A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.

Blanco-Domínguez, Rafael; Sánchez-Díaz, Raquel; de la Fuente, Hortensia; Jiménez-Borreguero, Luis J; Matesanz-Marín, Adela; Relaño, Marta; Jiménez-Alejandre, Rosa; Linillos-Pradillo, Beatriz; Tsilingiri, Katerina; Martín-Mariscal, María L; Alonso-Herranz, Laura; Moreno, Guillermo; Martín-Asenjo, Roberto; García-Guimaraes, Marcos M; Bruno, Katelyn A; Dauden, Esteban; González-Álvaro, Isidoro; Villar-Guimerans, Luisa M; Martínez-León, Amaia; Salvador-Garicano, Ane M; Michelhaugh, Sam A; Ibrahim, Nasrien E; Januzzi, James L; Kottwitz, Jan; Iliceto, Sabino; Plebani, Mario; Basso, Cristina; Baritussio, Anna; Seguso, Mara; Marcolongo, Renzo; Ricote, Mercedes; Fairweather, DeLisa; Bueno, Hector; Fernández-Friera, Leticia; Alfonso, Fernando; Caforio, Alida L P; Pascual-Figal, Domingo A; Heidecker, Bettina; Lüscher, Thomas F; Das, Saumya; Fuster, Valentin; Ibáñez, Borja; Sánchez-Madrid, Francisco; Martin, Pilar

A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.

The diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging (which is not universally available). Additional approaches to diagnosis are desirable. We sought to identify a novel microRNA for the diagnosis of acut...

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Autores:	Blanco-Domínguez, Rafael, Sánchez-Díaz, Raquel, de la Fuente, Hortensia, Jiménez-Borreguero, Luis J, Matesanz-Marín, Adela, Relaño, Marta, Jiménez-Alejandre, Rosa, Linillos-Pradillo, Beatriz, Tsilingiri, Katerina, Martín-Mariscal, María L, Alonso-Herranz, Laura, Moreno, Guillermo, Martín-Asenjo, Roberto, García-Guimaraes, Marcos M, Bruno, Katelyn A, Dauden, Esteban, González-Álvaro, Isidoro, Villar-Guimerans, Luisa M, Martínez-León, Amaia, Salvador-Garicano, Ane M, Michelhaugh, Sam A, Ibrahim, Nasrien E, Januzzi, James L, Kottwitz, Jan, Iliceto, Sabino, Plebani, Mario, Basso, Cristina, Baritussio, Anna, Seguso, Mara, Marcolongo, Renzo, Ricote, Mercedes, Fairweather, DeLisa, Bueno, Hector, Fernández-Friera, Leticia, Alfonso, Fernando, Caforio, Alida L P, Pascual-Figal, Domingo A, Heidecker, Bettina, Lüscher, Thomas F, Das, Saumya, Fuster, Valentin, Ibáñez, Borja, Sánchez-Madrid, Francisco, Martin, Pilar
Tipo de recurso:	artículo
Fecha de publicación:	2021
País:	España
Institución:	Instituto de Salud Carlos III (ISCIII)
Repositorio:	Repisalud
Idioma:	inglés
OAI Identifier:	oai:repisalud.isciii.es:20.500.12105/13980
Acceso en línea:	http://hdl.handle.net/20.500.12105/13980
Access Level:	acceso abierto
Palabra clave:	Animals Autoimmune Diseases Biomarkers CD4 Antigens Circulating MicroRNA Diagnosis, Differential Disease Models, Animal Humans Mice Mice, Inbred BALB C Mice, Knockout MicroRNAs Myocardial Infarction Myocarditis Polymerase Chain Reaction ROC Curve T-Lymphocytes Th17 Cells

