Insulin degradation by adipose tissue is increased in human obesity

White adipose tissue samples from obese and lean patients were used for the estimation ofinsulin protease and insulin:glutathione transhydrogenase using 1251-labeled insulin. There was no activity detected in the absence of reduced glutathione, which indicates that insulin is cleaved in human adipos...

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Autores: Rafecas Jorba, Immaculada, Fernández López, José Antonio, Salinas, Isabel, Formiguera Sala, X., Remesar Betlloch, Xavier, Foz Sala, M. (Màrius), Alemany, Marià, 1946-
Tipo de recurso: artículo
Fecha de publicación:1995
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/22382
Acceso en línea:https://hdl.handle.net/2445/22382
Access Level:acceso abierto
Palabra clave:Insulina
Obesitat
Insulin
Obesity
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spelling Insulin degradation by adipose tissue is increased in human obesityRafecas Jorba, ImmaculadaFernández López, José AntonioSalinas, IsabelFormiguera Sala, X.Remesar Betlloch, XavierFoz Sala, M. (Màrius)Alemany, Marià, 1946-InsulinaObesitatInsulinObesityWhite adipose tissue samples from obese and lean patients were used for the estimation ofinsulin protease and insulin:glutathione transhydrogenase using 1251-labeled insulin. There was no activity detected in the absence of reduced glutathione, which indicates that insulin is cleaved in human adipose "tissue through reduction of the disulfide bridge between the chains. O bese patients showed higher transhydrogenase activity (per U tissue protein wt, per U tissue wt, and in the total adipose tissue mass) than the lean group. There is a significant correlation between the activity per U tissue wt, and protein and total activity in the whole adipose tissue with respect to body mass index, with a higher activity in obese patients. The potential ofinsulin cleavage by adipose tissue in obese patients was a mean 5.6-fold higher than that in controla. The coexistence of high insulinemia and high cleavage capability implies that insulin secretion and turnover are increased in the o bese. Thus, white adipose tissue may be crucial in the control of energy availability through modulation ofinsulin cleavage.The Endocrine Society1995info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2445/22382Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1210/jc.80.2.693Journal of Clinical Endocrinology and Metabolism, 1995, vol. 80, p. 693-695http://dx.doi.org/10.1210/jc.80.2.693(c) The Endocrine Society, 1995info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/223822026-05-27T06:46:51Z
dc.title.none.fl_str_mv Insulin degradation by adipose tissue is increased in human obesity
title Insulin degradation by adipose tissue is increased in human obesity
spellingShingle Insulin degradation by adipose tissue is increased in human obesity
Rafecas Jorba, Immaculada
Insulina
Obesitat
Insulin
Obesity
title_short Insulin degradation by adipose tissue is increased in human obesity
title_full Insulin degradation by adipose tissue is increased in human obesity
title_fullStr Insulin degradation by adipose tissue is increased in human obesity
title_full_unstemmed Insulin degradation by adipose tissue is increased in human obesity
title_sort Insulin degradation by adipose tissue is increased in human obesity
dc.creator.none.fl_str_mv Rafecas Jorba, Immaculada
Fernández López, José Antonio
Salinas, Isabel
Formiguera Sala, X.
Remesar Betlloch, Xavier
Foz Sala, M. (Màrius)
Alemany, Marià, 1946-
author Rafecas Jorba, Immaculada
author_facet Rafecas Jorba, Immaculada
Fernández López, José Antonio
Salinas, Isabel
Formiguera Sala, X.
Remesar Betlloch, Xavier
Foz Sala, M. (Màrius)
Alemany, Marià, 1946-
author_role author
author2 Fernández López, José Antonio
Salinas, Isabel
Formiguera Sala, X.
Remesar Betlloch, Xavier
Foz Sala, M. (Màrius)
Alemany, Marià, 1946-
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Insulina
Obesitat
Insulin
Obesity
topic Insulina
Obesitat
Insulin
Obesity
description White adipose tissue samples from obese and lean patients were used for the estimation ofinsulin protease and insulin:glutathione transhydrogenase using 1251-labeled insulin. There was no activity detected in the absence of reduced glutathione, which indicates that insulin is cleaved in human adipose "tissue through reduction of the disulfide bridge between the chains. O bese patients showed higher transhydrogenase activity (per U tissue protein wt, per U tissue wt, and in the total adipose tissue mass) than the lean group. There is a significant correlation between the activity per U tissue wt, and protein and total activity in the whole adipose tissue with respect to body mass index, with a higher activity in obese patients. The potential ofinsulin cleavage by adipose tissue in obese patients was a mean 5.6-fold higher than that in controla. The coexistence of high insulinemia and high cleavage capability implies that insulin secretion and turnover are increased in the o bese. Thus, white adipose tissue may be crucial in the control of energy availability through modulation ofinsulin cleavage.
publishDate 1995
dc.date.none.fl_str_mv 1995
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/22382
url https://hdl.handle.net/2445/22382
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1210/jc.80.2.693
Journal of Clinical Endocrinology and Metabolism, 1995, vol. 80, p. 693-695
http://dx.doi.org/10.1210/jc.80.2.693
dc.rights.none.fl_str_mv (c) The Endocrine Society, 1995
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) The Endocrine Society, 1995
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Endocrine Society
publisher.none.fl_str_mv The Endocrine Society
dc.source.none.fl_str_mv Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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