Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties

The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitoch...

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Detalhes bibliográficos
Autores: Schiaffarino, Olivia, Valdivieso González, David, García Pérez, Inés M., Peñalva, Daniel A., Almendro Vedia, Víctor Galileo, Natale, Paolo, López Montero, Iván
Tipo de documento: artigo
Data de publicação:2022
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositório:Docta Complutense
Idioma:inglês
OAI Identifier:oai:docta.ucm.es:20.500.14352/115838
Acesso em linha:https://hdl.handle.net/20.500.14352/115838
Access Level:Acceso aberto
Palavra-chave:544
Outer mitochondrial membrane
Inner mitochondrial membrane
Langmuir monolayers
Supported lipid bilayers
Giant unilamellar vesicles (GUV)
FRAP
Micropipettes
Mitochondria
Ciencias
Ciencias Biomédicas
Física (Física)
Química
Biología
24 Ciencias de la Vida
2406 Biofísica
2210 Química Física
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oai_identifier_str oai:docta.ucm.es:20.500.14352/115838
network_acronym_str ES
network_name_str España
repository_id_str
spelling Mitochondrial membrane models built from native lipid extracts: Interfacial and transport propertiesSchiaffarino, OliviaValdivieso González, DavidGarcía Pérez, Inés M.Peñalva, Daniel A.Almendro Vedia, Víctor GalileoNatale, PaoloLópez Montero, Iván544Outer mitochondrial membraneInner mitochondrial membraneLangmuir monolayersSupported lipid bilayersGiant unilamellar vesicles (GUV)FRAPMicropipettesMitochondriaCienciasCiencias BiomédicasFísica (Física)QuímicaBiología24 Ciencias de la Vida2406 Biofísica2210 Química FísicaThe mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.FRONTIERS MEDIA SAUniversidad Complutense de Madrid20222022-09-2320222022-09-23journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/115838reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1158382026-06-02T12:44:21Z
dc.title.none.fl_str_mv Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
spellingShingle Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
Schiaffarino, Olivia
544
Outer mitochondrial membrane
Inner mitochondrial membrane
Langmuir monolayers
Supported lipid bilayers
Giant unilamellar vesicles (GUV)
FRAP
Micropipettes
Mitochondria
Ciencias
Ciencias Biomédicas
Física (Física)
Química
Biología
24 Ciencias de la Vida
2406 Biofísica
2210 Química Física
title_short Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title_full Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title_fullStr Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title_full_unstemmed Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title_sort Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
dc.creator.none.fl_str_mv Schiaffarino, Olivia
Valdivieso González, David
García Pérez, Inés M.
Peñalva, Daniel A.
Almendro Vedia, Víctor Galileo
Natale, Paolo
López Montero, Iván
author Schiaffarino, Olivia
author_facet Schiaffarino, Olivia
Valdivieso González, David
García Pérez, Inés M.
Peñalva, Daniel A.
Almendro Vedia, Víctor Galileo
Natale, Paolo
López Montero, Iván
author_role author
author2 Valdivieso González, David
García Pérez, Inés M.
Peñalva, Daniel A.
Almendro Vedia, Víctor Galileo
Natale, Paolo
López Montero, Iván
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 544
Outer mitochondrial membrane
Inner mitochondrial membrane
Langmuir monolayers
Supported lipid bilayers
Giant unilamellar vesicles (GUV)
FRAP
Micropipettes
Mitochondria
Ciencias
Ciencias Biomédicas
Física (Física)
Química
Biología
24 Ciencias de la Vida
2406 Biofísica
2210 Química Física
topic 544
Outer mitochondrial membrane
Inner mitochondrial membrane
Langmuir monolayers
Supported lipid bilayers
Giant unilamellar vesicles (GUV)
FRAP
Micropipettes
Mitochondria
Ciencias
Ciencias Biomédicas
Física (Física)
Química
Biología
24 Ciencias de la Vida
2406 Biofísica
2210 Química Física
description The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-09-23
2022
2022-09-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/115838
url https://hdl.handle.net/20.500.14352/115838
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv FRONTIERS MEDIA SA
publisher.none.fl_str_mv FRONTIERS MEDIA SA
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422917326995456
score 15,811543

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