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oai_identifier_str	oai:repisalud.isciii.es:20.500.12105/13980
network_acronym_str	ES
network_name_str	España
repository_id_str
dc.title.none.fl_str_mv	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.
title	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.
spellingShingle	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis. Blanco-Domínguez, Rafael Animals Autoimmune Diseases Biomarkers CD4 Antigens Circulating MicroRNA Diagnosis, Differential Disease Models, Animal Humans Mice Mice, Inbred BALB C Mice, Knockout MicroRNAs Myocardial Infarction Myocarditis Polymerase Chain Reaction ROC Curve T-Lymphocytes Th17 Cells
title_short	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.
title_full	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.
title_fullStr	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.
title_full_unstemmed	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.
title_sort	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.
dc.creator.none.fl_str_mv	Blanco-Domínguez, Rafael Sánchez-Díaz, Raquel de la Fuente, Hortensia Jiménez-Borreguero, Luis J Matesanz-Marín, Adela Relaño, Marta Jiménez-Alejandre, Rosa Linillos-Pradillo, Beatriz Tsilingiri, Katerina Martín-Mariscal, María L Alonso-Herranz, Laura Moreno, Guillermo Martín-Asenjo, Roberto García-Guimaraes, Marcos M Bruno, Katelyn A Dauden, Esteban González-Álvaro, Isidoro Villar-Guimerans, Luisa M Martínez-León, Amaia Salvador-Garicano, Ane M Michelhaugh, Sam A Ibrahim, Nasrien E Januzzi, James L Kottwitz, Jan Iliceto, Sabino Plebani, Mario Basso, Cristina Baritussio, Anna Seguso, Mara Marcolongo, Renzo Ricote, Mercedes Fairweather, DeLisa Bueno, Hector Fernández-Friera, Leticia Alfonso, Fernando Caforio, Alida L P Pascual-Figal, Domingo A Heidecker, Bettina Lüscher, Thomas F Das, Saumya Fuster, Valentin Ibáñez, Borja Sánchez-Madrid, Francisco Martin, Pilar
author	Blanco-Domínguez, Rafael
author_facet	Blanco-Domínguez, Rafael Sánchez-Díaz, Raquel de la Fuente, Hortensia Jiménez-Borreguero, Luis J Matesanz-Marín, Adela Relaño, Marta Jiménez-Alejandre, Rosa Linillos-Pradillo, Beatriz Tsilingiri, Katerina Martín-Mariscal, María L Alonso-Herranz, Laura Moreno, Guillermo Martín-Asenjo, Roberto García-Guimaraes, Marcos M Bruno, Katelyn A Dauden, Esteban González-Álvaro, Isidoro Villar-Guimerans, Luisa M Martínez-León, Amaia Salvador-Garicano, Ane M Michelhaugh, Sam A Ibrahim, Nasrien E Januzzi, James L Kottwitz, Jan Iliceto, Sabino Plebani, Mario Basso, Cristina Baritussio, Anna Seguso, Mara Marcolongo, Renzo Ricote, Mercedes Fairweather, DeLisa Bueno, Hector Fernández-Friera, Leticia Alfonso, Fernando Caforio, Alida L P Pascual-Figal, Domingo A Heidecker, Bettina Lüscher, Thomas F Das, Saumya Fuster, Valentin Ibáñez, Borja Sánchez-Madrid, Francisco Martin, Pilar
author_role	author
author2	Sánchez-Díaz, Raquel de la Fuente, Hortensia Jiménez-Borreguero, Luis J Matesanz-Marín, Adela Relaño, Marta Jiménez-Alejandre, Rosa Linillos-Pradillo, Beatriz Tsilingiri, Katerina Martín-Mariscal, María L Alonso-Herranz, Laura Moreno, Guillermo Martín-Asenjo, Roberto García-Guimaraes, Marcos M Bruno, Katelyn A Dauden, Esteban González-Álvaro, Isidoro Villar-Guimerans, Luisa M Martínez-León, Amaia Salvador-Garicano, Ane M Michelhaugh, Sam A Ibrahim, Nasrien E Januzzi, James L Kottwitz, Jan Iliceto, Sabino Plebani, Mario Basso, Cristina Baritussio, Anna Seguso, Mara Marcolongo, Renzo Ricote, Mercedes Fairweather, DeLisa Bueno, Hector Fernández-Friera, Leticia Alfonso, Fernando Caforio, Alida L P Pascual-Figal, Domingo A Heidecker, Bettina Lüscher, Thomas F Das, Saumya Fuster, Valentin Ibáñez, Borja Sánchez-Madrid, Francisco Martin, Pilar
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.contributor.none.fl_str_mv	Ministerio de Ciencia e Innovación (España) Instituto de Salud Carlos III Comunidad de Madrid (España) Fundación La Marató TV3 Unión Europea. Comisión Europea. European Research Council (ERC) Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) Fundación BBVA
dc.subject.none.fl_str_mv	Animals Autoimmune Diseases Biomarkers CD4 Antigens Circulating MicroRNA Diagnosis, Differential Disease Models, Animal Humans Mice Mice, Inbred BALB C Mice, Knockout MicroRNAs Myocardial Infarction Myocarditis Polymerase Chain Reaction ROC Curve T-Lymphocytes Th17 Cells
topic	Animals Autoimmune Diseases Biomarkers CD4 Antigens Circulating MicroRNA Diagnosis, Differential Disease Models, Animal Humans Mice Mice, Inbred BALB C Mice, Knockout MicroRNAs Myocardial Infarction Myocarditis Polymerase Chain Reaction ROC Curve T-Lymphocytes Th17 Cells
description	The diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging (which is not universally available). Additional approaches to diagnosis are desirable. We sought to identify a novel microRNA for the diagnosis of acute myocarditis. To identify a microRNA specific for myocarditis, we performed microRNA microarray analyses and quantitative polymerase-chain-reaction (qPCR) assays in sorted CD4+ T cells and type 17 helper T (Th17) cells after inducing experimental autoimmune myocarditis or myocardial infarction in mice. We also performed qPCR in samples from coxsackievirus-induced myocarditis in mice. We then identified the human homologue for this microRNA and compared its expression in plasma obtained from patients with acute myocarditis with the expression in various controls. We confirmed that Th17 cells, which are characterized by the production of interleukin-17, are a characteristic