miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation

This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers...

Descripción completa

Detalles Bibliográficos
Autores: Viñas Muñoz, José Luis, Ventayol Espinazo, Marina, Brüne, Bernhard, 1957-, Jung, Michaela, Sola Martínez, Anna, Pi i Siqués, Felip, Mastora, Chrysoula, Hotter Corripio, Georgina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/119377
Acceso en línea:https://hdl.handle.net/2445/119377
Access Level:acceso abierto
Palabra clave:Cèl·lules mare embrionàries
Micro RNAs
Diferenciació cel·lular
Embryonic stem cells
MicroRNAs
Cell diferentiation
id ES_e83f1074f8a01a2af8a36bfa762ebe51
oai_identifier_str oai:diposit.ub.edu:2445/119377
network_acronym_str ES
network_name_str España
repository_id_str
spelling miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiationViñas Muñoz, José LuisVentayol Espinazo, MarinaBrüne, Bernhard, 1957-Jung, MichaelaSola Martínez, AnnaPi i Siqués, FelipMastora, ChrysoulaHotter Corripio, GeorginaCèl·lules mare embrionàriesMicro RNAsDiferenciació cel·lularEmbryonic stem cellsMicroRNAsCell diferentiationThis study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.Public Library of Science (PLoS)2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/119377Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0060937PLoS One, 2013, vol. 8, num. 4, p. e60937https://doi.org/10.1371/journal.pone.0060937cc-by (c) Viñas Muñoz, José Luis et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1193772026-05-27T06:46:51Z
dc.title.none.fl_str_mv miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
title miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
spellingShingle miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
Viñas Muñoz, José Luis
Cèl·lules mare embrionàries
Micro RNAs
Diferenciació cel·lular
Embryonic stem cells
MicroRNAs
Cell diferentiation
title_short miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
title_full miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
title_fullStr miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
title_full_unstemmed miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
title_sort miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
dc.creator.none.fl_str_mv Viñas Muñoz, José Luis
Ventayol Espinazo, Marina
Brüne, Bernhard, 1957-
Jung, Michaela
Sola Martínez, Anna
Pi i Siqués, Felip
Mastora, Chrysoula
Hotter Corripio, Georgina
author Viñas Muñoz, José Luis
author_facet Viñas Muñoz, José Luis
Ventayol Espinazo, Marina
Brüne, Bernhard, 1957-
Jung, Michaela
Sola Martínez, Anna
Pi i Siqués, Felip
Mastora, Chrysoula
Hotter Corripio, Georgina
author_role author
author2 Ventayol Espinazo, Marina
Brüne, Bernhard, 1957-
Jung, Michaela
Sola Martínez, Anna
Pi i Siqués, Felip
Mastora, Chrysoula
Hotter Corripio, Georgina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules mare embrionàries
Micro RNAs
Diferenciació cel·lular
Embryonic stem cells
MicroRNAs
Cell diferentiation
topic Cèl·lules mare embrionàries
Micro RNAs
Diferenciació cel·lular
Embryonic stem cells
MicroRNAs
Cell diferentiation
description This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/119377
url https://hdl.handle.net/2445/119377
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0060937
PLoS One, 2013, vol. 8, num. 4, p. e60937
https://doi.org/10.1371/journal.pone.0060937
dc.rights.none.fl_str_mv cc-by (c) Viñas Muñoz, José Luis et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Viñas Muñoz, José Luis et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422925072826368
score 15,300719

Similares en LA Referencia