Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis

Despite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profle and afect their results. This study determines if the blood starting mater...

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Autores: Mompeon, Ana, Ortega Paz, Luis, Vidal Gomez, Xavier, Januario Costa, Tiago, Perez Cremades, Daniel, Garcia Blas, Sergio, Brugaletta, Salvatore, Sanchis Forés, Juan, Sabaté Tenas, Manuel, Novella, Susana, Dantas, Ana Paula, Hermenegildo, Carlos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/186907
Acceso en línea:https://hdl.handle.net/2445/186907
Access Level:acceso abierto
Palabra clave:Vellesa
Micro RNAs
Infart de miocardi
Plasma sanguini
Old age
MicroRNAs
Myocardial infarction
Blood plasma
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spelling Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysisMompeon, AnaOrtega Paz, LuisVidal Gomez, XavierJanuario Costa, TiagoPerez Cremades, DanielGarcia Blas, SergioBrugaletta, SalvatoreSanchis Forés, JuanSabaté Tenas, ManuelNovella, SusanaDantas, Ana PaulaHermenegildo, CarlosVellesaMicro RNAsInfart de miocardiPlasma sanguiniOld ageMicroRNAsMyocardial infarctionBlood plasmaDespite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profle and afect their results. This study determines if the blood starting material is a source of variance in miRNA profle by performing a paired comparison in plasma and serum of the expression of primary miRNAs associated with CVD. Circulating miRNA yield was similar in both plasma and serum, although a signifcant increase was observed in patients with Non-ST-elevation myocardial infarction (NSTEMI) compared to control volunteers. When normalized by the expression of miR-484, diferent patterns of miRNA expression between serum and plasma. Although NSTEMI modifed the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p<0.01). For miR-133a and miR-26a, diferences were only detected in serum (p=0.0240), and conversely, miR-499a showed diferences only in plasma of NSTEMI (p=0.001). Interestingly, miR-21 showed an opposite pattern of expression, being increased in serum (2−ΔΔCt : 5.7, p=0.0221) and decreased in plasma (2−ΔΔCt : 0.5, p=0.0107). Plasma and serum exhibit diferent patterns of circulating miRNA expression in NSTEMI and suggest that results from studies with diferent starting material could not be comparable.Nature Publishing Group2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/186907Articles publicats en revistes (Medicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1038/s41598-020-61507-zScientific Reports, 2020, vol. 10, num. 1, p. 5373https://doi.org/10.1038/s41598-020-61507-zcc-by (c) Mompeon, Ana et al., 2020https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1869072026-05-27T06:46:51Z
dc.title.none.fl_str_mv Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
title Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
spellingShingle Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
Mompeon, Ana
Vellesa
Micro RNAs
Infart de miocardi
Plasma sanguini
Old age
MicroRNAs
Myocardial infarction
Blood plasma
title_short Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
title_full Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
title_fullStr Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
title_full_unstemmed Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
title_sort Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
dc.creator.none.fl_str_mv Mompeon, Ana
Ortega Paz, Luis
Vidal Gomez, Xavier
Januario Costa, Tiago
Perez Cremades, Daniel
Garcia Blas, Sergio
Brugaletta, Salvatore
Sanchis Forés, Juan
Sabaté Tenas, Manuel
Novella, Susana
Dantas, Ana Paula
Hermenegildo, Carlos
author Mompeon, Ana
author_facet Mompeon, Ana
Ortega Paz, Luis
Vidal Gomez, Xavier
Januario Costa, Tiago
Perez Cremades, Daniel
Garcia Blas, Sergio
Brugaletta, Salvatore
Sanchis Forés, Juan
Sabaté Tenas, Manuel
Novella, Susana
Dantas, Ana Paula
Hermenegildo, Carlos
author_role author
author2 Ortega Paz, Luis
Vidal Gomez, Xavier
Januario Costa, Tiago
Perez Cremades, Daniel
Garcia Blas, Sergio
Brugaletta, Salvatore
Sanchis Forés, Juan
Sabaté Tenas, Manuel
Novella, Susana
Dantas, Ana Paula
Hermenegildo, Carlos
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Vellesa
Micro RNAs
Infart de miocardi
Plasma sanguini
Old age
MicroRNAs
Myocardial infarction
Blood plasma
topic Vellesa
Micro RNAs
Infart de miocardi
Plasma sanguini
Old age
MicroRNAs
Myocardial infarction
Blood plasma
description Despite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profle and afect their results. This study determines if the blood starting material is a source of variance in miRNA profle by performing a paired comparison in plasma and serum of the expression of primary miRNAs associated with CVD. Circulating miRNA yield was similar in both plasma and serum, although a signifcant increase was observed in patients with Non-ST-elevation myocardial infarction (NSTEMI) compared to control volunteers. When normalized by the expression of miR-484, diferent patterns of miRNA expression between serum and plasma. Although NSTEMI modifed the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p<0.01). For miR-133a and miR-26a, diferences were only detected in serum (p=0.0240), and conversely, miR-499a showed diferences only in plasma of NSTEMI (p=0.001). Interestingly, miR-21 showed an opposite pattern of expression, being increased in serum (2−ΔΔCt : 5.7, p=0.0221) and decreased in plasma (2−ΔΔCt : 0.5, p=0.0107). Plasma and serum exhibit diferent patterns of circulating miRNA expression in NSTEMI and suggest that results from studies with diferent starting material could not be comparable.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/186907
url https://hdl.handle.net/2445/186907
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-61507-z
Scientific Reports, 2020, vol. 10, num. 1, p. 5373
https://doi.org/10.1038/s41598-020-61507-z
dc.rights.none.fl_str_mv cc-by (c) Mompeon, Ana et al., 2020
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Mompeon, Ana et al., 2020
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422909655613440
score 15.301603

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