular characterization of a tomato mutant have led to the identification of POD1 gene encoding the Mediator complex subunit MED18 whose function is required for tapetum tissue degeneration, a crucial step for pollen development. Furthermore, we show that MED18 fulfils an essential role in tomato, ensuring proper gene regulation during pollen ontogeny.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/147423
url https://riunet.upv.es/handle/10251/147423
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2015-64991-C3-2-R GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2015-64991-C3-1-R GENOMICA FUNCIONAL Y MEJORA GENETICA DE LA PRODUCTIVIDAD DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO-ESTRES ABIOTICO
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2013-43098-R REGULACION MEDIADA POR CROMATINA DE CARACTERES DE DESARROLLO QUE AFECTAN AL RENDIMIENTO DE CULTIVOS DE BRASSICACEAE
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BFU2016-77243-P CARACTERIZACION Y ANALISIS FUNCIONAL DE LOS ENZIMAS RESPONSABLES DEL METABOLISMO DEL GLUTATION EN LA BIOLOGIA REPRODUCTIVA DEL OLIVO (OLEA EUROPAEA L.)
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2016-77559-R CARACTERES DE DESARROLLO REGULADOS POR CROMATINA CON INFLUENCIA EN EL RENDIMIENTO DE CULTIVOS: DISECCION DEL TIEMPO DE FLORACION EN BRASSICACEAE
Junta de Andalucía http://dx.doi.org/10.13039/501100011011 P12-AGR-1482
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2015-64991-C3-3-R GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
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spelling Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogenyPérez Martín, F.Juan Yuste-Lisbona, FernandoPineda, B.García Sogo, BegoñaDel Olmo, I.Alché, Juan de DiosEgea, IsabelFlores, F.B.Piñeiro, M.Jarillo,Jose A.Angosto, T.Capel, J.Lozano, R.Moreno Ferrero, Vicente|||0000-0002-0345-7300TomatoPollen developmentTapetum degradationMediator complexT-DNA insertional mutagenesisGENETICA[EN] Pollen development is a crucial step in higher plants, which not only makes possible plant fertilization and seed formation, but also determines fruit quality and yield in crop species. Here, we reported a tomato T-DNA mutant, pollen deficient1 (pod1), characterized by an abnormal anther development and the lack of viable pollen formation, which led to the production of parthenocarpic fruits. Genomic analyses and the characterization of silencing lines proved that pod1 mutant phenotype relies on the tomato SlMED18 gene encoding the subunit 18 of Mediator multi-protein complex involved in RNA polymerase II transcription machinery. The loss of SlMED18 function delayed tapetum degeneration, which resulted in deficient microspore development and scarce production of viable pollen. A detailed histological characterization of anther development proved that changes during microgametogenesis and a significant delay in tapetum degeneration are associated with a high proportion of degenerated cells and, hence, should be responsible for the low production of functional pollen grains. Expression of pollen marker genes indicated that SlMED18 is essential for the proper transcription of a subset of genes specifically required to pollen formation and fruit development, revealing a key role of SlMED18 in male gametogenesis of tomato. Additionally, SlMED18 is able to rescue developmental abnormalities of the Arabidopsis med18 mutant, indicating that most biological functions have been conserved in both species. Significance Statement Pollination is a key development process in the life cycle of flowering plants. Genetic and molecular characterization of a tomato mutant have led to the identification of POD1 gene encoding the Mediator complex subunit MED18 whose function is required for tapetum tissue degeneration, a crucial step for pollen development. Furthermore, we show that MED18 fulfils an essential role in tomato, ensuring proper gene regulation during pollen ontogeny.This research was supported by the Spanish Ministry of Economy and Competitiveness (grants AGL2015-64991-C3-1-R, AGL2015-64991-C3-2-R, AGL2015-64991-C3-3-R, BIO2013-43098-R, BFU2016-77243-P and BIO2016-77559-R) and Junta de Andalucia (grant P12-AGR-1482).Blackwell PublishingInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalJunta de AndalucíaMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20182018-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/147423reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2015-64991-C3-2-R GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICOMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2015-64991-C3-1-R GENOMICA FUNCIONAL Y MEJORA GENETICA DE LA PRODUCTIVIDAD DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO-ESTRES ABIOTICOMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2013-43098-R REGULACION MEDIADA POR CROMATINA DE CARACTERES DE DESARROLLO QUE AFECTAN AL RENDIMIENTO DE CULTIVOS DE BRASSICACEAEMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BFU2016-77243-P CARACTERIZACION Y ANALISIS FUNCIONAL DE LOS ENZIMAS RESPONSABLES DEL METABOLISMO DEL GLUTATION EN LA BIOLOGIA REPRODUCTIVA DEL OLIVO (OLEA EUROPAEA L.)Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2016-77559-R CARACTERES DE DESARROLLO REGULADOS POR CROMATINA CON INFLUENCIA EN EL RENDIMIENTO DE CULTIVOS: DISECCION DEL TIEMPO DE FLORACION EN BRASSICACEAEJunta de Andalucía http://dx.doi.org/10.13039/501100011011 P12-AGR-1482Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2015-64991-C3-3-R GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICOopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1474232026-06-13T07:49:27Z
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