feature of myocardial injury in the acute phase of myocarditis. The microRNA mmu-miR-721 was synthesized by Th17 cells and was present in the plasma of mice with acute autoimmune or viral myocarditis but not in those with acute myocardial infarction. The human homologue, designated hsa-miR-Chr8:96, was identified in four independent cohorts of patients with myocarditis. The area under the receiver-operating-characteristic curve for this novel microRNA for distinguishing patients with acute myocarditis from those with myocardial infarction was 0.927 (95% confidence interval, 0.879 to 0.975). The microRNA retained its diagnostic value in models after adjustment for age, sex, ejection fraction, and serum troponin level. After identifying a novel microRNA in mice and humans with myocarditis, we found that the human homologue (hsa-miR-Chr8:96) could be used to distinguish patients with myocarditis from those with myocardial infarction. (Funded by the Spanish Ministry of Science and Innovation and others.).
publishDate	2021
dc.date.none.fl_str_mv	2021 2021-05-27 2021 2021-05-27 2022 2022-04-13
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12105/13980
url	http://hdl.handle.net/20.500.12105/13980
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	European Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme ERC-2011-AdG 294340-GENTRIS
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Massachusetts Medical Society
publisher.none.fl_str_mv	Massachusetts Medical Society
dc.source.none.fl_str_mv	reponame:Repisalud instname:Instituto de Salud Carlos III (ISCIII)
instname_str	Instituto de Salud Carlos III (ISCIII)
reponame_str	Repisalud
collection	Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_	1869422923987550208
spelling	A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.Blanco-Domínguez, RafaelSánchez-Díaz, Raquelde la Fuente, HortensiaJiménez-Borreguero, Luis JMatesanz-Marín, AdelaRelaño, MartaJiménez-Alejandre, RosaLinillos-Pradillo, BeatrizTsilingiri, KaterinaMartín-Mariscal, María LAlonso-Herranz, LauraMoreno, GuillermoMartín-Asenjo, RobertoGarcía-Guimaraes, Marcos MBruno, Katelyn ADauden, EstebanGonzález-Álvaro, IsidoroVillar-Guimerans, Luisa MMartínez-León, AmaiaSalvador-Garicano, Ane MMichelhaugh, Sam AIbrahim, Nasrien EJanuzzi, James LKottwitz, JanIliceto, SabinoPlebani, MarioBasso, CristinaBaritussio, AnnaSeguso, MaraMarcolongo, RenzoRicote, MercedesFairweather, DeLisaBueno, HectorFernández-Friera, LeticiaAlfonso, FernandoCaforio, Alida L PPascual-Figal, Domingo AHeidecker, BettinaLüscher, Thomas FDas, SaumyaFuster, ValentinIbáñez, BorjaSánchez-Madrid, FranciscoMartin, PilarAnimalsAutoimmune DiseasesBiomarkersCD4 AntigensCirculating MicroRNADiagnosis, DifferentialDisease Models, AnimalHumansMiceMice, Inbred BALB CMice, KnockoutMicroRNAsMyocardial InfarctionMyocarditisPolymerase Chain ReactionROC CurveT-LymphocytesTh17 CellsThe diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging (which is not universally available). Additional approaches to diagnosis are desirable. We sought to identify a novel microRNA for the diagnosis of acute myocarditis. To identify a microRNA specific for myocarditis, we performed microRNA microarray analyses and quantitative polymerase-chain-reaction (qPCR) assays in sorted CD4+ T cells and type 17 helper T (Th17) cells after inducing experimental autoimmune myocarditis or myocardial infarction in mice. We also performed qPCR in samples from coxsackievirus-induced myocarditis in mice. We then identified the human homologue for this microRNA and compared its expression in plasma obtained from patients with acute myocarditis with the expression in various controls. We confirmed that Th17 cells, which are characterized by the production of interleukin-17, are a characteristic feature of myocardial injury in the acute phase of myocarditis. The microRNA mmu-miR-721 was synthesized by Th17 cells and was present in the plasma of mice with acute autoimmune or viral myocarditis but not in those with acute myocardial infarction. The human homologue, designated hsa-miR-Chr8:96, was identified in four independent cohorts of patients with myocarditis. The area under the receiver-operating-characteristic curve for this novel microRNA for distinguishing patients with acute myocarditis from those with myocardial infarction was 0.927 (95% confidence interval, 0.879 to 0.975). The microRNA retained its diagnostic value in models after adjustment for age, sex, ejection fraction, and serum troponin level. After identifying a novel microRNA in mice and humans with myocarditis, we found that the human homologue (hsa-miR-Chr8:96) could be used to distinguish patients with myocarditis from those with myocardial infarction. (Funded by the Spanish Ministry of Science and Innovation and others.).Massachusetts Medical SocietyMinisterio de Ciencia e Innovación (España)Instituto de Salud Carlos IIIComunidad de Madrid (España)Fundación La Marató TV3Unión Europea. Comisión Europea. European Research Council (ERC)Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)Fundación BBVA20222022-04-1320212021-05-2720212021-05-27journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/13980reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengEuropean Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme ERC-2011-AdG 294340-GENTRISopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/139802026-06-12T12:43:37Z
score	15,81155

A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.

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