Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility
Biopolymers play a critical role as scaffolds used in tendon and ligament (TL) regeneration. Although advanced biopolymer materials have been proposed with optimised mechanical properties, biocompatibility, degradation, and processability, it is still challenging to find the right balance between th...
| Autores: | , , , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Recursos: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/68091 |
| Acesso em linha: | https://hdl.handle.net/10171/68091 |
| Access Level: | acceso abierto |
| Palavra-chave: | Anterior cruciate ligament Biocomposite Biodegradable scaffolds Fibre extrusion Silk Tendon graft |
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Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibilityHeidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbbMuiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620Harrington, E. (Emma)|||/items/26d639eb-aa84-4c74-a625-d04884781db0Ruan, R. (Rui)|||/items/97ec67de-1b93-410f-a313-6ed9efbf11eeChen, P. (Peilin)|||/items/0f9a4c44-8645-45f8-9208-0a1d9c33c58eDavachi, S.M. (Seyed Mohammad)|||/items/97b47088-68bd-4e95-bbbb-a0de40b4cf41Allardyce, B. (Benjamin)|||/items/105c99ba-0e29-4190-b53e-1c23759dd9dcRajkhowa, R. (Rangam)|||/items/a5056c03-4840-4be3-8ca8-2e24ea793890Dilley, R. (Rodney)|||/items/75f2c88e-6fcb-4204-b91d-8e886b7334e8Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1aDoyle, B. (Barry)|||/items/cd05a898-8549-4ff5-a96d-f4a418fd6ea3Zheng, M. (Minghao)|||/items/92ef8bb5-77fe-43ec-8cd8-600ae7928dc5Juan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342Anterior cruciate ligamentBiocompositeBiodegradable scaffoldsFibre extrusionSilkTendon graftBiopolymers play a critical role as scaffolds used in tendon and ligament (TL) regeneration. Although advanced biopolymer materials have been proposed with optimised mechanical properties, biocompatibility, degradation, and processability, it is still challenging to find the right balance between these properties. Here, we aim to develop novel hybrid biocomposites based on poly(p-dioxanone) (PDO), poly(lactide-co-caprolactone) (LCL) and silk to produce high-performance grafts suitable for TL tissue repair. Biocomposites containing 1-15% of silk were studied through a range of characterisation techniques. We then explored biocompatibility through in vitro and in vivo studies using a mouse model. We found that adding up to 5% silk increases the tensile properties, degradation rate and miscibility between PDO and LCL phases without agglomeration of silk inside the com-posites. Furthermore, addition of silk increases surface roughness and hydrophilicity. In vitro experiments show that the silk improved attachment of tendon-derived stem cells and proliferation over 72 h, while in vivo studies indicate that the silk can reduce the expression of pro-inflammatory cytokines after six weeks of implantation. Finally, we selected a promising biocomposite and created a prototype TL graft based on extruded fibres. We found that the tensile properties of both individual fibres and braided grafts could be suitable for anterior cruciate ligament (ACL) repair applications.Dadun. Depósito Académico Digital Universidad de Navarra20232023-12-1920232023-01-0120232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/68091reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/680912026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility |
| title |
Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility |
| spellingShingle |
Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility Heidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbb Anterior cruciate ligament Biocomposite Biodegradable scaffolds Fibre extrusion Silk Tendon graft |
| title_short |
Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility |
| title_full |
Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility |
| title_fullStr |
Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility |
| title_full_unstemmed |
Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility |
| title_sort |
Novel hybrid biocomposites for tendon grafts: the addition of silk to polydioxanone and poly(lactide-co-caprolactone) enhances material properties, in vitro and in vivo biocompatibility |
| dc.creator.none.fl_str_mv |
Heidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbb Muiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620 Harrington, E. (Emma)|||/items/26d639eb-aa84-4c74-a625-d04884781db0 Ruan, R. (Rui)|||/items/97ec67de-1b93-410f-a313-6ed9efbf11ee Chen, P. (Peilin)|||/items/0f9a4c44-8645-45f8-9208-0a1d9c33c58e Davachi, S.M. (Seyed Mohammad)|||/items/97b47088-68bd-4e95-bbbb-a0de40b4cf41 Allardyce, B. (Benjamin)|||/items/105c99ba-0e29-4190-b53e-1c23759dd9dc Rajkhowa, R. (Rangam)|||/items/a5056c03-4840-4be3-8ca8-2e24ea793890 Dilley, R. (Rodney)|||/items/75f2c88e-6fcb-4204-b91d-8e886b7334e8 Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1a Doyle, B. (Barry)|||/items/cd05a898-8549-4ff5-a96d-f4a418fd6ea3 Zheng, M. (Minghao)|||/items/92ef8bb5-77fe-43ec-8cd8-600ae7928dc5 Juan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342 |
| author |
Heidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbb |
| author_facet |
Heidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbb Muiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620 Harrington, E. (Emma)|||/items/26d639eb-aa84-4c74-a625-d04884781db0 Ruan, R. (Rui)|||/items/97ec67de-1b93-410f-a313-6ed9efbf11ee Chen, P. (Peilin)|||/items/0f9a4c44-8645-45f8-9208-0a1d9c33c58e Davachi, S.M. (Seyed Mohammad)|||/items/97b47088-68bd-4e95-bbbb-a0de40b4cf41 Allardyce, B. (Benjamin)|||/items/105c99ba-0e29-4190-b53e-1c23759dd9dc Rajkhowa, R. (Rangam)|||/items/a5056c03-4840-4be3-8ca8-2e24ea793890 Dilley, R. (Rodney)|||/items/75f2c88e-6fcb-4204-b91d-8e886b7334e8 Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1a Doyle, B. (Barry)|||/items/cd05a898-8549-4ff5-a96d-f4a418fd6ea3 Zheng, M. (Minghao)|||/items/92ef8bb5-77fe-43ec-8cd8-600ae7928dc5 Juan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342 |
| author_role |
author |
| author2 |
Muiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620 Harrington, E. (Emma)|||/items/26d639eb-aa84-4c74-a625-d04884781db0 Ruan, R. (Rui)|||/items/97ec67de-1b93-410f-a313-6ed9efbf11ee Chen, P. (Peilin)|||/items/0f9a4c44-8645-45f8-9208-0a1d9c33c58e Davachi, S.M. (Seyed Mohammad)|||/items/97b47088-68bd-4e95-bbbb-a0de40b4cf41 Allardyce, B. (Benjamin)|||/items/105c99ba-0e29-4190-b53e-1c23759dd9dc Rajkhowa, R. (Rangam)|||/items/a5056c03-4840-4be3-8ca8-2e24ea793890 Dilley, R. (Rodney)|||/items/75f2c88e-6fcb-4204-b91d-8e886b7334e8 Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1a Doyle, B. (Barry)|||/items/cd05a898-8549-4ff5-a96d-f4a418fd6ea3 Zheng, M. (Minghao)|||/items/92ef8bb5-77fe-43ec-8cd8-600ae7928dc5 Juan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342 |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Anterior cruciate ligament Biocomposite Biodegradable scaffolds Fibre extrusion Silk Tendon graft |
| topic |
Anterior cruciate ligament Biocomposite Biodegradable scaffolds Fibre extrusion Silk Tendon graft |
| description |
Biopolymers play a critical role as scaffolds used in tendon and ligament (TL) regeneration. Although advanced biopolymer materials have been proposed with optimised mechanical properties, biocompatibility, degradation, and processability, it is still challenging to find the right balance between these properties. Here, we aim to develop novel hybrid biocomposites based on poly(p-dioxanone) (PDO), poly(lactide-co-caprolactone) (LCL) and silk to produce high-performance grafts suitable for TL tissue repair. Biocomposites containing 1-15% of silk were studied through a range of characterisation techniques. We then explored biocompatibility through in vitro and in vivo studies using a mouse model. We found that adding up to 5% silk increases the tensile properties, degradation rate and miscibility between PDO and LCL phases without agglomeration of silk inside the com-posites. Furthermore, addition of silk increases surface roughness and hydrophilicity. In vitro experiments show that the silk improved attachment of tendon-derived stem cells and proliferation over 72 h, while in vivo studies indicate that the silk can reduce the expression of pro-inflammatory cytokines after six weeks of implantation. Finally, we selected a promising biocomposite and created a prototype TL graft based on extruded fibres. We found that the tensile properties of both individual fibres and braided grafts could be suitable for anterior cruciate ligament (ACL) repair applications. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023-12-19 2023 2023-01-01 2023 2023-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/68091 |
| url |
https://hdl.handle.net/10171/68091 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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15.811543 |